Production of bioplastics using pulp and paper mill activated sludge (PPMAS) as a raw material and new sludge isolated microorganisms

以制浆造纸厂活性污泥（PPMAS）为原料和新型污泥分离微生物生产生物塑料

• 批准号: 514366-2017
• 负责人: Tyagi, RajeshwarDayal
• 金额: $ 11.66万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=667338
• 关键词: Production; bioplastics; using; pulp; paper

Wastewater sludge is produced in large quantity and its safe disposal in the environment is a worldwide problem. The long-term goal of our research is to produce value-added marketable products using wastewater sludge as a raw material. On the other hand, demand for bioplastics or polyhydroxy alkanoates (PHA) has increased significantly due to environmental problems associated with conventional plastics, necessity to reduce greenhouse gases (GHG) and the development of government measures to minimize the use of synthetic plastics. Therefore, the principal objective of the present research is to develop an economical process for bioplastics production using pulp and paper mill activated sludge (PPMAS) and other waste material as raw materials. In fact, we have obtained a consortium of sludge microorganisms, which accumulates PHA up to 70% w/w. The specific objectives of the present research project include isolation and identification of individual highly active strains from the consortium, process optimization studies in shake flasks and fermenters to maximize the PHA production employing the isolated strains and the standard strains as control with pre-treated or non-treated PPMAS as a raw material. The PPMAS will also be tested alone or fortified with extra carbon source (crude glycerol) to increase the PHA productivity. The successful completion of the research will: 1) provide a new process for low cost bioplastics; 2) reduce the sludge volume by 60-70 % w/w and use of crude glycerol produced during biodiesel production; and 3) minimize production of greenhouse gases through use of waste carbon, which otherwise must be disposed safely. Furthermore, this research is very timely and will aid Canadian industry to develop eco-friendly, commercially viable PHA at very competitive cost strengthening Canada's economy. 3 Ph.D, 1 PDF and a research associate will be trained on this new subject.

废水污泥产生量大，其在环境中的安全处置是一个世界性难题。我们研究的长期目标是利用废水污泥作为原料生产出具有附加值的适销产品。另一方面，由于与传统塑料相关的环境问题，减少温室气体（GHG）的必要性以及政府采取措施尽量减少合成塑料的使用，对生物塑料或聚羟基链烷酸酯（PHA）的需求显着增加。因此，本研究的主要目标是开发一种经济的工艺，用于生物塑料的生产使用纸浆和造纸厂活性污泥（PPMAS）和其他废物材料作为原料。事实上，我们已经获得了污泥微生物的聚生体，其积累PHA高达70%w/w。本研究项目的具体目标包括从该财团中分离和鉴定单个高活性菌株，在摇瓶和发酵罐中进行工艺优化研究，以最大限度地提高PHA生产，使用分离的菌株和标准菌株作为对照，以预处理或未处理的PPMAS为原料。还将单独测试PPMAS或用额外碳源（粗甘油）强化PPMAS以提高PHA生产率。该研究的成功完成将：1）提供一种低成本生物塑料的新工艺; 2）将污泥体积减少60- 70%w/w，并减少生物柴油生产过程中产生的粗甘油的使用; 3）通过使用废碳最大限度地减少温室气体的产生，否则必须安全处理。此外，这项研究是非常及时的，将有助于加拿大工业发展生态友好，商业上可行的PHA在非常有竞争力的成本加强加拿大的经济。3名博士，1名PDF和一名研究助理将接受这一新课题的培训。