Controlled Atmospheric Environment for Cell Growth and Manipulation

这个星球上的许多生物生活在微有氧或厌氧环境下，比如在人类的肠道中，它们暴露在各种气体中，这些气体决定了细胞的新陈代谢。因此，在可控和恒定的环境下培养和操作细胞培养的能力对于理解这些系统的作用机制至关重要。Zamble实验室需要受控的环境条件来研究几种人类病原体（如幽门螺杆菌和大肠杆菌）中独特的金属代谢。尼茨实验室正在开发基于碲的探针，以研究肿瘤组织中的缺氧，这是另一种氧气可用性降低的系统，并将独特的标签放入蛋白质中。所要求的设备提供所需气体环境的广度，并支持培养生长，操作和分析，突出其作为我们研究计划核心基础设施的中心地位。我们目前没有这样的能力。

Many organisms on this planet live under micro-aerobic or anaerobic environments, such as in the human gut, where they are exposed to a variety gases that dictate cellular metabolism. As such, the ability to grow and manipulate cell cultures under a controlled and constant environment is essential to understanding the mechanisms of action of these systems. The Zamble lab requires controlled-environment conditions to study the unique metal metabolism in several human pathogens such as Helicobacter pylori and Escherichia coli. The Nitz lab is developing tellurium based probes to study hypoxia in tumor tissue, another system with reduced oxygen availability, and to put unique labels into proteins. The equipment requested delivers the breadth of gaseous environments required, and supports culture growth, manipulation, and analysis, highlighting its central position as core infrastructure for our research programs. We currently do not have such capabilities. Combined, in our groups we are currently mentoring 15 students and post-doctoral fellows, and all of these trainees will be trained on, and have access to, this key piece of infrastructure. Furthermore, this type of instrument is commonly used in the biotechnology, biological, and pharmaceutical industries, so hands-on training and method development is beneficial to students seeking future employment in these areas.