Oroboros FluoRespirometer for the real time measurement of bioenergetics

申请人在代谢、营养、毒理学和疾病结果等广泛学科的出版物方面有良好的记录。申请人的主要重点是获得测量线粒体生物能量学所需的工具，这是代谢研究的黄金标准，因为线粒体在氧气存在的情况下燃烧营养物质，产生通用的能量货币ATP，这驱动了大多数生理过程。我们的研究团队正在提交一份申请，要求获得一台Oroboros o2k -荧光肺气计，带有Motic显微镜和PBI组织碎纸机，用于测量麦吉尔人类营养学院的生物能量学。O2K配备了两个气密室，容纳氧气传感器和一个荧光计。这种配置允许线粒体生物能量学的简单和高度精确的测量。可用性将为我们的研究团队提供每日访问对我们的研究至关重要的资产。分别需要显微镜和碎纸机来准备样品进行分析。O2K是多功能的，易于使用，并且具有较低的操作和维护成本，使其成为HQP培训和进行nserc资助研究的理想选择。麦克唐纳校区目前不存在O2K或同等学历。Ryan Mailloux（主要申请人）的NSERC发现基金被用于资助两个主要的研究重点；1)了解线粒体如何产生活性氧（ROS）用于细胞信号传导，2)氧化还原信号在控制线粒体响应环境信号中的作用。他的工作对细胞如何利用线粒体ROS和氧化还原信号来改变细胞行为以响应营养干预产生了重要的见解。他令人兴奋的发现也确定了治疗/预防肥胖及相关疾病的新潜在目标。他将利用O2K继续绘制这些途径，并以确定治疗代谢性疾病的新靶点为长期目标。* * *博士。Kubow（共同申请人）的研究包括营养干预和毒理学暴露对肠道/肝脏炎症的影响，特别是由肠道微生物群和消化后微生物代谢产物介导的营养和毒素。他获得了许多国家科学研究委员会的资助，包括一项加速资助，用于构建他独特的人类消化道模型。他打算与Mailloux博士合作使用O2K系统来进一步支持他的nserc资助的研究。预计在这种情况下使用O2K将大大提高我们对肠道-微生物组-肝脏轴在营养干预和毒素反应中如何影响线粒体生物能量学的理解。***最后，O2K将通过提供最先进的工具来审问线粒体生物能量学，从而增加两位申请人的基础和应用研究活动。* * * * * * * *

The applicants have a strong track record regarding publications in a wide array of disciplines pertaining to metabolism, nutrition, toxicology, and disease outcomes. The major focus of the applicants is to obtain the tools necessary to measure mitochondrial bioenergetics, a gold-standard in metabolic research because mitochondria combust nutrients in the presence of oxygen to make the universal energy currency ATP, which drives most physiological processes. Our research team is submitting a request to obtain an Oroboros O2K-FluoRespirometer with Motic Microscrope and PBI Tissue Shredder to measure bioenergetics in the School of Human Nutrition at McGill. The O2K is equipped with two air-tight chambers housing O2 sensors and a fluorometer. This configuration allows for the easy and highly accurate measurement of mitochondrial bioenergetics. The availability will provide our research team daily access to an asset that is critical for our research. The Microscope and Shredder, respectively, are required to prepare samples for analysis. The O2K is versatile, easy to use, and carries with it a low operation and maintenance cost, making it ideal for HQP training and conducting NSERC-funded research. The O2K or equivalent currently does not exist at the Macdonald campus.***Dr. Ryan Mailloux's (principle applicant) NSERC Discovery Grant is being used to fund two major research foci; 1) understanding how mitochondria generate reactive oxygen species (ROS) for cell signaling, 2) the function of redox signals in controlling mitochondria in response to environmental cues. His work has led to important insights into how cells use mitochondrial ROS and redox signals to change cell behavior in response to nutritional interventions. His exciting findings have also identified new potential targets to treat/prevent obesity and related disorders. He will use the O2K to continue mapping out these pathways with the long-term vision of identifying novel targets for the treatment of metabolic diseases. ***Dr. Kubow's (co-applicant) research includes study of the impact of nutritional interventions and toxicological exposures on intestinal/hepatic inflammation, particularly as mediated by the gut microbiome and post-digestive microbial metabolites of nutrients and toxins. He carries numerous NSERC Grants, including an Accelerate Grant which was used to construct his unique model of the human digestive tract. He intends to use the O2K system in collaboration with Dr. Mailloux to further support his NSERC-funded research. It is anticipated that using the O2K in this capacity will significantly advance our understanding of how the gut-microbiome-liver axis influences mitochondrial bioenergetics in response to nutritional interventions and toxins.***In closing, the O2K will augment the fundamental and applied research activities of the two applicants by providing state-of-the-art tools for the interrogation of mitochondrial bioenergetics. ********