A Seahorse analyzer for the multiparametric study of cellular metabolism in prokaryotes and eukaryotes.

研究范围：该仪器将用于原核和真核细胞代谢的前沿基础科学研究。研究项目范围从细菌抗生素耐药性的分子分析到控制代谢变化的许多途径的复杂表征。这些变化发生在对环境压力的反应或在细胞发育和分化过程中。该仪器支持的项目可分为四个主要主题：免疫代谢、神经系统的发育/修复、宿主-病原体相互作用和药物发现。******研究团队：仪器由9名申请者的研究人员共享。所有这些实验室都位于蒙特里萨大学的主校区，是医学院（微生物学-感染学-免疫学、病理学-细胞生物学和药理学-生理学）和药学院的一部分。到目前为止，已经确定了计划使用该仪器的另外七名同事。他们中的大多数已经获得了NSERC的资助。******仪器：我们希望获得一台最先进的XFe96海马细胞外通量分析仪，能够在各种类型的细胞中实时同时分析细胞外酸化率（ECAR）和耗氧量（OCR）。尽管其技术复杂，该分析仪可以由所有的研究人员经过简短的实践培训操作。该分析仪将位于中心，是微生物学、传染病学和免疫学部门核心设施的一部分。******仪器、操作、HQP培训和可持续性的需要：海马提供的代谢读数提供了细胞生理学和完整性的关键信息。目前迫切需要该分析仪来提高细胞和全身代谢分析研究项目的深度和影响。不幸的是，校园里没有这样的分析仪，这使得我们的研究人员在获得资助和在高影响力期刊上发表他们的发现时缺乏竞争力。重要的是，所要求的仪器代表了我们核心设施急需的技术补充。新工具的可持续性是由收取用户费用保证的。***我们估计，仅在申请人的实验室中，在未来3年内将有30多名学员使用该分析仪。重要的是，Seahorse分析仪是一种仪器，用户可以围绕它进行交互，共享知识、协议和试剂，催化网络并促进新的合作。细胞基本功能的多参数代谢评估对于实现我们提出的目标至关重要。从长远来看，这些工具将导致更好的培训和更全面的研究。我们的发现可能会发展成为感染、自身免疫、神经系统疾病和癌症的创新治疗方法，这些都具有重要的社会经济影响

Scope of investigations: The instrument will be used to conduct cutting edge basic science research on prokaryotic and eukaryotic cell metabolism. The research projects range from the molecular analysis of bacterial antibiotic resistance to the complex characterization of the many pathways controlling metabolic changes. These changes occur in response to environmental stress or during cell development and differentiation. The projects to be supported by this instrument can be grouped under four major themes: Immunometabolism, development/repair in the nervous system, host-pathogens interactions, and drug discovery.******The research team: The instrument will be shared by the research personnel of the 9 applicants. All these labs are located on the main campus of Université de Montréal and are part of the Faculty of Medicine (departments of Microbiology-Infectiology-Immunology, Pathology-Cell Biology and Pharmacology-Physiology) and the Faculty of Pharmacy. Seven additional colleagues who plan to use the instrument have been identified so far. Most of them already hold NSERC grants.******The instrument: We wish to acquire a state-of-the-art XFe96 Seahorse extracellular flux analyzer capable of performing the simultaneous analysis of extracellular acidification rate (ECAR) and oxygen consumption (OCR) in various types of cells and in real time. Despite its technological complexity, this analyzer can be operated by all research personnel following a short hands-on training. The analyzer will be centrally located and part of the core facility of the Department of Microbiology, Infectiology and immunology.******Need of the instrument, operations, training of HQP and sustainability: The metabolic readouts provided by the Seahorse yield key information on cellular physiology and integrity. The analyzer is urgently required to boost the depth and impact of the current research project focused on cellular and whole body metabolic analysis. Unfortunately, no such analyzer is available on the campus, making our researchers less competitive for grants and for the publication of their findings in high impact journals. Importantly, the requested instrument represents a much needed technological addition to our core facility. Sustainability of the new instrument is guaranteed by the collection of user fees.***We estimate that just for the applicants' labs, more than 30 trainees will be using the Analyzer over the next 3 years. Importantly, the Seahorse analyzer is an instrument around which users interact to share knowledge, protocols and reagents, catalyzing networking and fostering new collaborations. Multi-parametric metabolic assessment of cellular basic functions is essential for achieving our proposed objectives. In the long term, the instruments will lead to better training and more comprehensive studies. Our findings may develop into innovative treatments for infections, autoimmunity, neurological diseases and cancers, which all have important socio-economic impact.***