Transcriptional complexity at the Hoxa5 locus: Characterization of the role of the Hoxa5-associated long noncoding RNAs
Hoxa5 位点的转录复杂性:Hoxa5 相关长非编码 RNA 作用的表征
基本信息
- 批准号:RGPIN-2015-05055
- 负责人:
- 金额:$ 2.19万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2019
- 资助国家:加拿大
- 起止时间:2019-01-01 至 2020-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The development of a multicellular organism from the fertilized egg is a complex process involving numerous genes expressed precisely in space and time. Hox genes encode proteins that control the expression of other genes. They occupy a key position in the genetic hierarchy dictating the formation of the embryo. Mutations in Hox genes result in a panoply of anomalies affecting the embryo and causing diseases in the adult, pinpointing the large range of action of Hox genes throughout life. A complex array of different modes of regulation governs the specific expression of Hox genes indicative of the importance of correct Hox expression for the normal development of the organism. One of these modes involves the long noncoding (lnc) RNAs found throughout the genome and present along the Hox gene clusters. Our laboratory is interested in elucidating the molecular mechanisms responsible for the spatio-temporal pattern of Hox gene expression during mammalian development. We use the Hoxa5 gene as a model. We have shown that Hoxa5 is essential for the formation of several structures including the skeleton, the lung, the gut, the ovary, and the thyroid and mammary glands, revealing the crucial role of Hoxa5 for normal development. Moreover, we have discovered that the Hoxa5 specific regional expression in the embryo is under the control of several DNA sequences and transcriptional factors. Multiple RNAs containing Hoxa5 sequences are produced but only one encodes for the HOXA5 protein. The other Hoxa5-associated lncRNAs possess a developmentally-regulated expression suggesting that they are also involved in embryo formation. However, their exact function and how they are integrated in the developmental program remain unknown. Our working hypothesis is that the Hoxa5-associated lncRNAs are important for the coordinated spatio-temporal expression of the Hox genes during embryogenesis and they also contribute to the control of large-scale gene expression programs involved in development. We will pursue our study of the control mechanisms of Hoxa5 expression by analyzing the role played by Hoxa5-associated lncRNAs during mouse embryogenesis. This will be addressed by using molecular and genetic approaches. Our research program will lead to a better understanding of the precise regulation of Hox expression, which is essential for Hox gene function and hence for the correct embryo development.**
从受精卵发育成多细胞生物体是一个复杂的过程,涉及大量在空间和时间上精确表达的基因。HOX基因编码控制其他基因表达的蛋白质。它们在决定胚胎形成的遗传层次结构中占有关键地位。HOX基因的突变导致了一系列影响胚胎并导致成人疾病的异常,准确地指出了HOX基因在一生中的大范围作用。一系列复杂的不同调控模式调控着HOX基因的特定表达,表明正确的HOX表达对生物体正常发育的重要性。其中一种模式涉及在基因组中发现的、沿Hox基因簇存在的长非编码(LNC)RNA。我们的实验室致力于阐明哺乳动物发育过程中HOX基因表达的时空模式的分子机制。我们使用Hoxa5基因作为模型。我们已经证明了Hoxa5对包括骨骼、肺、肠道、卵巢、甲状腺和乳腺在内的几种结构的形成是必不可少的,揭示了Hoxa5对正常发育的关键作用。此外,我们还发现Hoxa5在胚胎中的特异性区域表达受几个DNA序列和转录因子的控制。产生了多个包含Hoxa5序列的RNA,但只有一个编码HOXA5蛋白。其他与Hoxa5相关的lncRNA具有发育调节的表达,这表明它们也参与了胚胎的形成。然而,它们的确切功能以及它们如何整合到发展计划中仍不清楚。我们的工作假设是,与Hoxa5相关的lncRNAs在胚胎发育过程中对于HOX基因的时空协调表达是重要的,它们也有助于调控参与发育的大规模基因表达程序。我们将通过分析Hoxa5相关的lncRNAs在小鼠胚胎发育过程中所起的作用来进一步研究Hoxa5表达的调控机制。这将通过使用分子和遗传方法来解决。我们的研究计划将使我们更好地了解HOX表达的精确调控,这对HOX基因的功能至关重要,从而对正确的胚胎发育至关重要。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Jeannotte, Lucie其他文献
HOXA5 plays tissue-specific roles in the developing respiratory system
- DOI:
10.1242/dev.152686 - 发表时间:
2017-10-01 - 期刊:
- 影响因子:4.6
- 作者:
Landry-Truchon, Kim;Houde, Nicolas;Jeannotte, Lucie - 通讯作者:
Jeannotte, Lucie
Sustained Hox5 gene activity is required for respiratory motor neuron development.
- DOI:
10.1038/nn.3242 - 发表时间:
2012-12 - 期刊:
- 影响因子:25
- 作者:
Philippidou, Polyxeni;Walsh, Carolyn M.;Aubin, Josee;Jeannotte, Lucie;Dasen, Jeremy S. - 通讯作者:
Dasen, Jeremy S.
Partial functional redundancy between Hoxa5 and Hoxb5 paralog genes during lung morphogenesis
- DOI:
10.1152/ajplung.00006.2013 - 发表时间:
2013-06-01 - 期刊:
- 影响因子:4.9
- 作者:
Boucherat, Olivier;Montaron, Severine;Jeannotte, Lucie - 通讯作者:
Jeannotte, Lucie
Impact of the loss of Hoxa5 function on lung alveogenesis
- DOI:
10.2353/ajpath.2006.051333 - 发表时间:
2006-10-01 - 期刊:
- 影响因子:6
- 作者:
Mandeville, Isabel;Aubin, Josee;Jeannotte, Lucie - 通讯作者:
Jeannotte, Lucie
Crucial requirement of ERK/MAPK signaling in respiratory tract development
- DOI:
10.1242/dev.110254 - 发表时间:
2014-08-01 - 期刊:
- 影响因子:4.6
- 作者:
Boucherat, Olivier;Nadeau, Valerie;Jeannotte, Lucie - 通讯作者:
Jeannotte, Lucie
Jeannotte, Lucie的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Jeannotte, Lucie', 18)}}的其他基金
Understanding how the master transcriptional regulator HOXA5 defines cell fate in the developing embryo
了解主转录调节因子 HOXA5 如何定义发育中胚胎的细胞命运
- 批准号:
RGPIN-2020-06365 - 财政年份:2022
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Understanding how the master transcriptional regulator HOXA5 defines cell fate in the developing embryo
了解主转录调节因子 HOXA5 如何定义发育中胚胎的细胞命运
- 批准号:
RGPIN-2020-06365 - 财政年份:2021
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Understanding how the master transcriptional regulator HOXA5 defines cell fate in the developing embryo
了解主转录调节因子 HOXA5 如何定义发育中胚胎的细胞命运
- 批准号:
RGPIN-2020-06365 - 财政年份:2020
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Transcriptional complexity at the Hoxa5 locus: Characterization of the role of the Hoxa5-associated long noncoding RNAs
Hoxa5 位点的转录复杂性:Hoxa5 相关长非编码 RNA 作用的表征
- 批准号:
RGPIN-2015-05055 - 财政年份:2018
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Transcriptional complexity at the Hoxa5 locus: Characterization of the role of the Hoxa5-associated long noncoding RNAs
Hoxa5 位点的转录复杂性:Hoxa5 相关长非编码 RNA 作用的表征
- 批准号:
RGPIN-2015-05055 - 财政年份:2017
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Transcriptional complexity at the Hoxa5 locus: Characterization of the role of the Hoxa5-associated long noncoding RNAs
Hoxa5 位点的转录复杂性:Hoxa5 相关长非编码 RNA 作用的表征
- 批准号:
RGPIN-2015-05055 - 财政年份:2016
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Transcriptional complexity at the Hoxa5 locus: Characterization of the role of the Hoxa5-associated long noncoding RNAs
Hoxa5 位点的转录复杂性:Hoxa5 相关长非编码 RNA 作用的表征
- 批准号:
RGPIN-2015-05055 - 财政年份:2015
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Transcriptional complexity at the Hoxa5 locus
Hoxa5 位点的转录复杂性
- 批准号:
194559-2010 - 财政年份:2014
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Transcriptional complexity at the Hoxa5 locus
Hoxa5 位点的转录复杂性
- 批准号:
194559-2010 - 财政年份:2013
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
Transcriptional complexity at the Hoxa5 locus
Hoxa5 位点的转录复杂性
- 批准号:
194559-2010 - 财政年份:2012
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Grants Program - Individual
相似海外基金
Conference: 17th International Conference on Computability, Complexity and Randomness (CCR 2024)
会议:第十七届可计算性、复杂性和随机性国际会议(CCR 2024)
- 批准号:
2404023 - 财政年份:2024
- 资助金额:
$ 2.19万 - 项目类别:
Standard Grant
Addressing the complexity of future power system dynamic behaviour
解决未来电力系统动态行为的复杂性
- 批准号:
MR/S034420/2 - 财政年份:2024
- 资助金额:
$ 2.19万 - 项目类别:
Fellowship
Addressing the complexity of future power system dynamic behaviour
解决未来电力系统动态行为的复杂性
- 批准号:
MR/Y00390X/1 - 财政年份:2024
- 资助金额:
$ 2.19万 - 项目类别:
Fellowship
CAREER: Complexity Theory of Quantum States: A Novel Approach for Characterizing Quantum Computer Science
职业:量子态复杂性理论:表征量子计算机科学的新方法
- 批准号:
2339116 - 财政年份:2024
- 资助金额:
$ 2.19万 - 项目类别:
Continuing Grant
Low-complexity配列の相分離液滴の分光学的解析法の開発
低复杂度排列相分离液滴光谱分析方法的发展
- 批准号:
23K23857 - 财政年份:2024
- 资助金额:
$ 2.19万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Building Molecular Complexity Through Enzyme-Enabled Synthesis
通过酶合成构建分子复杂性
- 批准号:
DE240100502 - 财政年份:2024
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Early Career Researcher Award
Data Complexity and Uncertainty-Resilient Deep Variational Learning
数据复杂性和不确定性弹性深度变分学习
- 批准号:
DP240102050 - 财政年份:2024
- 资助金额:
$ 2.19万 - 项目类别:
Discovery Projects
Taming the complexity of the law: modelling and visualisation of dynamically interacting legal systems [RENEWAL].
驾驭法律的复杂性:动态交互的法律系统的建模和可视化[RENEWAL]。
- 批准号:
MR/X023028/1 - 财政年份:2024
- 资助金额:
$ 2.19万 - 项目类别:
Fellowship
Career: The Complexity pf Quantum Tasks
职业:量子任务的复杂性
- 批准号:
2339711 - 财政年份:2024
- 资助金额:
$ 2.19万 - 项目类别:
Continuing Grant
22-BBSRC/NSF-BIO Building synthetic regulatory units to understand the complexity of mammalian gene expression
22-BBSRC/NSF-BIO 构建合成调控单元以了解哺乳动物基因表达的复杂性
- 批准号:
BB/Y008898/1 - 财政年份:2024
- 资助金额:
$ 2.19万 - 项目类别:
Research Grant