Experimental Studies of the Structures, Energetics, and Reactions of Gas Phase Self-Assembled Biomolecular Ionic Complexes

气相自组装生物分子离子配合物的结构、能量学和反应的实验研究

基本信息

  • 批准号:
    RGPIN-2014-04429
  • 负责人:
  • 金额:
    $ 3.93万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2018
  • 资助国家:
    加拿大
  • 起止时间:
    2018-01-01 至 2019-12-31
  • 项目状态:
    已结题

项目摘要

Studies of complexes composed of metal cations and amino acids or DNA bases are important for our understanding of the physical chemistry of self-assembled biomaterials. The structures also have biological relevance in terms of, for example, telomere research. Part of the work proposed is to study the intrinsic structures, energetics, and spectroscopy of G-tetrads that compose G-quadruplexes-tracts of guanine residues at the ends of DNA strands which are an important part of the telomere. The assembley of these G-quadruplexes are typically initiated by metal cations. 9-ethylguanine (9-eG) is an ideal model for the nucleic acid as the 9-position is blocked, as it is in nucleic acids. Preliminary experimental work on (9eG)4M+ (M=Li+, Na+, K+, and Cs+) has revealed a remarkable stability of the (9eG)4Na+ complexes which theory shows us are square planar. In contrast, complexes with the other metal ions are non-planar, probably due to competition between hydrogen bonding between the nucleic acids and metal-ion/molecule interactions. Much more work is required to fully understand the balance of intermolecular forces that bind these structures. For example, what is the effect on structure stability and reactivity of metal cations with higher oxidation states such as the alkali earth, transition, and lanthanide metals. This research will also lead to valuable information on metal ion induced defects in nucleic acids. We plan to study the structures and energetics of higher order structures such as (9eG)8M+ and [(9eG)12M2]2+ of significance to biological and supramolecular materials research. The construction of these complexes relies on molecular recognition and self-assembly processes that occur in DNA, RNA, and proteins etc. Supramolecular complexes of the other nucleic acid bases as well as those composed of a mixture of bases will be studied. This research will have an impact health and cancer research, providing fundamental knowledge of the makeup of these biological complexes.*We will carry out research on polymetallic cation-peptide supramolecular assemblies. We have done preliminary CID studies on complexes such as [Ca5(GlyGly-H)8]2+ and have also begun to model them. Our current picture has the five Ca2+ ions in a plane bridged by oxygen atoms of the peptide, similar to proposed structures of naturally occurring clusters such as ferritin and the oxygen-evolving complex of photosystem II. We propose to compare the structures, energetics and reactivities of self-assembled complexes composed of different metals and larger, varied peptides in the gas phase.*These studies will be augmented by research on smaller components of the supramolecular clusters, such as metal cation-bound dimers of amino acids, DNA bases, and carbohydrates.*A new tunable IR laser, far more powerful than our current one is on order. This laser will allow us to record IRMPD spectra on G-tetrads and other strongly-bound species which was not possible with our current laser. With this new laser, our spectral range increases down to 2400 cm-1 (from 3200 cm-1) allowing us to spectroscopically characterize the fundamentally important and very intense hydrogen bonded N-H stretching bands. Besides IRMPD spectroscopy and computational techniques to determine structures of gaseous ions we will use energy-resolved CID, CID coupled with isotopic substitution, blackbody infrared radiative dissociation coupled with master equation modeling to determine binding energies, and radiative association with kinetic modeling to study bimolecular ion-molecule reactions. We are also adding simulated annealing to our repertoire of techniques to help ensure we have explored the full geometric space of our large clusters.
研究由金属阳离子和氨基酸或DNA碱基组成的配合物对于我们理解自组装生物材料的物理化学非常重要。这些结构也具有生物学相关性,例如,端粒研究。提出的部分工作是研究组成g -四联体的g -四联体的内在结构,能量学和光谱,g -四联体是DNA链末端的鸟嘌呤残基束,是端粒的重要组成部分。这些g -四联体的组合通常是由金属阳离子引发的。9-乙基鸟嘌呤(9-eG)是核酸的理想模型,因为9位被阻断,就像它在核酸中一样。对(9eG)4M+ (M=Li+, Na+, K+, Cs+)的初步实验表明,(9eG)4Na+配合物具有显著的稳定性,理论表明它是方形平面的。相比之下,与其他金属离子的配合物是非平面的,可能是由于核酸之间的氢键和金属离子/分子相互作用之间的竞争。要充分了解结合这些结构的分子间力的平衡,还需要做更多的工作。例如,对具有较高氧化态的金属阳离子(如碱土、过渡金属和镧系金属)的结构稳定性和反应性有什么影响?这项研究还将为金属离子诱导的核酸缺陷提供有价值的信息。我们计划研究对生物和超分子材料研究具有重要意义的(9eG)8M+和[(9eG)12M2]2+等高阶结构的结构和能量学。这些复合物的构建依赖于发生在DNA、RNA和蛋白质等中的分子识别和自组装过程。其他核酸碱基的超分子配合物以及由碱基混合物组成的超分子配合物将被研究。这项研究将对健康和癌症研究产生影响,为这些生物复合物的构成提供基础知识。*开展多金属阳离子-肽超分子组装研究。我们已经对[Ca5(GlyGly-H)8]2+等配合物进行了初步的CID研究,并开始对它们进行建模。我们目前的图像中有5个Ca2+离子在一个平面上,由肽的氧原子桥接,类似于光系统II的铁蛋白和光系统II的氧进化复合体等自然发生的簇的结构。我们打算比较由不同金属和较大的多肽组成的自组装配合物在气相中的结构、能量学和反应性。*这些研究将通过对超分子簇的较小组成部分的研究来加强,例如氨基酸、DNA碱基和碳水化合物的金属阳离子结合二聚体。一种新的可调谐红外激光器,比我们现在的要强大得多。该激光器将使我们能够记录g -四分体和其他强结合物种的IRMPD光谱,这是我们目前的激光器无法做到的。使用这种新型激光器,我们的光谱范围从3200 cm-1增加到2400 cm-1,使我们能够从光谱上表征基本重要且非常强烈的氢键N-H拉伸带。除了IRMPD光谱和计算技术来确定气体离子的结构外,我们还将使用能量分辨CID、CID与同位素取代耦合、黑体红外辐射解离与主方程建模耦合来确定结合能,以及辐射关联与动力学建模来研究双分子离子-分子反应。我们还将模拟退火添加到我们的技术库中,以帮助确保我们已经探索了大型集群的完整几何空间。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Fridgen, Travis其他文献

Fridgen, Travis的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Fridgen, Travis', 18)}}的其他基金

The Physical Chemistry-Structures, Energetics, and Reactions-of Self Assembled Metal Cationized Complexes in the Gas Phase
气相自组装金属阳离子配合物的物理化学——结构、能量和反应
  • 批准号:
    RGPIN-2019-05260
  • 财政年份:
    2022
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
The Physical Chemistry-Structures, Energetics, and Reactions-of Self Assembled Metal Cationized Complexes in the Gas Phase
气相自组装金属阳离子配合物的物理化学——结构、能量和反应
  • 批准号:
    RGPIN-2019-05260
  • 财政年份:
    2021
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
The Physical Chemistry-Structures, Energetics, and Reactions-of Self Assembled Metal Cationized Complexes in the Gas Phase
气相自组装金属阳离子配合物的物理化学——结构、能量和反应
  • 批准号:
    RGPIN-2019-05260
  • 财政年份:
    2020
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
The Physical Chemistry-Structures, Energetics, and Reactions-of Self Assembled Metal Cationized Complexes in the Gas Phase
气相自组装金属阳离子配合物的物理化学——结构、能量和反应
  • 批准号:
    RGPIN-2019-05260
  • 财政年份:
    2019
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
Experimental Studies of the Structures, Energetics, and Reactions of Gas Phase Self-Assembled Biomolecular Ionic Complexes
气相自组装生物分子离子配合物的结构、能量学和反应的实验研究
  • 批准号:
    RGPIN-2014-04429
  • 财政年份:
    2017
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
Experimental Studies of the Structures, Energetics, and Reactions of Gas Phase Self-Assembled Biomolecular Ionic Complexes
气相自组装生物分子离子配合物的结构、能量学和反应的实验研究
  • 批准号:
    RGPIN-2014-04429
  • 财政年份:
    2016
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
Experimental Studies of the Structures, Energetics, and Reactions of Gas Phase Self-Assembled Biomolecular Ionic Complexes
气相自组装生物分子离子配合物的结构、能量学和反应的实验研究
  • 批准号:
    RGPIN-2014-04429
  • 财政年份:
    2015
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
Experimental Studies of the Structures, Energetics, and Reactions of Gas Phase Self-Assembled Biomolecular Ionic Complexes
气相自组装生物分子离子配合物的结构、能量学和反应的实验研究
  • 批准号:
    RGPIN-2014-04429
  • 财政年份:
    2014
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
Structures, reactivities and energetics of ion-molecule complexes
离子-分子配合物的结构、反应性和能量学
  • 批准号:
    327041-2009
  • 财政年份:
    2013
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
Structures, reactivities and energetics of ion-molecule complexes
离子-分子配合物的结构、反应性和能量学
  • 批准号:
    327041-2009
  • 财政年份:
    2012
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual

相似海外基金

Experimental and numerical studies of warpage in composite structures
复合材料结构翘曲的实验和数值研究
  • 批准号:
    2883613
  • 财政年份:
    2023
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Studentship
Experimental and analytical studies on shrinkage cracking control in reinforced concrete structures using Shrinkage-Compensating Concrete (SCC)
使用补偿收缩混凝土(SCC)控制钢筋混凝土结构收缩裂缝的实验和分析研究
  • 批准号:
    550063-2020
  • 财政年份:
    2020
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Alliance Grants
Experimental and numerical studies on the leakage and patch repair performance of concrete liquid containing structures
含液混凝土结构渗漏及修补性能试验与数值研究
  • 批准号:
    530279-2018
  • 财政年份:
    2018
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Engage Grants Program
Experimental Studies of the Structures, Energetics, and Reactions of Gas Phase Self-Assembled Biomolecular Ionic Complexes
气相自组装生物分子离子配合物的结构、能量学和反应的实验研究
  • 批准号:
    RGPIN-2014-04429
  • 财政年份:
    2017
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
Theoretical studies to establish experimental methods of determination of gap structures in unconventional superconductors
建立非常规超导体间隙结构测定实验方法的理论研究
  • 批准号:
    17K05553
  • 财政年份:
    2017
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Experimental Studies of the Structures, Energetics, and Reactions of Gas Phase Self-Assembled Biomolecular Ionic Complexes
气相自组装生物分子离子配合物的结构、能量学和反应的实验研究
  • 批准号:
    RGPIN-2014-04429
  • 财政年份:
    2016
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
Studies on Statistical Inference and Experimental Designs Based on Algebraic Structures
基于代数结构的统计推断和实验设计研究
  • 批准号:
    15H06531
  • 财政年份:
    2015
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
Experimental Studies of the Structures, Energetics, and Reactions of Gas Phase Self-Assembled Biomolecular Ionic Complexes
气相自组装生物分子离子配合物的结构、能量学和反应的实验研究
  • 批准号:
    RGPIN-2014-04429
  • 财政年份:
    2015
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
Experimental Studies of the Structures, Energetics, and Reactions of Gas Phase Self-Assembled Biomolecular Ionic Complexes
气相自组装生物分子离子配合物的结构、能量学和反应的实验研究
  • 批准号:
    RGPIN-2014-04429
  • 财政年份:
    2014
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Discovery Grants Program - Individual
Experimental and Theoretical Studies toward the Development of New Corrosion-Resistant Steels and Corrosion Inhibitors for Reinforced Concrete Structures
钢筋混凝土结构新型耐腐蚀钢和缓蚀剂开发的实验和理论研究
  • 批准号:
    1435417
  • 财政年份:
    2014
  • 资助金额:
    $ 3.93万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了