Oroboros FluoRespirometer for the real time measurement of bioenergetics

申请者在与新陈代谢、营养、毒理学和疾病结局相关的广泛学科的出版物方面有着良好的记录。申请者的主要关注点是获得测量线粒体生物能量学所需的工具，这是新陈代谢研究中的黄金标准，因为线粒体在氧气存在的情况下燃烧营养物质，使通用能源货币三磷酸腺苷(ATP)驱动大多数生理过程。我们的研究团队正在提交一份申请，要求获得一台Oroboros O2K-荧光呼吸仪，该仪器带有运动微管和PBI组织碎纸机，用于在麦吉尔人类营养学院测量生物能量学。O2K配备了两个密封室，里面装有氧气传感器和一个荧光计。这种配置允许轻松和高精度地测量线粒体生物能量学。这一可用性将使我们的研究团队每天都能访问对我们的研究至关重要的资产。显微镜和碎纸机分别需要准备用于分析的样品。O2K功能多样，使用方便，运行和维护成本低，是HQP培训和进行NSERC资助研究的理想之选。Macdonald园区目前还不存在O2K或类似物。*Ryan Mailloux博士(主要申请者)的NSERC Discovery Grant将用于资助两个主要研究重点：1)了解线粒体如何产生细胞信号的活性氧物种(ROS)；2)氧化还原信号在控制线粒体响应环境提示方面的功能。他的工作导致了对细胞如何利用线粒体ROS和氧化还原信号来改变细胞行为以响应营养干预的重要见解。他令人兴奋的发现还确定了治疗/预防肥胖症和相关疾病的新的潜在目标。他将使用O2K继续绘制这些路径，并着眼于确定治疗代谢性疾病的新靶点。*库博博士(共同申请者)的研究包括研究营养干预和毒物暴露对肠道/肝脏炎症的影响，特别是通过肠道微生物群和消化后营养物质和毒素的微生物代谢物的影响。他拥有许多NSERC奖助金，包括一个用于构建他独特的人类消化道模型的Accelerate Grant。他打算与梅鲁博士合作使用O2K系统，以进一步支持他由NSERC资助的研究。预计在这种能力下使用O2K将大大促进我们对肠道-微生物-肝脏轴如何影响线粒体生物能量学的理解，以响应营养干预和毒素。*最后，O2K将通过提供询问线粒体生物能量学的最先进工具，加强两个申请者的基础和应用研究活动。********

The applicants have a strong track record regarding publications in a wide array of disciplines pertaining to metabolism, nutrition, toxicology, and disease outcomes. The major focus of the applicants is to obtain the tools necessary to measure mitochondrial bioenergetics, a gold-standard in metabolic research because mitochondria combust nutrients in the presence of oxygen to make the universal energy currency ATP, which drives most physiological processes. Our research team is submitting a request to obtain an Oroboros O2K-FluoRespirometer with Motic Microscrope and PBI Tissue Shredder to measure bioenergetics in the School of Human Nutrition at McGill. The O2K is equipped with two air-tight chambers housing O2 sensors and a fluorometer. This configuration allows for the easy and highly accurate measurement of mitochondrial bioenergetics. The availability will provide our research team daily access to an asset that is critical for our research. The Microscope and Shredder, respectively, are required to prepare samples for analysis. The O2K is versatile, easy to use, and carries with it a low operation and maintenance cost, making it ideal for HQP training and conducting NSERC-funded research. The O2K or equivalent currently does not exist at the Macdonald campus.***Dr. Ryan Mailloux's (principle applicant) NSERC Discovery Grant is being used to fund two major research foci; 1) understanding how mitochondria generate reactive oxygen species (ROS) for cell signaling, 2) the function of redox signals in controlling mitochondria in response to environmental cues. His work has led to important insights into how cells use mitochondrial ROS and redox signals to change cell behavior in response to nutritional interventions. His exciting findings have also identified new potential targets to treat/prevent obesity and related disorders. He will use the O2K to continue mapping out these pathways with the long-term vision of identifying novel targets for the treatment of metabolic diseases. ***Dr. Kubow's (co-applicant) research includes study of the impact of nutritional interventions and toxicological exposures on intestinal/hepatic inflammation, particularly as mediated by the gut microbiome and post-digestive microbial metabolites of nutrients and toxins. He carries numerous NSERC Grants, including an Accelerate Grant which was used to construct his unique model of the human digestive tract. He intends to use the O2K system in collaboration with Dr. Mailloux to further support his NSERC-funded research. It is anticipated that using the O2K in this capacity will significantly advance our understanding of how the gut-microbiome-liver axis influences mitochondrial bioenergetics in response to nutritional interventions and toxins.***In closing, the O2K will augment the fundamental and applied research activities of the two applicants by providing state-of-the-art tools for the interrogation of mitochondrial bioenergetics. ********