Characterization of structurally atypical sigma factors carrying fused domains of unknown function in the plant endosymbiont, Sinorhizobium meliloti
植物内共生体苜蓿中华根瘤菌中携带未知功能融合域的结构非典型西格玛因子的表征
基本信息
- 批准号:RGPIN-2018-04583
- 负责人:
- 金额:$ 2.62万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2018
- 资助国家:加拿大
- 起止时间:2018-01-01 至 2019-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Transcription is the first major step in gene expression in all organisms. In bacteria, all gene expression is regulated by a family of general transcription factors called sigma (σ) factors. Each σ factor controls the expression of a subset (its regulon) of genes and regulons may consist of between one and thousands of genes depending on the σ factor. An important group of these proteins is comprised of Extracytoplasmic Function (ECF) σ factors and the activity of many of these is negatively regulated by a signal transduction pathway that includes an antagonistic anti-σ factor protein. ECF σ factors and their associated regulators constitute a major sensory system that, along with specific transcription factors and two-component regulatory systems, mediate the cellular ability to mount appropriate gene expression responses to changing physicochemical conditions inside and outside of the cell. ******Mechanistically, σ factors carry out two major functions: they target RNAP holoenzyme to the gene promoter and they trap the transcription start site in a single-stranded state called an open-complex. Stabilization of the open-complex is an essential step in the transcription initiation pathway and σ factor activity is partially analogous to the activity of TFIIB in archaeal and eukaryotic RNAP II. Understanding mechanistic detail of σ factor function is a very active area of research that involves a few well-characterized model systems. However, the analysis of genome sequence data has revealed the presence of structurally unusual ECF σ factor-related proteins in many bacteria and these atypical or non-canonical systems remain understudied. Several sub-groups of ECF σ factors unexpectedly carry fused protein domains of unknown or incompletely characterized function. Emerging evidence points to regulatory functions for these fused domains and the long term objective of my research program is to study mechanistic details of non-canonical transcription systems in bacteria. The study of non-canonical systems carries the promise of uncovering novel pathways to transcription initiation and unexpected physiological relationships between the regulation of gene expression and other cellular processes.*** ***Remarkably, the important endosymbiotic bacterium Sinorhizobium meliloti synthesizes two unique examples of non-canonical fused domain σ factors. This current proposal describes the study of the physiological importance of one of these proteins (RpoE9) to S. meliloti biology. To these ends, we are proposing a series of research pursuits mainly focused on discerning unexpected intersections between RpoE9 activity and other cellular regulatory and homeostatic systems. We wish to emphasize that while the study of this structurally atypical σ factor is important in itself, we also view it as a vehicle to establish and refine the study of all ECF σ factors from a more global and systems-wide perspective. *****
转录是所有生物体基因表达的第一个主要步骤。在细菌中,所有的基因表达都受到一个称为sigma(σ)因子的通用转录因子家族的调控。每个σ因子控制基因的子集(其调节子)的表达,并且取决于σ因子,调节子可以由一到数千个基因组成。这些蛋白质中的一个重要组由细胞质外功能(ECF)σ因子组成,并且这些中的许多的活性由包括拮抗性抗σ因子蛋白的信号转导途径负调节。ECF σ因子及其相关的调节因子构成了一个主要的感觉系统,其沿着特异性转录因子和双组分调节系统,介导细胞对细胞内外变化的物理化学条件进行适当基因表达反应的能力。** 从机制上讲,σ因子执行两个主要功能:它们将RNAP全酶靶向基因启动子,并将转录起始位点捕获在称为开放复合物的单链状态中。开放复合物的稳定化是转录起始途径中的重要步骤,并且σ因子活性部分类似于古细菌和真核RNAP II中的TFIIB活性。了解σ因子函数的机制细节是一个非常活跃的研究领域,涉及一些良好表征的模型系统。然而,基因组序列数据的分析已经揭示了在许多细菌中存在结构不寻常的ECF σ因子相关蛋白,并且这些非典型或非规范系统仍然未得到充分研究。ECF σ因子的几个亚组意外地携带未知或不完全表征功能的融合蛋白结构域。新出现的证据表明这些融合结构域的调控功能,我的研究计划的长期目标是研究细菌中非经典转录系统的机制细节。对非经典系统的研究有望揭示转录起始的新途径,以及基因表达调控与其他细胞过程之间意想不到的生理关系。* 值得注意的是,重要的内共生细菌苜蓿中华根瘤菌(Sinorhizobium meliloti)合成了两个独特的非典型融合结构域σ因子的例子。目前的建议描述了这些蛋白质之一(RpoE9)对S的生理重要性的研究。草蛉生物学为此,我们提出了一系列的研究追求,主要集中在辨别RpoE9活性和其他细胞调节和稳态系统之间的意想不到的交叉点。我们希望强调的是,虽然这种结构上的非典型σ因子的研究本身是重要的,我们也认为它是一个工具,建立和完善的研究,所有ECF σ因子从一个更全球和系统范围的角度。*****
项目成果
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MacLellan, Shawn其他文献
Transcription activation by the siderophore sensor Btr is mediated by ligand-dependent stimulation of promoter clearance
- DOI:
10.1093/nar/gkr1280 - 发表时间:
2012-04-01 - 期刊:
- 影响因子:14.9
- 作者:
Gaballa, Ahmed;MacLellan, Shawn;Helmann, John D. - 通讯作者:
Helmann, John D.
MacLellan, Shawn的其他文献
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{{ truncateString('MacLellan, Shawn', 18)}}的其他基金
Characterization of structurally atypical sigma factors carrying fused domains of unknown function in the plant endosymbiont, Sinorhizobium meliloti
植物内共生体苜蓿中华根瘤菌中携带未知功能融合域的结构非典型西格玛因子的表征
- 批准号:
RGPIN-2018-04583 - 财政年份:2022
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
Characterization of structurally atypical sigma factors carrying fused domains of unknown function in the plant endosymbiont, Sinorhizobium meliloti
植物内共生体苜蓿中华根瘤菌中携带未知功能融合域的结构非典型西格玛因子的表征
- 批准号:
RGPIN-2018-04583 - 财政年份:2021
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
Characterization of structurally atypical sigma factors carrying fused domains of unknown function in the plant endosymbiont, Sinorhizobium meliloti
植物内共生体苜蓿中华根瘤菌中携带未知功能融合域的结构非典型西格玛因子的表征
- 批准号:
RGPIN-2018-04583 - 财政年份:2020
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
The study of non-canonical transcriptions systems in bacteria
细菌非规范转录系统的研究
- 批准号:
386710-2010 - 财政年份:2015
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
The study of non-canonical transcriptions systems in bacteria
细菌非规范转录系统的研究
- 批准号:
386710-2010 - 财政年份:2013
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
Identification and characterization of bacteria able to genetically transform potato plant cells
能够基因转化马铃薯植物细胞的细菌的鉴定和表征
- 批准号:
461312-2013 - 财政年份:2013
- 资助金额:
$ 2.62万 - 项目类别:
Engage Grants Program
The study of non-canonical transcriptions systems in bacteria
细菌非规范转录系统的研究
- 批准号:
386710-2010 - 财政年份:2012
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
The study of non-canonical transcriptions systems in bacteria
细菌非规范转录系统的研究
- 批准号:
386710-2010 - 财政年份:2011
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
The study of non-canonical transcriptions systems in bacteria
细菌非规范转录系统的研究
- 批准号:
386710-2010 - 财政年份:2010
- 资助金额:
$ 2.62万 - 项目类别:
Discovery Grants Program - Individual
The use of a fermentation system to facilitate study of RNA polymerase holoenzyme
使用发酵系统促进 RNA 聚合酶全酶的研究
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391033-2010 - 财政年份:2009
- 资助金额:
$ 2.62万 - 项目类别:
Research Tools and Instruments - Category 1 (<$150,000)
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