Surface Modification of Graphene Oxide for Detecting Oligonucleotide

Grafoid Inc.(Grafoid)专注于碳基二维纳米结构的研究和开发，例如石墨烯、石墨烯氧化物。最近，Grafoid开发了大规模的氧化石墨烯工艺。氧化石墨烯(GO)是一种由碳原子组成的二维(2D)晶体结构，表面具有多种含氧官能团，通过适当的表面修饰使GO产物具有多种性能。此外，由于GO具有很强的荧光猝灭能力，因此在生物分子检测方面显示出很大的潜力。在这里，我们将与Grafoid合作对GO进行表面修饰和表征。作为目标单链DNA序列的函数的荧光特性将被研究。张博士和Grafoid的合作将帮助公司将他们新的氧化石墨烯产品推向市场，并及时建立高效的产品线。参与该项目的学员将进一步发展他们在荧光技术和纳米生物技术方面的知识，并获得设计和开发生物传感器的实践技能。这一合作项目的成功将有助于我们深刻理解氧化石墨烯生物偶联对DNA传感器性能的影响，从而对加拿大的二维产品和DNA检测产生重大影响。

Grafoid Inc. (Grafoid) focuses on the research and development of carbon-based two dimensional nanostructures, e.g. graphene, graphene oxide. Recently, Grafoid has developed a large-scale process of graphene oxide. Graphene oxide (GO) is a two-dimensional (2D) crystal structure made of carbon arranged in a hexagonal pattern with various oxygen functional groups on the surface which makes GO products have various properties through suitable surface modification. In addition, GO has shown the potential for biomolecule detection due to its strong capability for fluorescence quenching. Here, we will work with Grafoid to the surface modification and characterization of GO. The fluorescence properties as a function of the target single stranded DNA sequences will be investigated. The collaboration between Dr. Zhang and Grafoid will help the company move their new graphene oxide products to the market, and build an efficient product pipeline timely. Trainees involved in this project will further develop their knowledge on fluorescence technique and nanobiotechnology, and gain hands-on skills on the design and development of biosensors. The successful outcome of this collaborative project will help us have a profound understanding of the effect of bioconjugation of graphene oxide on the performance of DNA sensor, and, therefore, have a significant impact on Canada's two-dimensional products and DNA detection.