Novel Biogeochemical Pathways, Patterns and Measurements of Nitrogen and Phosphorus in Lakes and Reservoirs

湖泊和水库中氮和磷的新生物地球化学途径、模式和测量

• 批准号: RGPIN-2017-06457
• 负责人: Hudson, Jeff
• 金额: $ 1.75万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=679890
• 关键词: Novel; Biogeochemical; Pathways; Patterns; Measurements

This proposal examines the abiotic and biotic factors, including human, that affect biogeochemical processes and water quality in aquatic ecosystems. The factors giving rise to cyanobacterial blooms and their toxins are under intense research. Concern over the loss of ecosystem services (e.g., water quality) has been a major focus. However, only recently has the effect of cyanobacteria on ecosystem function been considered. Cyanobacteria have widespread impacts on food webs through toxin production, resistance to grazing and as poor food quality for grazers. Hence, when lakes are dominated by cyanobacteria their planktonic food webs should be functioning inefficiently. I will test this hypothesis with novel techniques that directly measure food web turnover efficiency. My next objective is to compare two important biogeochemical pathways of P in Lake Diefenbaker (SK). Loading of P from rivers into reservoirs is often a major determinant of water quality. Similarly, planktonic P regeneration is known to be important to maintain productivity. However, the importance of each pathway relative to the other is unknown, despite the potential significance of these processes for lake and reservoir management. Therefore, I propose to measure and compare both pathways in Lake Diefenbaker. My third objective is to determine whole-lake rates of photoammonification. This is a process by which dissolved organic nitrogen (DON) is photodecomposed to the bioavailable nutrient NH4 in waterbodies. My preliminary research suggests that this process may be significant in spring and summer. However, the rate at which DON is converted to NH4 as a function of depth in lakes has not been determined, a necessary step to estimate whole system rates. Therefore, I will measure depth integrated photoammonification in a diverse set of prairie and Precambrian Shield lakes in order to establish the relevance of this pathway. My next objective concerns the measurement of NO3, an important nutrient affecting water quality in lakes. NO3 is usually measured with colorimetric methods. However, colorimetric methods entail many steps and are prone to interferences. The second derivative UV technique for NO3 measurement is emerging as an alternative method; it is rapid, less prone to interferences, and generates less waste. However, this technique has not been tested in more challenging waters typical of prairie regions (e.g., of greater conductivity and higher dissolved organic carbon concentration). My preliminary analyses indicate that both techniques differ greatly in estimating NO3 in such waters. Therefore, I will compare their performance and determine if the second derivative technique is suitable for NO3 measurement in prairie waterbodies. These studies will advance our understanding of biogeochemical processes in lakes and reservoirs, and ultimately aid in the management and protection of inland waters.

该提案审查了影响水生生态系统中生物地球化学过程和水质的非生物和生物因素，包括人类因素。引起蓝藻水华的因素及其毒素正在紧张的研究中。对生态系统服务丧失的关切（例如，水质）一直是一个主要焦点。然而，直到最近才考虑到蓝藻对生态系统功能的影响。蓝细菌通过产生毒素、抵抗放牧和作为食草动物的劣质食物对食物网产生广泛影响。 因此，当湖泊被蓝藻所控制时，它们的浮游食物网应该是低效的。我将测试这个假设与新的技术，直接测量食物网周转效率。我的下一个目标是比较两个重要的磷在迪芬贝克湖（SK）的地球化学途径。从河流到水库的磷负荷通常是水质的主要决定因素。类似地，已知磷素营养的再生对于维持生产力是重要的。然而，每个路径相对于其他的重要性是未知的，尽管这些过程的湖泊和水库管理的潜在意义。因此，我建议在迪芬贝克湖测量和比较这两种途径。我的第三个目标是确定整个湖泊的光氨化率。这是一个溶解有机氮（DON）在水体中被光分解为生物可利用营养素NH 4的过程。我的初步研究表明，这一过程在春季和夏季可能很重要。然而，DON转化为NH 4的速率作为湖泊深度的函数尚未确定，这是估计整个系统速率的必要步骤。因此，我将测量深度综合光氨化作用在一组不同的草原和前寒武纪盾湖，以建立这条途径的相关性。我的下一个目标是测量NO3，这是一种影响湖泊水质的重要营养物质。NO3通常用比色法测量。然而，比色法需要许多步骤并且易于受到干扰。NO3测量的二阶导数UV技术正在成为一种替代方法;它快速，不易受干扰，产生的废物较少。然而，这种技术还没有在草原地区典型的更具挑战性的沃茨中进行测试（例如，具有更大的电导率和更高的溶解有机碳浓度）。我的初步分析表明，这两种技术在估计这样的沃茨中的NO3时有很大的不同。因此，我将比较它们的性能，并确定二阶导数技术是否适用于草原水体中的NO3测量。 这些研究将促进我们对湖泊和水库中的地球化学过程的理解，并最终有助于内陆沃茨的管理和保护。