Genetic enhancement of cellulose production by Consenzeae myxofaciens

Consenzeae myxofaciens 纤维素生产的遗传增强

• 批准号: 538044-2018
• 负责人: Szatmari, George
• 金额: $ 0.36万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Engage Plus Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=666331
• 关键词: Genetic; enhancement; cellulose; production; Consenzeae

Cosenzeae myxofaciens, also referred to as Proteus myxofaciens is a Gram-negative bacterium in the same**family as species such as P.mirabilis. This strain is industrially important for its ability to produce large**quantities of biocellulose. Biocellulose displays properties different from plant-based cellulose, notably its**long fiber length and unique composition, making it particularly useful in applications in the biomedical,**electronic and food science fields. Bacterial biocellulose has been traditionally produced by bacteria such as**Gluconeobactobacter xylinus, a bacterium with an absolute requirement for oxygen in order to grow. However,**C.myxofaciens is a facultative microbe, able to grow in the presence or absence of oxygen, making the strain**much more useful to industry. Our laboratory has begun to develop genetic tools to modify this organism with**the ultimate goal of making the bacterium less of a potential pathogen, with increased and more stable**biocellulose production. These tools include the creation of a library of transposon insertions, and methods of**effectively introducing foreign DNA into C.myxofaciens in order to specifically inactivate genes by specifically**deleting them (markerless deletion). The aim of this proposal is to use these technologies to create genetically**modified C.myxofaciens strains that are safer and are able produce biocellulose at reduced cost and at a greater**yield.

Cosenzeae myxofaciens，也称为Proteus myxofaciens，是一种革兰氏阴性细菌，与P.mirabilis等物种属于同一科。该菌株由于其能够产生大量生物纤维素而在工业上是重要的。生物纤维素显示出与植物纤维素不同的特性，特别是其 ** 长纤维长度和独特的组成，使其在生物医学，** 电子和食品科学领域的应用中特别有用。传统上，细菌生物纤维素是由诸如木葡糖酸杆菌（Gluconeobactobacter xylinus）的细菌产生的，木葡糖酸杆菌是一种绝对需要氧气才能生长的细菌。然而，**C.myxofaciens是一种兼性微生物，能够在存在或不存在氧气的情况下生长，使菌株 ** 对工业更有用。我们的实验室已经开始开发遗传工具来修改这种生物体，最终目标是使细菌不那么成为潜在的病原体，增加和更稳定的生物纤维素生产。这些工具包括转座子插入文库的创建，以及将外源DNA有效引入粘梭菌中以通过特异性缺失它们（无标记缺失）来特异性地修饰基因的方法。该提案的目的是利用这些技术来创建更安全的基因修饰C.myxofaciens菌株，并且能够以更低的成本和更高的产量生产生物纤维素。