Measurement of streaming potential for determination of capillary surface characteristics

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The proposed project aims to use a new capillary electrophoresis instrument manufactured in the local area to**characterize the surface properties of capillaries coated with various coating materials. Capillary**electrophoresis has become one of the most important analytical separation techniques used in the analysis of**biopharmaceutical industry, biomedical research, and industrial manufacturing activities. Reproducibility of**chemical analysis is essential for all analytical methods. Maintaining a chemically clean surface for the**capillary used in CE in one of the critical conditions for obtaining reproducible migration times. The applicant**will develop strategies and methods to evaluate the relationship between the streaming potential with different**capillary surface conditions, different buffer systems, and different covalent and non-covalent surface coatings.**The results will provide important guidance for researchers and other instrument users in their method**development to generate high quality and reproducible results.