Isolation and characterization of Clostridium perfringens lytic phages for veterinary applications in the poultry industry

产气荚膜梭菌裂解噬菌体的分离和表征，用于家禽业的兽医应用

• 批准号: 529295-2018
• 负责人: Fortier, LouisCharles
• 金额: $ 1.82万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=650981
• 关键词: Isolation; characterization; Clostridium; perfringens; lytic

Necrotic enteritis caused by the toxigenic bacterium Clostridium perfringens (Cp) is a burden on animal health, especially in the poultry industry. This pathogen is also a concern for food safety as Cp can be transmitted to human through the food chain. The ban of antimicrobial growth promoter use in European countries was almost immediately followed by health problems in broiler flocks, with the most remarkable increase in acute clinical and subclinical necrotic enteritis caused by Cp. Canadian legislation on antimicrobial growth promoter use will be enforced by the end of 2018 and the Canadian poultry industry is facing the lack of suitable and efficient alternatives to prevent health problem in broiler flocks. The total global economic loss due to outbreaks of necrotic enteritis is estimated to be over $2.5 billions annually and, to date, the industry does not have any efficient remedy for the problem. With this project in collaboration with SyntBioLabo Inc., we seek to develop cocktails of lytic phages (i.e. bacterial viruses) capable of killing Cp in phage therapy applications. Although Cp phages have been isolated before and phage therapy to prevent necrotic enteritis in chickens has been demonstrated, there is little data and few Cp phages available. Moreover, these phages must target the bacterial strains currently circulating in poultry farms.**Objectives: Our first objective will consist in isolating from chicken feces Cp strains currently in circulation in 8 poultry farms of the province of Quebec. Our second objective will consist in screening fecal samples to detect virulent phages against 80-90% of the most prevalent Cp strains identified in objective 1. To reach our goals, we will use well-established microbiological and molecular methods to characterize Cp strains, as well as the isolated phages. By the end of this project, we aim to gather 15-20 lytic phages that could be combined into a cocktail. This project will reinforce our fundamental understanding of the biology, diversity, and therapeutic potential of Cp phages. Because Cp can infect both humans and animals, success of our project could represent an important milestone for future phage therapy of Cp in humans. ****

由产毒细菌产气荚膜梭菌 (Cp) 引起的坏死性肠炎对动物健康造成负担，尤其是在家禽业。这种病原体也是食品安全的一个问题，因为 Cp 可以通过食物链传播给人类。欧洲国家禁止使用抗生素生长促进剂后，肉鸡群的健康问题几乎立即出现，其中由 Cp 引起的急性临床和亚临床坏死性肠炎的增加最为显着。加拿大关于抗菌生长促进剂使用的立法将于 2018 年底强制执行，加拿大家禽业正面临缺乏合适且有效的替代品来预防肉鸡群健康问题的问题。据估计，每年因坏死性肠炎爆发造成的全球经济损失超过 25 亿美元，而迄今为止，该行业还没有任何有效的补救措施来解决这一问题。通过与 SyntBioLabo Inc. 合作的这个项目，我们寻求开发能够在噬菌体治疗应用中杀死 Cp 的裂解噬菌体（即细菌病毒）混合物。尽管之前已分离出 Cp 噬菌体，并且已证明噬菌体疗法可预防鸡坏死性肠炎，但可用的数据很少，而且可用的 Cp 噬菌体也很少。此外，这些噬菌体必须针对目前在家禽养殖场中流通的细菌菌株。**目标：我们的首要目标是从鸡粪中分离出目前在魁北克省 8 个家禽养殖场流通的 Cp 菌株。我们的第二个目标是筛选粪便样本，以检测针对目标 1 中确定的 80-90% 最流行 Cp 菌株的剧毒噬菌体。为了实现我们的目标，我们将使用成熟的微生物学和分子方法来表征 Cp 菌株以及分离的噬菌体。到该项目结束时，我们的目标是收集 15-20 个可组合成鸡尾酒的裂解噬菌体。该项目将加强我们对 Cp 噬菌体的生物学、多样性和治疗潜力的基本理解。由于 Cp 可以感染人类和动物，因此我们项目的成功可能代表着未来人类 Cp 噬菌体疗法的一个重要里程碑。 ****