Upgrades to an advanced multiphoton microscope with unique equipment to improve tissue access for intravital imaging and improved image stability

升级到先进的多光子显微镜，配备独特的设备，可改善活体成像的组织通道并提高图像稳定性

• 批准号: RTI-2020-00540
• 负责人: Kerfoot, Steven
• 金额: $ 7.72万
• 依托单位: University of Western Ontario
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=692904
• 关键词: Upgrades; advanced; multiphoton; microscope; unique

Intravital (live tissue) imaging is a powerful tool to directly visualize cells and their behaviour within their appropriate anatomical context. This technology has many applications to different fields of cell biology, immunology, neuroscience, and other investigations of cell physiology. This application is to support the acquisition of two upgrades to an existing multiphoton confocal microscope that will overcome some of the challenges inherent to imaging live tissues. Tissue movement due to physiological processes, such as breathing, remains a technical hurdle to intravital microscopy. To address this, we propose to acquire an Image Stabilization Tripod that physically moves the microscope objective to match the biological movement of the tissue. Another challenge to intravital microscopy is access to the tissue itself. Standard multiphoton microscope objectives are large compared to typical small animal models (ex. mice and rats), and sometimes extensive surgery is required in order to make sufficient space. To address this issue, we propose to acquire a Needle Objective Endoscope, that uses a very thin (<1mm), rotating probe as a periscope. This will allow access to deep or otherwise inaccessible tissues with minimal surgery. Together, these upgrades will considerably enhance the capabilities of the existing microscope, increase the efficiency and types of studies that can be performed using it, and broaden the user base and the number of trainees that have access to this important imaging technology.

活体（活组织）成像是一种强大的工具，可以直接可视化细胞及其在适当解剖环境中的行为。这项技术在细胞生物学、免疫学、神经科学和其他细胞生理学研究的不同领域有许多应用。该应用程序是支持收购两个升级到现有的多光子共聚焦显微镜，将克服一些固有的挑战成像活组织。由于生理过程（如呼吸）引起的组织运动仍然是活体显微镜的技术障碍。为了解决这个问题，我们建议获得一个图像稳定三脚架，物理移动显微镜物镜，以匹配组织的生物运动。活体显微镜的另一个挑战是进入组织本身。标准的多光子显微镜物镜与典型的小动物模型（例如，小鼠和大鼠），有时需要进行大范围手术以获得足够的空间。为了解决这个问题，我们建议获得一种针式物镜内窥镜，它使用非常薄（<1 mm）的旋转探头作为潜望镜。这将允许以最小的手术进入深部或以其他方式难以进入的组织。总之，这些升级将大大提高现有显微镜的能力，提高效率和类型的研究，可以使用它进行，并扩大用户群和人数的受训者，有机会获得这一重要的成像技术。