Electrical/Asymmetric Flow Field-Flow Fractionation (EAF4) System with Light Scattering Detection

具有光散射检测功能的电/非对称流场流分离 (EAF4) 系统

• 批准号: 10428944
• 负责人: ARABELA A GRIGORESCU
• 金额: $ 32.46万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10428944
• 关键词: Address; Antineoplastic Agents; Area; Biomedical Research; Biophysics; Chicago; Collection; Core Facility; Detection; Equipment; Field Flow Fractionation; Funding; Genomics; MLLT2 gene; Measurement; Measures; Minor; Modernization; Molecular Target; Multiprotein Complexes; Nanotechnology; Particle Size; Polymers; Preparation; Production; Research; Research Project Grants; Resolution; Resource Sharing; Science; Shapes; Structure; System; Technical Expertise; United States National Institutes of Health; Universities; Vaccines; Virus; extracellular vesicles; indexing; innovation; instrument; light scattering; nanocarrier; nanoparticle; particle; prospective; protein complex; targeted treatment; zeta potential

Project Summary This proposal seeks funding for the acquisition of an Electrical/Asymmetric Flow Field-Flow Fractionation (E/AF4) system with static and dynamic light scattering detection. This instrument provides state-of-the-art capabilities for integrated separation and characterization of wide range of materials used in modern biomedical research, including proteins and multi-protein complexes, extracellular vesicles, viruses and vaccines, synthetic nanoparticles, and polymeric nanocarriers. The system has analytical and preparative capabilities for high-resolution separation of particles 1 nm-1 m in size and directly and precisely measures and particle size, mass, and zeta potential. Moreover, shape polydispersity indices can be calculated from the mass and size measurements. These capabilities are required by many research projects currently underway at Northwestern University including, but not limited to, the 14 NIH-funded projects described in detail in this application. These projects encompass studies addressing various aspects of genomic organization and function, structure-activity studies to identify and characterize new molecular targets for anti-cancer drugs, and nanotechnology projects pursuing innovative approaches for targeted therapies. A total of 10 prospective major (9 with active NIH funding) and 5 minor (3 with active NIH funding) users are requesting these capabilities. This type of equipment does not exist at Northwestern University or in any core facility in the Chicago area. The instrument will be integrated in the Keck Biophysics Facility, a well-known resource for shared equipment and technical expertise at Northwestern. The facility has a 24-year track record of keeping a diverse collection of high-end research instruments operational and accessible and facilitating the production of large amounts of first- rate science.

项目摘要 本提案寻求资金用于购买电/不对称流场 具有静态和动态光散射检测的分级(E/AF4)系统。这台仪器 提供最先进的功能，用于集成分离和表征各种 现代生物医学研究中使用的材料，包括蛋白质和多蛋白质复合体， 胞外囊泡、病毒和疫苗、合成纳米颗粒和聚合物纳米载体。这个 系统具有分析和制备能力，可高分辨率分离颗粒1 nm-1m 在大小和直接和精确的测量上 和 颗粒大小、质量和Zeta电势。此外，形状 多分散性指数可以从质量和尺寸测量中计算出来。 这些能力是西北大学目前正在进行的许多研究项目所需的 包括但不限于本申请书中详细描述的14个国家卫生研究院资助的项目。 这些项目包括解决基因组组织和功能的各个方面的研究， 结构-活性研究，以确定和表征抗癌药物的新分子靶点，以及 为靶向治疗寻求创新方法的纳米技术项目。 共有10个预期主修专业(9个有NIH积极资助)和5个辅修专业(3个有NIH积极资助) 用户正在请求这些功能。西北大学没有这种类型的设备 在芝加哥地区的大学或任何核心设施。该仪器将集成在Keck中 生物物理设施，共享设备和技术专业知识的知名资源，请访问 西北大学。该工厂拥有24年的记录，保持着不同的高端产品系列 可操作和可获得的研究仪器，并促进生产大量的第一批- 评级科学。