Integrated physiological energetics system

这项仪器的需求来自大西洋省份的4名研究人员，他们研究水生动物如何调节新陈代谢和生理，以便在紧张的环境中茁壮成长。高温或低氧水平等环境压力因素限制了动物可获得的能量，损害了其执行关键活动的能力（例如，躲避捕食者，捕获猎物）。在特定条件下，反复暴露于亚致死应激源可以激活以牺牲非必要过程为代价提高能量可用性的途径。了解这些过程的潜在机制及其对动物的影响将使我们能够更好地预测气候变化对水生动物的影响。******此应用程序适用于最先进的孤立肌肉生理和代谢系统以及一组间歇流游泳隧道呼吸计。这些仪器将允许在多个生物尺度上测量水生动物的生理性能和有氧代谢；整个动物和孤立的肌肉水平。总之，这些技术将使研究人员能够确定代偿细胞和代谢反应如何在组织和整个动物水平上影响生理功能。******所要求的基础设施将大大提高申请人研究项目的分析能力，减轻现有设备的相当大的负担，并允许开发新的研究路线。通过这些工具进行的研究将支持培训许多高素质人员，并进一步推动加拿大在多个领域的研究工作，包括：水生动物对气候变化的反应；濒危野生大西洋鲑鱼种群的保护；头足类动物肌肉中能量有效收缩机制的适应。* * * * *

This request for instrumentation is from 4 researchers in the Atlantic Provinces who study how aquatic animals regulate their metabolism and physiology to thrive in stressful environments. Environmental stressors like high temperatures or low oxygen levels limit the amount of energy available to an animal, impairing its ability to perform critical activities (e.g. avoid predators, capture prey). Under specific conditions, repeat exposures to sub-lethal stressors can activate pathways that enhance energy availability at the expense of non-essential processes. Understanding the mechanisms underlying these processes and their consequences to the animal will allow us to better predict the impacts of climate change on aquatic fauna. ******This application is for a state of the art isolated muscle physiology and metabolism system and a bank of intermittent flow swim tunnel respirometers. These instruments will allow physiological performance and aerobic metabolism to be measured in aquatic animals at multiple biological scales; the whole animal and isolated muscle level. Together, these techniques will enable the researchers to identify how compensatory cellular and metabolic responses impact physiological function at the tissue and whole animal levels. ******The requested infrastructure would greatly enhance the analytical capacity of the applicant's research programs, relieve considerable burdens on existing equipment, and permit the development of novel lines of inquiry. The research enabled by these instruments would support the training of numerous highly qualified personnel and further Canada's research efforts in multiple fields including: the responses of aquatic animals to climate change; the conservation of imperilled wild Atlantic salmon populations; the adaptation of energetically efficient contractile mechanisms in cephalopod muscles. *****