Surface Modification of Graphene Oxide for Detecting Oligonucleotide

Grafoid Inc. （Grafoid）专注于碳基二维纳米结构的研究和开发，如石墨烯，氧化石墨烯。最近，Grafoid公司开发了一种大规模的氧化石墨烯工艺。氧化石墨烯（Graphene oxide， GO）是由碳呈六角形排列而成的二维（2D）晶体结构，其表面有各种氧官能团，通过适当的表面修饰，使氧化石墨烯产品具有各种性能。此外，氧化石墨烯具有很强的荧光猝灭能力，在生物分子检测方面也显示出潜力。在这里，我们将使用Grafoid对氧化石墨烯进行表面改性和表征。荧光特性作为目标单链DNA序列的函数将被研究。张博士与Grafoid的合作将有助于公司将新的氧化石墨烯产品推向市场，并及时建立高效的产品管道。参与该项目的学员将进一步发展他们在荧光技术和纳米生物技术方面的知识，并获得设计和开发生物传感器的实际技能。本次合作项目的成功成果将有助于我们深刻理解氧化石墨烯生物偶联对DNA传感器性能的影响，从而对加拿大二维产品和DNA检测产生重大影响。

Grafoid Inc. (Grafoid) focuses on the research and development of carbon-based two dimensional nanostructures, e.g. graphene, graphene oxide. Recently, Grafoid has developed a large-scale process of graphene oxide. Graphene oxide (GO) is a two-dimensional (2D) crystal structure made of carbon arranged in a hexagonal pattern with various oxygen functional groups on the surface which makes GO products have various properties through suitable surface modification. In addition, GO has shown the potential for biomolecule detection due to its strong capability for fluorescence quenching. Here, we will work with Grafoid to the surface modification and characterization of GO. The fluorescence properties as a function of the target single stranded DNA sequences will be investigated. The collaboration between Dr. Zhang and Grafoid will help the company move their new graphene oxide products to the market, and build an efficient product pipeline timely. Trainees involved in this project will further develop their knowledge on fluorescence technique and nanobiotechnology, and gain hands-on skills on the design and development of biosensors. The successful outcome of this collaborative project will help us have a profound understanding of the effect of bioconjugation of graphene oxide on the performance of DNA sensor, and, therefore, have a significant impact on Canada's two-dimensional products and DNA detection.