Ultracentrifugation Facility

超速离心设备

• 批准号: RTI-2019-00109
• 负责人: Zamble, Deborah
• 金额: $ 7.5万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=669549
• 关键词: Ultracentrifugation; Facility

High-speed ultracentrifugation is essential for diverse areas of modern chemical research. This multi-purpose instrument is required by many labs in our department, particularly those working in the areas of biological and materials chemistry. For example, ultracentrifugation is essential for the isolation and characterization of membrane proteins, as well as for reconstitution of these multiprotein complexes into artificial membrane systems, which is necessary to investigate the functions and mechanisms of action of these critical biological molecules. Similarly, the ability to process samples in a high-speed centrifuge is critical for the purification and characterization of new nanomaterials, such as nanoparticles for sensitive, multiplexed biomarker detection. ******The equipment requested delivers the high-speed centrifugation required, and supports membrane isolation, cellular fractionation, nanoparticle purification and analysis, as well as separation of subcellular particles and macromolecules, highlighting its central position as core infrastructure for our research programs. We currently do not have such capabilities.******Combined, in the groups of the applicants we are currently mentoring 35 students and post-doctoral fellows, and all of these trainees will be trained on and have access to this key piece of infrastructure. In addition, this equipment will be located in a departmental facility, so all trainees in the department will have access to training and operation of this infrastructure. This type of instrument is commonly used in the biotechnology, materials science, and pharmaceutical industries, so hands-on training and method development is beneficial to trainees seeking future employment in these areas.  *****

高速超速离心对于现代化学研究的各个领域都是必不可少的。这台多功能仪器是我们系的许多实验室，特别是生物和材料化学领域的实验室所需要的。例如，超速离心法对于膜蛋白的分离和鉴定以及将这些多蛋白复合体重组为人工膜系统是必不可少的，这对于研究这些关键生物分子的功能和作用机制是必要的。同样，在高速离心机中处理样品的能力对于新纳米材料的提纯和表征至关重要，例如用于灵敏、多路生物标志物检测的纳米颗粒。*所需的设备提供所需的高速离心机，并支持膜分离、细胞分离、纳米颗粒纯化和分析，以及亚细胞颗粒和大分子的分离，突出了其作为我们研究计划核心基础设施的中心地位。我们目前没有这样的能力。*加起来，我们目前正在指导35名学生和博士后研究员的申请者小组，所有这些受训人员都将接受这一关键基础设施的培训和使用。此外，这些设备将设在部门的设施中，因此部门的所有受训人员都将能够获得这一基础设施的培训和操作。这种类型的仪器通常用于生物技术、材料科学和制药行业，因此动手培训和方法开发有利于受训者在这些领域寻找未来的就业机会。*****