Back to Capacity: Replacement of a 20-year Old Non-servicable Automated Gas Chromatograph for the Determination of Greenhouse Gas Emissions from Agriculture

恢复产能：更换已使用 20 年、无法维修的自动气相色谱仪，用于测定农业温室气体排放

• 批准号: RTI-2020-00451
• 负责人: Tenuta, Mario
• 金额: $ 7.59万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=692869
• 关键词: Back; Capacity; Replacement; 20; year

Dr. Tenuta's Applied Soil Ecology Laboratory has fully utilized two automated gas chromatographs since 2005 for the determination of the emissions of the greenhouse gases nitrous oxide, methane, and carbon dioxide from agricultural soils and manure. Recently, the oldest of the instruments purchased in 1999 became inoperable and past its serviceable lifespan. Dr. Tenuta's lab urgently requires replacement of the system to analyze a backlog of samples collected this year and to start new funded projects dependent on the collection of even a greater number of samples next year. Other instrumentation is not available at the University, and commercial services are unavailable. The proposed instrument is similar in configuration to the one it replaces as the needs of gas analysis and sample handling have not changed. The instrument is configured for high throughput with an autosampler and capable of analysis of 250 samples per day of air-tight sample vials. The three most important greenhouse gases for agriculture are analyzed simultaneously as the instrument is equipped with detectors for each gas. The lab strives to Better Soil Nitrogen Use Towards Improved Crop and Environmental Health. A keystone of the research is the comparison of farmer management practices that reduce greenhouse gas emissions. The primary method employed to determine emissions is the collection of gases accumulated within chambers on the soil or manure pile surfaces. The gas chromatograph is ideally suited to high throughput analysis of the gases stored in glass vials. The instrument is also central to the training of students and other highly qualified personnel in the operation, analysis and quality control of gas concentration results. The operation and results from the instrument bring full circle the training of HQP from hypothesis generation, setup, and maintenance of field study sites and treatments, gas collection, gas chromatograph operation and analysis, processing of gas results to emission rates, statistical analyses, and conclusion to hypotheses and communication of findings. A per sample analysis fee for each of the lab's samples and those of his collaborators supports the operational readiness of the instrument as well as training in the use of the instrument and its results. An anticipated eight graduate and nine undergraduate students and two post-doctoral fellows will benefit from the instrument being central to their training in Dr. Tenuta's lab. He is highly committed to fostering the removal of barriers facing women and minorities in the soil sciences. This includes providing training in the use of the instrument regardless of a student's gender, race, and beliefs. Training on the instrument is tailored to the individual's confidence and experience in the previous operation of scientific equipment bringing all to a level of familiarity and sureness to benefit their emerging careers.

自2005年以来，Tenuta博士的应用土壤生态实验室充分利用了两台自动气相色谱仪来测定农田土壤和粪便中的温室气体一氧化二氮、甲烷和二氧化碳的排放量。最近，1999年购买的最旧的仪器变得无法操作，超过了其使用寿命。Tenuta博士的实验室迫切需要更换系统，以分析今年收集的积压样本，并启动新的资助项目，这取决于明年收集的样本数量将会更多。该大学没有其他仪器，也没有商业服务。由于气体分析和样品处理的需要没有改变，建议的仪器在结构上与它所取代的仪器相似。该仪器配置为具有自动取样器的高通量，每天能够分析250个密封样品瓶的样品。同时分析了对农业最重要的三种温室气体，因为仪器配备了每种气体的探测器。该实验室致力于更好地利用土壤氮素，以改善作物和环境健康。这项研究的一个重点是比较农民减少温室气体排放的管理做法。用来确定排放量的主要方法是收集土壤或粪便堆表面的室内积聚的气体。该气相色谱仪非常适合对储存在玻璃瓶中的气体进行高通量分析。该仪器也是培训学生和其他高素质人员操作、分析和控制气体浓缩结果的核心。仪器的运行和结果使HQP的培训从假设的产生、现场研究地点和处理的建立和维护、气体采集、气相色谱仪操作和分析、气体结果的处理到排放率、统计分析、结论到假设和结果的交流，形成了完整的循环。实验室和他的合作者的每个样本的每个样本的分析费支持仪器的操作准备情况以及关于使用仪器及其结果的培训。预计将有8名毕业生、9名本科生和2名博士后研究员受益于该仪器，该仪器是他们在Tenuta博士实验室进行培训的核心。他高度致力于推动消除妇女和少数群体在土壤科学方面面临的障碍。这包括提供使用乐器的培训，而不考虑学生的性别、种族和信仰。该仪器的培训是根据个人对以前操作科学设备的信心和经验而量身定做的，使所有人都达到熟悉和确信的程度，从而有利于他们新出现的职业生涯。