Controlled Atmospheric Environment for Cell Growth and Manipulation

用于细胞生长和操作的受控大气环境

• 批准号: RTI-2017-00738
• 负责人: Zamble, Deborah
• 金额: $ 6.8万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=616756
• 关键词: Controlled; Atmospheric; Environment; Cell; Growth

Many organisms on this planet live under micro-aerobic or anaerobic environments, such as in the human gut, where they are exposed to a variety gases that dictate cellular metabolism. As such, the ability to grow and manipulate cell cultures under a controlled and constant environment is essential to understanding the mechanisms of action of these systems. The Zamble lab requires controlled-environment conditions to study the unique metal metabolism in several human pathogens such as Helicobacter pylori and Escherichia coli. The Nitz lab is developing tellurium based probes to study hypoxia in tumor tissue, another system with reduced oxygen availability, and to put unique labels into proteins. The equipment requested delivers the breadth of gaseous environments required, and supports culture growth, manipulation, and analysis, highlighting its central position as core infrastructure for our research programs. We currently do not have such capabilities. Combined, in our groups we are currently mentoring 15 students and post-doctoral fellows, and all of these trainees will be trained on, and have access to, this key piece of infrastructure. Furthermore, this type of instrument is commonly used in the biotechnology, biological, and pharmaceutical industries, so hands-on training and method development is beneficial to students seeking future employment in these areas.

这个星球上的许多生物体生活在微需氧或厌氧环境下，例如在人类肠道中，它们暴露于各种气体中，这些气体决定了细胞代谢。因此，在受控和恒定的环境下生长和操纵细胞培养物的能力对于理解这些系统的作用机制至关重要。Zamble实验室需要受控环境条件来研究几种人类病原体（如幽门螺杆菌和大肠杆菌）中独特的金属代谢。Nitz实验室正在开发基于碲的探针，以研究肿瘤组织中的缺氧，这是另一个氧气可用性降低的系统，并将独特的标签放入蛋白质中。所要求的设备提供了所需的气体环境的广度，并支持文化的增长，操纵和分析，突出了其作为我们的研究计划的核心基础设施的中心地位。我们目前没有这样的能力。 在我们的小组中，我们目前正在指导15名学生和博士后研究员，所有这些受训人员都将接受这一关键基础设施的培训。此外，这种类型的仪器通常用于生物技术，生物和制药行业，因此实践培训和方法开发有利于学生在这些领域寻求未来就业。