Elucidation of the Molecular Mechanism of Staphylococcus aureus response to cell wall damage

阐明金黄色葡萄球菌细胞壁损伤反应的分子机制

基本信息

  • 批准号:
    RGPIN-2015-05829
  • 负责人:
  • 金额:
    $ 2.48万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2019
  • 资助国家:
    加拿大
  • 起止时间:
    2019-01-01 至 2020-12-31
  • 项目状态:
    已结题

项目摘要

The proposed research program aims understanding the Staphylococcus aureus response to cell wall damage. Bacterial cell wall is the first line of defense in bacteria and as such its integrity is under constant stress. Cell wall in Gram-positive bacteria consists of a glycopeptide polymer, referred to as peptidoglycan, and a polyanionic polymer covalently attached to peptidoglycan, referred to as wall teichoic acid. Integrity of both polymers is indispensable to bacterial cell growth and division, and in the case of pathogenic bacteria they are indispensable to colonization and host infection.***Genome-based studies on profiling the activity of cell wall inhibitors have revealed that bacteria in general and S. aureus is particular have complex signaling mechanisms in place to sense and respond to cell wall damage. A number of signal transduction and metabolic pathways appear to be involved with the response to cell wall damage, independent of where in the pathway the damage occurs. The details of how the signal-transduction pathways function and work together to respond to cell wall damage are lacking. ***The proposed research program focuses on the molecular mechanisms of signal-transduction by two-component systems VraSR and GraSR and the eukaryotic- like serine/threonine kinase/phosphatase Stk1/Stp of S. aureus. VraSR is considered the sentinel of cell wall integrity. My group was the first to provide the kinetics of signal-transduction by VraSR and revealed the regulatory mechanism of VraR (Belcheva et al. J. Biol. Chem, 2008; Belcheva et al.  Biochemistry 2009). Recent reports show that S. aureus may involve another protein in tuning signaling by VraSR, VraT. GraSR is involved in modulation of wall teichoic acid, and our current research indicates that GraS utilizes a different mechanism than VraSR for signaling (Muzamal et al. F1000 Prime, 2014). Stk1/Stp is involved in regulation of cell wall synthesis and was shown by our group to interact with GraSR (Fridman et al. Biochemistry, 2013). Moreover, we recently discovered that Stk1/Stp phosphorylates a protein involved with cell wall synthesis. ***The objectives of the proposal are two-fold: understand signaling mechanisms of the above systems and their interplay in S. aureus response to cell wall damage. My laboratory has expertise in protein chemistry, molecular biology and microbiology to achieve the above objectives. The proposed research program constitutes a basic research on stress response mechanisms, gene functions and regulation in S. aureus. The knowledge anticipated to be gained in this program will be of great interest to research communities involved in studies of microbial stress responses, cell wall biosynthesis and gene regulation.***********
提出的研究计划旨在了解金黄色葡萄球菌对细胞壁损伤的反应。细菌细胞壁是细菌的第一道防线,因此其完整性受到持续的压力。革兰氏阳性菌细胞壁由糖肽聚合物(称为肽聚糖)和聚阴离子共价附着于肽聚糖的聚合物(称为壁磷壁酸)组成。这两种聚合物的完整性对于细菌细胞的生长和分裂是必不可少的,在致病菌的情况下,它们对于定植和宿主感染是必不可少的。基于基因组的细胞壁抑制剂活性分析研究表明,一般细菌,特别是金黄色葡萄球菌,具有复杂的信号机制来感知和响应细胞壁损伤。许多信号转导和代谢途径似乎与细胞壁损伤的反应有关,而与损伤发生的途径无关。信号转导途径如何发挥作用并共同作用以应对细胞壁损伤的细节尚不清楚。***拟重点研究金黄色葡萄球菌双组分系统VraSR和GraSR以及真核样丝氨酸/苏氨酸激酶/磷酸酶Stk1/Stp信号转导的分子机制。VraSR被认为是细胞壁完整性的哨兵。我的小组首次提供了VraSR信号转导的动力学,并揭示了VraR的调控机制(Belcheva等)。生物。化学,2008;Belcheva等人。生物化学2009)。最近的报道表明,金黄色葡萄球菌可能涉及VraSR信号调节的另一种蛋白VraT。GraSR参与壁壁壁酸的调节,我们目前的研究表明,GraS利用不同于VraSR的信号传导机制(Muzamal等)。F1000 Prime, 2014)。Stk1/Stp参与细胞壁合成的调控,并被我们的团队证明与GraSR相互作用(friedman等)。生物化学,2013)。此外,我们最近发现Stk1/Stp磷酸化了一种参与细胞壁合成的蛋白质。***该提案的目的有两个:了解上述系统的信号机制及其在金黄色葡萄球菌对细胞壁损伤的反应中的相互作用。我的实验室拥有蛋白质化学、分子生物学和微生物学方面的专业知识来实现上述目标。本研究项目是对金黄色葡萄球菌应激反应机制、基因功能及调控的基础性研究。预期在本课程中获得的知识将对参与微生物应激反应,细胞壁生物合成和基因调控研究的研究团体产生极大的兴趣。***********

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

GolemiKotra, Dasantila其他文献

GolemiKotra, Dasantila的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('GolemiKotra, Dasantila', 18)}}的其他基金

Elucidation of the Molecular Mechanism of Staphylococcus aureus Response to Cell-Wall Damage
阐明金黄色葡萄球菌细胞壁损伤反应的分子机制
  • 批准号:
    RGPIN-2020-06105
  • 财政年份:
    2022
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Discovery Grants Program - Individual
Infrastructure to Support Ultra-High Separation of Biological Samples
支持生物样品超高分离度的基础设施
  • 批准号:
    RTI-2022-00531
  • 财政年份:
    2021
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Research Tools and Instruments
Elucidation of the Molecular Mechanism of Staphylococcus aureus Response to Cell-Wall Damage
阐明金黄色葡萄球菌细胞壁损伤反应的分子机制
  • 批准号:
    RGPIN-2020-06105
  • 财政年份:
    2021
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Discovery Grants Program - Individual
Elucidation of the Molecular Mechanism of Staphylococcus aureus Response to Cell-Wall Damage
阐明金黄色葡萄球菌细胞壁损伤反应的分子机制
  • 批准号:
    RGPIN-2020-06105
  • 财政年份:
    2020
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Discovery Grants Program - Individual
Elucidation of the Molecular Mechanism of Staphylococcus aureus response to cell wall damage
阐明金黄色葡萄球菌细胞壁损伤反应的分子机制
  • 批准号:
    RGPIN-2015-05829
  • 财政年份:
    2018
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Discovery Grants Program - Individual
Elucidation of the Molecular Mechanism of Staphylococcus aureus response to cell wall damage
阐明金黄色葡萄球菌细胞壁损伤反应的分子机制
  • 批准号:
    RGPIN-2015-05829
  • 财政年份:
    2017
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Discovery Grants Program - Individual
Elucidation of the Molecular Mechanism of Staphylococcus aureus response to cell wall damage
阐明金黄色葡萄球菌细胞壁损伤反应的分子机制
  • 批准号:
    RGPIN-2015-05829
  • 财政年份:
    2016
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Discovery Grants Program - Individual
Elucidation of the Molecular Mechanism of Staphylococcus aureus response to cell wall damage
阐明金黄色葡萄球菌细胞壁损伤反应的分子机制
  • 批准号:
    RGPIN-2015-05829
  • 财政年份:
    2015
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Discovery Grants Program - Individual
Elucidation of the molecular mechanism of S. aureus response to cell wall damage
阐明金黄色葡萄球菌响应细胞壁损伤的分子机制
  • 批准号:
    312200-2010
  • 财政年份:
    2014
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Discovery Grants Program - Individual
Elucidation of the molecular mechanism of S. aureus response to cell wall damage
阐明金黄色葡萄球菌响应细胞壁损伤的分子机制
  • 批准号:
    312200-2010
  • 财政年份:
    2013
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Discovery Grants Program - Individual

相似国自然基金

Kidney injury molecular(KIM-1)介导肾小管上皮细胞自噬在糖尿病肾病肾间质纤维化中的作用
  • 批准号:
    81300605
  • 批准年份:
    2013
  • 资助金额:
    23.0 万元
  • 项目类别:
    青年科学基金项目
Molecular Plant
  • 批准号:
    31224801
  • 批准年份:
    2012
  • 资助金额:
    20.0 万元
  • 项目类别:
    专项基金项目
Molecular Interaction Reconstruction of Rheumatoid Arthritis Therapies Using Clinical Data
  • 批准号:
    31070748
  • 批准年份:
    2010
  • 资助金额:
    34.0 万元
  • 项目类别:
    面上项目
Molecular Plant
  • 批准号:
    31024802
  • 批准年份:
    2010
  • 资助金额:
    20.0 万元
  • 项目类别:
    专项基金项目
Cellular & Molecular Immunology
  • 批准号:
    30824806
  • 批准年份:
    2008
  • 资助金额:
    20.0 万元
  • 项目类别:
    专项基金项目

相似海外基金

Elucidation of molecular mechanism of age-associated impaired insulin secretion by single cell analysis
通过单细胞分析阐明与年龄相关的胰岛素分泌受损的分子机制
  • 批准号:
    23K18302
  • 财政年份:
    2023
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
Elucidation of the molecular mechanism of transdifferentiation from chondrocytes to osteoblasts by Runx2
Runx2阐明软骨细胞向成骨细胞转分化的分子机制
  • 批准号:
    23K09121
  • 财政年份:
    2023
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of Enhanced Anti-inflammatory Blood Mononuclear Cell Therapy for ARDS and Elucidation of the Molecular Mechanism
ARDS增强抗炎血液单核细胞治疗的进展及分子机制的阐明
  • 批准号:
    23K07659
  • 财政年份:
    2023
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidation of molecular mechanism on lipid biosynthesis, degradation, and its application in oleaginous yeast
脂质生物合成、降解的分子机制阐明及其在产油酵母中的应用
  • 批准号:
    23K19286
  • 财政年份:
    2023
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
Theoretical Elucidation of the Adaptation Mechanism in Molecular Motors and Its Application to Advanced Function Design
分子马达适应机制的理论阐明及其在高级功能设计中的应用
  • 批准号:
    22KJ0505
  • 财政年份:
    2023
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Elucidation of molecular mechanism of betaine-GAT2 connection in the prevention of dementia
阐明甜菜碱-GAT2连接预防痴呆的分子机制
  • 批准号:
    23K06156
  • 财政年份:
    2023
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidation of molecular mechanism of environmental response during the initiation of delignification in white-rot fungi
阐明白腐真菌脱木质素启动过程中环境响应的分子机制
  • 批准号:
    23K19309
  • 财政年份:
    2023
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
Elucidation of the molecular mechanism of recurrent and metastatic cancer using a breast cancer mouse model and establishment of a preventive method
利用乳腺癌小鼠模型阐明复发和转移性癌症的分子机制并建立预防方法
  • 批准号:
    23K18237
  • 财政年份:
    2023
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
Elucidation of the molecular mechanism controlling cannibalistic behavior in Drosophila
阐明控制果蝇同类相食行为的分子机制
  • 批准号:
    23KJ1649
  • 财政年份:
    2023
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Elucidation of molecular mechanism of the homeostasis between brain and peripheral organs by sleep
阐明睡眠影响大脑与周围器官稳态的分子机制
  • 批准号:
    23KJ0266
  • 财政年份:
    2023
  • 资助金额:
    $ 2.48万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了