Developing robust canola lines with enhanced shatter tolerance and improved seed degreening traits

开发具有增强抗碎性和改善种子度性状的健壮双低油菜系

• 批准号: 522392-2017
• 负责人: Samuel, Marcus
• 金额: $ 5.83万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=692125
• 关键词: Developing; robust; canola; lines; enhanced

Canola is the major cash crop in Canada which contributes ~ 26.7 billion dollars annually to the Canadian economy. Canadian canola, particularly in Western Canada is consistently exposed to unfavorable weather conditions such as drought and untimely frost that results in increased early shattering of pods and increased green seed content in canola seeds. We have recently identified the molecular pathway involving the transcription factor, Abscisic acid insensitive 3 (ABI3), that is responsible for protecting canola against frost-induced green seeds. We now have evidence that overexpression of this pathway in canola can improve shatter tolerance and confer degreening of canola seeds even after frost. Through this proposal, in partnership with NUTRIEN, we aim to achieve improved shatter tolerance and enhanced degreening in canola through exploiting multiple approaches including a non-GM approach. In the short-term, during the course of this project, we propose to deliver modified canola germplasm that are both shatter tolerant and green seed resistant to Nutrien, which will be immediately used in their double haploid production system to deliver high quality canola lines for use in Canada. From an academic and scientific perspective, this proposal will allow us generate new knowledge on hormonal cross-talk which can mediate these agronomic traits, which has not been explored thus far. From a technological standpoint, this proposal will also allow us to establish the CRISPR-Cas9 platform in canola which will help us with a number of future research projects including collaborative projects with Nutrien. Through this project, we will generate canola germplasms that will have immediate benefit to farmers by generating increased yield due to enhanced shatter tolerance, and also by producing higher grade canola with less green seed count.

油菜是加拿大的主要经济作物，每年为加拿大经济贡献约267亿美元。加拿大油菜籽，特别是加拿大西部的油菜籽持续暴露于不利的天气条件，如干旱和不合时宜的霜冻，这导致豆荚的早期破碎增加和油菜籽中绿色种子含量增加。我们最近已经确定了分子途径，涉及转录因子，脱落酸不敏感3（ABI 3），这是负责保护油菜对霜冻诱导的绿色种子。我们现在有证据表明，在油菜中过表达这一途径可以提高油菜种子的抗裂性，甚至在霜冻后也能使油菜种子退化。通过这一提议，我们与NUTRIEN合作，旨在通过利用多种方法（包括非转基因方法）来提高油菜籽的耐碎性和提高其去劣性。在短期内，在该项目的过程中，我们建议提供改良的油菜种质，这些种质既耐破碎又耐Nutrien的绿色种子，这些种质将立即用于他们的双单倍体生产系统，以提供高质量的油菜品系供加拿大使用。从学术和科学的角度来看，这一建议将使我们能够产生关于激素串扰的新知识，这些激素串扰可以介导这些农艺性状，迄今为止尚未探索过。从技术的角度来看，这一提议还将使我们能够在油菜籽中建立CRISPR-Cas9平台，这将有助于我们开展一些未来的研究项目，包括与Nutrien的合作项目。通过这个项目，我们将产生油菜胚芽，通过提高耐破碎性提高产量，同时生产出绿色种子较少的高品质油菜，从而为农民带来直接利益。