Biocontrol pseudomonads: interaction with the plants they protect and grazing predators that threaten their existence
生物防治假单胞菌:与它们保护的植物和威胁其生存的食肉动物相互作用
基本信息
- 批准号:RGPIN-2017-04970
- 负责人:
- 金额:$ 2.91万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2018
- 资助国家:加拿大
- 起止时间:2018-01-01 至 2019-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
For the past 15 years, research ongoing in my lab has focused on a biocontrol strain, Pseudomonas chlororaphis PA23, which is able to protect canola from sclerotinia stem rot caused by the fungal pathogen Sclerotinia sclerotiorum (Ss). Application of PA23 to the aerial surfaces of canola inhibits Ss directly through antibiosis and indirectly through priming of the plant defense response. In the first aim of the proposed research, we will shift our attention below ground and explore how the presence of PA23 in the rhizosphere influences plant physiology. PA23 colonization and gene expression on the plant roots will be investigated together with PA23-mediated changes in canola gene expression using RNA sequencing (RNAseq). In aim 2, the role of PA23 antifungal metabolites in the interaction with the grazing predator Acanthamoeba castellani (Ac) will be explored. Co-culturing will enable us to profile the bacterial response to predator exposure through RNAseq. Aim 3 builds upon our recent discovery that PA23 not only protects plants from fungal attack but also promotes plant growth. The impact of PA23 and protozoan grazing on canola root architecture and gene expression will be revealed. Additionally, metagenomic analysis will be employed to elucidate how the presence of Ac affects PA23 persistence and the composition of the soil community as a whole. Collectively, findings from the proposed studies will provide an unprecedented understanding of how PA23 in the rhizosphere impacts canola gene expression and susceptibility to Ss infection in the phyllosphere. We will reveal for the first time the effect of Ac grazing on PA23 gene expression and plant physiology when one or both organisms are present in the rhizosphere. This research will provide graduate and undergraduate students training in a wide breadth of areas including next generation sequencing, bioinformatics, confocal microscopy and tissue culture. Such opportunities will ensure trainees are well prepared and in high demand for both the discovery and applied science job sectors. The impact of this work will be realized by scientists investigating trans-kingdom signaling in complex prokaryotic-eukaryotic interactions, growers in the form of increased crop yields and society as a whole in our quest for safe, sustainable strategies for nourishing the ever-increasing number of humans on the planet.*****
在过去的15年里,我的实验室正在进行的研究集中在一个生物控制菌株,绿针假单胞菌PA 23,这是能够保护油菜从由真菌病原体核盘菌(Ss)引起的菌核茎腐病。施用PA 23到油菜的气生表面直接通过抑制Ss和间接通过引发植物防御反应。在拟议研究的第一个目标中,我们将把注意力转移到地下,探索根际中PA 23的存在如何影响植物生理。将使用RNA测序(RNAseq)研究PA 23在植物根部的定殖和基因表达以及PA 23介导的卡诺拉基因表达变化。在目标2中,将探索PA 23抗真菌代谢产物在与食草性捕食者卡氏阿米巴(Ac)相互作用中的作用。共培养将使我们能够通过RNAseq描述细菌对捕食者暴露的反应。目标3建立在我们最近发现的基础上,PA 23不仅保护植物免受真菌侵袭,而且还促进植物生长。PA 23和原生动物放牧对油菜根结构和基因表达的影响将被揭示。此外,宏基因组学分析将阐明如何存在的Ac影响PA 23的持久性和组成的土壤群落作为一个整体。总的来说,从拟议的研究结果将提供一个前所未有的了解如何PA 23在根际影响油菜基因表达和敏感性的叶际Ss感染。我们将首次揭示当一种或两种生物都存在于根际时,AC放牧对PA 23基因表达和植物生理的影响。这项研究将为研究生和本科生提供广泛领域的培训,包括下一代测序,生物信息学,共聚焦显微镜和组织培养。这些机会将确保受训人员做好充分准备,并在发现和应用科学工作部门的高需求。这项工作的影响将通过研究复杂的原核-真核相互作用中的跨王国信号传导的科学家,以增加作物产量的形式的种植者和整个社会来实现,以寻求安全,可持续的战略来滋养地球上不断增加的人类。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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deKievit, Teresa其他文献
deKievit, Teresa的其他文献
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{{ truncateString('deKievit, Teresa', 18)}}的其他基金
Molecular mechanisms involved in Pseudomonas biocontrol
假单胞菌生物防治的分子机制
- 批准号:
249559-2012 - 财政年份:2014
- 资助金额:
$ 2.91万 - 项目类别:
Discovery Grants Program - Individual
Molecular mechanisms involved in Pseudomonas biocontrol
假单胞菌生物防治的分子机制
- 批准号:
249559-2012 - 财政年份:2013
- 资助金额:
$ 2.91万 - 项目类别:
Discovery Grants Program - Individual
Molecular mechanisms involved in Pseudomonas biocontrol
假单胞菌生物防治的分子机制
- 批准号:
249559-2012 - 财政年份:2012
- 资助金额:
$ 2.91万 - 项目类别:
Discovery Grants Program - Individual
Molecular mechanisms involved in pseudomonas biocontrol
假单胞菌生物防治的分子机制
- 批准号:
249559-2007 - 财政年份:2011
- 资助金额:
$ 2.91万 - 项目类别:
Discovery Grants Program - Individual
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