Enhancing the efficacy of Pseudomonas entomophila 23S, a PGPR for biocontrol of bacterial canker disease in tomato ******

增强嗜虫假单胞菌 23S（一种用于生物防治番茄细菌性溃疡病的 PGPR）的功效 ******

• 批准号: 534001-2018
• 负责人: Smith, Donald
• 金额: $ 1.82万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=665445
• 关键词: Enhancing; efficacy; Pseudomonas; entomophila; 23S

Bacterial canker disease, caused by Clavibacter michiganensis subsp. michiganensis (Cmm) is one of the most serious diseases of tomato. The disease has caused substantial crop losses and become a serious concern for both field and greenhouse tomato production in many parts of the world, including Canada. Existing control strategies, such as seed treatment and chemical control, have shown only limited efficacy in controlling this disease. Thus, effective control strategies are urgently required. The Smith laboratory at McGill University has discovered several strains that show antagonistic activity against Cmm, and an anti-Cmm compound produced by one of these strains, Pseudomonas sp. 23S was isolated. Given the seriousness of the disease damages, this anti-Cmm compound, as well as the producing stain is great potential as a new effective control strategy for tomato bacterial canker. Lallemand has been interested in developing a product to control Cmm and has been looking for potential approaches. With Lallemand's expertise on strain production, scale-up, formulation and product development, success in the proposed work would allow a full effort to completely understand the potential of a technology based on Pseudomonas sp. 23S and eventual full deployment in the Canadian context. We propose to study the anti- Cmm compounds produced by Pseudomonas sp. 23S and to determine the best production method. The project will consist of elucidating the minimum inhibitory concentration of the anti-Cmm compound, studying the concentration of the compound during various incubation phases, and examining the effects of Cmm on the production of anti- Cmm compounds produced by Pseudomonas sp. 23S. This work will improve the sustainability and economics of Canadian tomato producers and give them further leadership in the North American tomato production market. The work will provide a deeper understanding of the newly discovered anti-Cmm compound produced by Pseudomonas sp. 23S and its best production method, leading to a broader collaboration between Lallemand and McGill University; one that will allow further utilization of biologicals and the development of new products in agriculture.

细菌性溃疡病，由密歇根棒状杆菌亚种引起。密歇根病（Cmm）是番茄最严重的病害之一。该疾病已造成大量农作物损失，并成为包括加拿大在内的世界许多地区田间和温室番茄生产的严重问题。现有的控制策略，例如种子处理和化学控制，在控制这种疾病方面效果有限。因此，迫切需要有效的控制策略。麦吉尔大学史密斯实验室发现了几种对 Cmm 显示拮抗活性的菌株，以及其中一种菌株假单胞菌产生的抗 Cmm 化合物。 23S被隔离。考虑到该病害的严重性，这种抗 Cmm 化合物以及产生的染色剂具有作为番茄细菌性溃疡病新的有效控制策略的巨大潜力。 Lallemand 一直对开发控制 Cmm 的产品感兴趣，并一直在寻找潜在的方法。凭借拉勒曼在菌株生产、放大、配方和产品开发方面的专业知识，拟议工作的成功将有助于充分了解基于假单胞菌的技术的潜力。 23S 并最终在加拿大全面部署。我们建议研究假单胞菌产生的抗 Cmm 化合物。 23S并确定最佳生产方法。该项目将包括阐明抗 Cmm 化合物的最低抑制浓度、研究该化合物在不同培养阶段的浓度以及检查 Cmm 对假单胞菌产生的抗 Cmm 化合物的影响。 23S。这项工作将提高加拿大番茄生产商的可持续性和经济效益，并使他们在北美番茄生产市场上进一步处于领先地位。这项工作将使人们更深入地了解新发现的由假单胞菌产生的抗 Cmm 化合物。 23S 及其最佳生产方法，促成拉勒曼德大学和麦吉尔大学之间更广泛的合作；它将允许进一​​步利用生物制品和开发农业新产品。