Oroboros FluoRespirometer for the real time measurement of bioenergetics

申请人在与代谢、营养、毒理学和疾病结局相关的广泛学科的出版物方面有着良好的记录。申请人的主要重点是获得测量线粒体生物能量学所必需的工具，这是代谢研究中的黄金标准，因为线粒体在氧气存在下燃烧营养素以产生驱动大多数生理过程的通用能量货币ATP。我们的研究团队正在提交申请，以获得Oroboros O2 K-FluoRespirometer与Motic Microscrope和PBI组织切碎机，以测量麦吉尔大学人类营养学院的生物能量学。O2 K配备了两个密封室，其中装有O2传感器和一个荧光计。这种配置允许线粒体生物能量学的容易和高度准确的测量。可用性将使我们的研究团队每天都能访问对我们的研究至关重要的资产。分别需要显微镜和粉碎机来制备用于分析的样品。O2 K是通用的，易于使用，并带有它的操作和维护成本低，使其成为理想的HQP培训和进行NSERC资助的研究。O2 K或同等产品目前在麦克唐纳校园不存在。Ryan Mailloux博士（主要申请人）的NSERC发现补助金被用于资助两个主要的研究重点; 1）了解线粒体如何产生活性氧（ROS）用于细胞信号传导，2）氧化还原信号在控制线粒体响应环境线索中的功能。他的工作对细胞如何利用线粒体ROS和氧化还原信号改变细胞行为以响应营养干预产生了重要的见解。他令人兴奋的发现还确定了治疗/预防肥胖和相关疾病的新的潜在目标。他将使用O2 K继续绘制这些途径，并以确定治疗代谢性疾病的新靶点为长期愿景。 * Kubow博士（共同申请人）的研究包括研究营养干预和毒理学暴露对肠道/肝脏炎症的影响，特别是由肠道微生物组和消化后营养素和毒素的微生物代谢产物介导的影响。他携带了许多NSERC赠款，包括一个加速赠款，这是用来构建他独特的人类消化道模型。他打算与Mailloux博士合作使用O2 K系统，以进一步支持他的NSERC资助的研究。预计在这种能力中使用O2 K将显着推进我们对肠道-微生物组-肝脏轴如何影响线粒体生物能量学以响应营养干预和毒素的理解。最后，O2 K将通过为线粒体生物能量学的询问提供最先进的工具来增强两个申请人的基础和应用研究活动。********

The applicants have a strong track record regarding publications in a wide array of disciplines pertaining to metabolism, nutrition, toxicology, and disease outcomes. The major focus of the applicants is to obtain the tools necessary to measure mitochondrial bioenergetics, a gold-standard in metabolic research because mitochondria combust nutrients in the presence of oxygen to make the universal energy currency ATP, which drives most physiological processes. Our research team is submitting a request to obtain an Oroboros O2K-FluoRespirometer with Motic Microscrope and PBI Tissue Shredder to measure bioenergetics in the School of Human Nutrition at McGill. The O2K is equipped with two air-tight chambers housing O2 sensors and a fluorometer. This configuration allows for the easy and highly accurate measurement of mitochondrial bioenergetics. The availability will provide our research team daily access to an asset that is critical for our research. The Microscope and Shredder, respectively, are required to prepare samples for analysis. The O2K is versatile, easy to use, and carries with it a low operation and maintenance cost, making it ideal for HQP training and conducting NSERC-funded research. The O2K or equivalent currently does not exist at the Macdonald campus.***Dr. Ryan Mailloux's (principle applicant) NSERC Discovery Grant is being used to fund two major research foci; 1) understanding how mitochondria generate reactive oxygen species (ROS) for cell signaling, 2) the function of redox signals in controlling mitochondria in response to environmental cues. His work has led to important insights into how cells use mitochondrial ROS and redox signals to change cell behavior in response to nutritional interventions. His exciting findings have also identified new potential targets to treat/prevent obesity and related disorders. He will use the O2K to continue mapping out these pathways with the long-term vision of identifying novel targets for the treatment of metabolic diseases. ***Dr. Kubow's (co-applicant) research includes study of the impact of nutritional interventions and toxicological exposures on intestinal/hepatic inflammation, particularly as mediated by the gut microbiome and post-digestive microbial metabolites of nutrients and toxins. He carries numerous NSERC Grants, including an Accelerate Grant which was used to construct his unique model of the human digestive tract. He intends to use the O2K system in collaboration with Dr. Mailloux to further support his NSERC-funded research. It is anticipated that using the O2K in this capacity will significantly advance our understanding of how the gut-microbiome-liver axis influences mitochondrial bioenergetics in response to nutritional interventions and toxins.***In closing, the O2K will augment the fundamental and applied research activities of the two applicants by providing state-of-the-art tools for the interrogation of mitochondrial bioenergetics. ********