Developing robust canola lines with enhanced shatter tolerance and improved seed degreening traits

开发具有增强抗碎性和改善种子度性状的健壮双低油菜系

• 批准号: 522392-2017
• 负责人: Samuel, Marcus
• 金额: $ 5.83万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=697639
• 关键词: Developing; robust; canola; lines; enhanced

Canola is the major cash crop in Canada which contributes ~ 26.7 billion dollars annually to the Canadian economy. Canadian canola, particularly in Western Canada is consistently exposed to unfavorable weather conditions such as drought and untimely frost that results in increased early shattering of pods and increased green seed content in canola seeds. We have recently identified the molecular pathway involving the transcription factor, Abscisic acid insensitive 3 (ABI3), that is responsible for protecting canola against frost-induced green seeds. We now have evidence that overexpression of this pathway in canola can improve shatter tolerance and confer degreening of canola seeds even after frost. Through this proposal, in partnership with NUTRIEN, we aim to achieve improved shatter tolerance and enhanced degreening in canola through exploiting multiple approaches including a non-GM approach. In the short-term, during the course of this project, we propose to deliver modified canola germplasm that are both shatter tolerant and green seed resistant to Nutrien, which will be immediately used in their double haploid production system to deliver high quality canola lines for use in Canada. From an academic and scientific perspective, this proposal will allow us generate new knowledge on hormonal cross-talk which can mediate these agronomic traits, which has not been explored thus far. From a technological standpoint, this proposal will also allow us to establish the CRISPR-Cas9 platform in canola which will help us with a number of future research projects including collaborative projects with Nutrien. Through this project, we will generate canola germplasms that will have immediate benefit to farmers by generating increased yield due to enhanced shatter tolerance, and also by producing higher grade canola with less green seed count.

低芥酸菜籽是加拿大的主要经济作物，每年为加拿大经济贡献约267亿美元。加拿大的低芥酸菜籽（尤其是在加拿大西部）始终暴露于不利的天气状况，例如干旱和不合时宜的霜冻，从而增加了豆荚的早期破碎，并增加了低芥酸菜子种子中的绿色种子含量。我们最近确定了涉及转录因子脱离酸不敏感3（ABI3）的分子途径，该途径负责保护低芥酸菜籽体免受霜冻诱导的绿色种子的影响。现在，我们有证据表明，即使在霜冻后，菜籽中这种途径的过表达也可以提高菜籽种子的破碎耐受性并赋予脱胶。通过这项建议，与Nutrien合作，我们旨在通过利用包括非GM方法在内的多种方法来提高低芥酸菜籽的破碎耐受性并增强脱土。在短期内，在该项目的过程中，我们建议提供改良的低铁质种质，这些种质既耐受耐受性，又具有绿色种子对Nutrien的耐药性，该种子将立即用于其双倍单倍体生产系统中，以提供高质量的低芥酸菜籽线，以便在加拿大使用。从学术和科学的角度来看，该提案将使我们对荷尔蒙交叉言论产生新的知识，这些知识可以调解这些农艺特征，到目前为止尚未探索。从技术的角度来看，该提案还将使我们能够在低芥酸菜籽中建立CRISPR-CAS9平台，这将帮助我们进行许多未来的研究项目，包括与Nutrien的合作项目。通过这个项目，我们将产生低油菜籽种系，通过增强的破碎耐受性，以及生产更高级别的低芥酸菜籽油，并以较少的绿色种子数量产生更高的低菜籽，从而产生了立即利益，从而为农民带来立即的好处。