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Differentiation of equine induced pluripotent stem cells into a keratinocyte lineage

马诱导多能干细胞分化为角质形成细胞谱系

基本信息

批准号：
250231-2012
负责人：
Theoret, Christine
金额：
$ 1.75万
依托单位：
Université de Montréal
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2018
资助国家：
加拿大
起止时间：
2018-01-01 至 2019-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=666706
关键词：
Differentiation equine induced pluripotent stem

项目摘要

Impaired wound healing is a common problem in both human and veterinary patients. In the horse, chronic non-healing wounds pose a welfare implication and bear a significant financial impact on the equine industry. A critical component of chronic non-healing wounds is the absence of an epithelial cover (epidermis).**Advances in skin grafting technology, based on the transplantation of adult stem cells, represent a novel approach to treating chronic wounds. Unfortunately, the resultant skin substitute lacks hair follicles and sweat glands. Embryonic stem cells, on the other hand, are able to commit to many different cell types, which may allow the generation of more complete grafts. However, ethical and technical problems preclude realizing the full potential of embryonic cells. **The recent discovery that adult cells can be reprogrammed to an embryonic-like state presents enormous potential for tissue repair and regeneration. Indeed, these induced pluripotent cells can transform into keratinocytes and thus establish a fully functional epidermis, at least in the mouse. **This study aims to develop a method to force induced pluripotent stem cells of horses to commit to a fully functional skin keratinocyte and to use these cells in an effort to reconstitute a normal epidermal cover when grafted onto wounds of horses. **Advances achieved in the horse wound model may aid the validation of stem cell-based therapies required prior to moving into the human clinical setting.**

伤口愈合受损是人类和兽医患者的常见问题。对于马来说，慢性不愈合的伤口会带来福利影响，并对马业产生重大的财务影响。慢性不愈合伤口的一个关键组成部分是缺乏上皮覆盖层（表皮）。 **基于成体干细胞移植的皮肤移植技术的进步代表了治疗慢性伤口的新方法。不幸的是，由此产生的皮肤替代品缺乏毛囊和汗腺。另一方面，胚胎干细胞能够分化成许多不同的细胞类型，这可能会产生更完整的移植物。然而，伦理和技术问题阻碍了胚胎细胞的全部潜力的发挥。 **最近发现成体细胞可以被重新编程为胚胎样状态，这为组织修复和再生提供了巨大的潜力。事实上，这些诱导的多能细胞可以转化为角质形成细胞，从而建立功能齐全的表皮，至少在小鼠中是这样。 **本研究旨在开发一种方法，迫使马的诱导多能干细胞分化为功能齐全的皮肤角质形成细胞，并在移植到马的伤口上时使用这些细胞来重建正常的表皮覆盖层。 **在马伤口模型中取得的进展可能有助于验证进入人类临床环境之前所需的基于干细胞的疗法。**