A novel bio-pesticide to control bacterial diseases on food crops-Phase 2

细菌性植物病原体会降低作物产量，从而导致种植者的收入损失。控制粮食作物细菌感染的产品市场很大，全球每年的支出为3亿至5亿美元。现有的解决方案是铜基农药和二硫代氨基甲酸盐（如代森锰锌）的混合物。不幸的是，这些混合物是不够的，因为它们的功效由于细菌病原体产生的耐药性而下降。此外，它们可以通过给植物施加压力，使它们从一开始就减产，从而降低作物产量。最后，它们对土壤和水造成严重的环境危害。该项目的重点是利用生物技术开发创新、具有市场竞争力和环境可持续性的作物保护产品，这些产品在控制粮食作物上的植物致病性黄单胞菌方面比市场标准更有效。生物农药是农业行业中增长最快的部分之一，其驱动因素有以下几个：(a)消费者要求食品不含有毒农药；(b)政府正在响应其公民的需求，并推动生物农药的开发和注册，作为一种替代品；(c)生产者正在积极寻找现有传统产品的替代品，因为存在耐药性问题。这个建议是一个正在进行的项目的延续。提出了几个里程碑，旨在最终开发基于生物活性代谢物的产品，这些代谢物是由先前分离的细菌产生的，证明可以抑制一系列植物致病性黄单胞菌。将进行实地研究，以在粮食作物上测试新产品。最后，我们将开始开发一种基于发酵的制造工艺，并运行中试批次，以使新产品为商业化做好准备。最终产品将是满足加拿大市场迫切需求的生物农药产品。

Bacterial phytopathogens decrease crop yields, which translates to lost revenue for growers. The market for products that control bacterial infections on food crops is large, with annual spend of $300-500M worldwide. The incumbent solution is a mixture of copper-based pesticides and dithiocarbamates like mancozeb. Unfortunately, these mixtures are inadequate because they have falling efficacy due to resistance built up by bacterial pathogens. Furthermore, they can reduce crop yields by stressing plants, causing them to produce less from the outset. Finally, they cause significant environmental harm to soil and water.The focus of this project is to use biotechnology to develop innovative, market competitive, and environmentally sustainable crop protection products that are more efficacious than market standards in controlling phytopathogenic Xanthomonas bacteria on food crops. Bio-pesticides are one of the fastest growing segments in the agricultural industry, driven by a number of factors: (a) consumers are demanding food that is free of toxic pesticides, (b) governments are responding to the demands of its citizens, and pushing for the development and registration of bio-pesticides as an alternative, (c) producers are actively seeking substitutes for existing, conventional products given resistance issues.This proposal is the continuation of an ongoing project. Several milestones are proposed that will aim at finalizing the development of product based bioactive metabolites produced by previously isolated bacteria demonstrated to inhibit a range of phytopathogenic Xanthomonas species. Field studies will be performed to test the new products on food crops. Finally, we will initiate the development of a manufacturing process based on fermentation and run pilot batches to get the new products ready for commercialization. The final product will be a bio-pesticide products that fill pressing needs in the Canadian market.