Understanding VAV2-dependent cell migration process through characterization of interactions with ERM proteins.

通过表征与 ERM 蛋白的相互作用来了解 VAV2 依赖性细胞迁移过程。

• 批准号: RGPIN-2019-04082
• 负责人: Marignani, Paola
• 金额: $ 3.06万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=688568
• 关键词: Understanding; VAV2; dependent; cell; migration

The reorganization of cytoskeleton is a highly conserved molecular process that is required by single and multi-cell organisms to survive. The cytoskeleton is a complex structure comprised of hundreds of molecules that work synchronously to maintain the infrastructure of the cell or to move cells in response to stimuli. *** Our overarching goal is to understand molecular processes that regulate cell migration. To this end, our group is working on deciphering how a new protein-protein interaction we discovered between the guanine nucleotide exchange factor VAV2 and ERM-family member EZRIN, work together to regulate cell migration. Our work in progress shows that these two proteins interact in vivo and contribute to signalling cascades that regulate cell migration in response to stimuli. The outcomes from our program will expand our knowledge of the molecular processes that are involved in mediating the signalling events that are necessary for biological processes that include cytoskeleton reorganization, cell migration, and phagocytosis. We are privileged to have made the discovery of a new protein-protein interaction and to be able to continue our program on a long-term basis. Undergraduate and graduate students contributing to this program will experience diverse training in molecular and cellular biology, informatics analysis and knowledge translation.

细胞骨架的重组是一个高度保守的分子过程，是单细胞和多细胞生物生存所必需的。细胞骨架是由数百个分子组成的复杂结构，这些分子同步工作以维持细胞的基础结构或响应刺激而移动细胞。我们的首要目标是了解调节细胞迁移的分子过程。为此，我们的团队正在努力破译我们在鸟嘌呤核苷酸交换因子VAV2和erm家族成员EZRIN之间发现的一种新的蛋白质-蛋白质相互作用如何共同调节细胞迁移。我们正在进行的工作表明，这两种蛋白在体内相互作用，并有助于信号级联反应，调节细胞对刺激的迁移。我们项目的成果将扩展我们对介导信号事件的分子过程的知识，这些信号事件是包括细胞骨架重组、细胞迁移和吞噬在内的生物过程所必需的。我们很荣幸发现了一种新的蛋白质与蛋白质之间的相互作用，并能够长期继续我们的项目。参与本课程的本科生和研究生将在分子和细胞生物学、信息学分析和知识翻译方面接受多样化的培训。