Biparental haploid cells to generate genomically designed offspring

双亲单倍体细胞产生基因组设计的后代

基本信息

  • 批准号:
    536636-2018
  • 负责人:
  • 金额:
    $ 9.62万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Collaborative Research and Development Grants
  • 财政年份:
    2020
  • 资助国家:
    加拿大
  • 起止时间:
    2020-01-01 至 2021-12-31
  • 项目状态:
    已结题

项目摘要

In natural selection, differential fitness relies on the chromosomal shuffling during meiosis that enables the production of haploid gametes that are genetically different. However, meiotic-induced genetic variability can be an economic burden to the animal breeding industry since offspring often do not inherit the most desirable genes from their parents. Although genomic markers are currently being used to identify superior offspring at birth or even sooner, animals or embryos are frequently culled for not inheriting the best genes from their parents. This project is aimed at eliminating meiotic uncertainty from cattle breeding programs by determining the genomic value of both parental gametes before fertilization. Oocyte and sperm-derived haploid cells will be produced and analyzed to identify those carrying a superior genome to generate offspring. The project proposal is divided into 3 objectives: (1) Optimize the production of genomically characterized bovine haploid parthenogenetic (oocyte-derived) embryonic cells for use in diploidizations; (2) Develop protocols to multiply embryonic cell lines for obtaining unlimited quantities of haploid cells for diploidization; (3) Use genomically characterized andro- and parthenogenetic haploid cells to derive diploid embryos and viable offspring with biparental predetermined genomes. The proposal is financially and scientifically supported by SEMEX, a major industrial player in cattle genetics in Canada and internationally. The ability to predetermine the genome of offspring derived from genetically superior parents will provide SEMEX with a unique technical platform to incorporate into their selection programs and provide a considerable competitive advantage worldwide. Moreover, state-of-the-art scientific and technical exchanges between a postdoctoral fellow, a PhD student and SEMEX's research team is expected to contribute significantly to training of HQP in areas in high demand for Canada.
在自然选择中,差异适合度依赖于减数分裂期间的染色体改组,这使得能够产生遗传上不同的单倍体配子。然而,减数分裂诱导的遗传变异可能是动物育种业的经济负担,因为后代通常不能从其亲本遗传最理想的基因。尽管基因组标记目前被用于在出生时或甚至更早的时候识别上级后代,但动物或胚胎经常因为没有从父母那里继承最好的基因而被淘汰。该项目旨在通过在受精前确定双亲配子的基因组价值来消除牛育种程序中减数分裂的不确定性。将产生并分析卵母细胞和精子衍生的单倍体细胞,以鉴定携带上级基因组的细胞,从而产生后代。本项目建议书分为3个目标:(1)优化生产具有基因组特征的牛单倍体孤雌生殖(2)开发方案以使胚胎细胞系增殖以获得不限量的用于二倍体化的单倍体细胞;(3)使用基因组学表征的雄核和孤雌生殖单倍体细胞来获得具有双亲预定基因组的二倍体胚胎和存活后代。该提案得到了SEMEX的财政和科学支持,SEMEX是加拿大和国际上牛遗传学的主要工业参与者。预先确定来自遗传上级亲本的后代基因组的能力将为SEMEX提供一个独特的技术平台,以纳入其选择计划,并在全球范围内提供相当大的竞争优势。此外,博士后研究员,博士生和SEMEX研究团队之间的最先进的科学和技术交流预计将大大有助于加拿大高需求领域的HQP培训。

项目成果

期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Smith, Lawrence其他文献

Demands, resources, and work ability: A cross-national examination of health care workers
Cultivating Empathy Through Virtual Reality: Advancing Conversations About Racism, Inequity, and Climate in Medicine
  • DOI:
    10.1097/acm.0000000000003615
  • 发表时间:
    2020-12-01
  • 期刊:
  • 影响因子:
    7.4
  • 作者:
    Roswell, Robert O.;Cogburn, Courtney D.;Smith, Lawrence
  • 通讯作者:
    Smith, Lawrence
Supportive programs increase medical students' research interest and productivity
  • DOI:
    10.2310/6650.2006.05013
  • 发表时间:
    2006-05-01
  • 期刊:
  • 影响因子:
    2.6
  • 作者:
    Zier, Karen;Friedman, Erica;Smith, Lawrence
  • 通讯作者:
    Smith, Lawrence
Voxel Printing Anatomy: Design and Fabrication of Realistic, Presurgical Planning Models through Bitmap Printing
  • DOI:
    10.3791/63214
  • 发表时间:
    2022-02-01
  • 期刊:
  • 影响因子:
    1.2
  • 作者:
    Jacobson, Nicholas M.;Smith, Lawrence;MacCurdy, Robert
  • 通讯作者:
    MacCurdy, Robert
SHIFT SCHEDULE, WORK-FAMILY RELATIONSHIPS, MARITAL COMMUNICATION, JOB SATISFACTION AND HEALTH AMONG TRANSPORT SERVICE SHIFT WORKERS

Smith, Lawrence的其他文献

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{{ truncateString('Smith, Lawrence', 18)}}的其他基金

Nuclear-cytoplasmic interactions in mammalian embryos
哺乳动物胚胎中的核-细胞质相互作用
  • 批准号:
    RGPIN-2020-05278
  • 财政年份:
    2022
  • 资助金额:
    $ 9.62万
  • 项目类别:
    Discovery Grants Program - Individual
Biparental haploid cells to generate genomically designed offspring
双亲单倍体细胞产生基因组设计的后代
  • 批准号:
    536636-2018
  • 财政年份:
    2021
  • 资助金额:
    $ 9.62万
  • 项目类别:
    Collaborative Research and Development Grants
Nuclear-cytoplasmic interactions in mammalian embryos
哺乳动物胚胎中的核-细胞质相互作用
  • 批准号:
    RGPIN-2020-05278
  • 财政年份:
    2021
  • 资助金额:
    $ 9.62万
  • 项目类别:
    Discovery Grants Program - Individual
Nuclear-cytoplasmic interactions in mammalian embryos
哺乳动物胚胎中的核-细胞质相互作用
  • 批准号:
    RGPIN-2020-05278
  • 财政年份:
    2020
  • 资助金额:
    $ 9.62万
  • 项目类别:
    Discovery Grants Program - Individual
Nuclear-cytoplasmic interactions in mammalian embryos
哺乳动物胚胎中的核-细胞质相互作用
  • 批准号:
    RGPIN-2015-06228
  • 财政年份:
    2019
  • 资助金额:
    $ 9.62万
  • 项目类别:
    Discovery Grants Program - Individual
Biparental haploid cells to generate genomically designed offspring
双亲单倍体细胞产生基因组设计的后代
  • 批准号:
    536636-2018
  • 财政年份:
    2019
  • 资助金额:
    $ 9.62万
  • 项目类别:
    Collaborative Research and Development Grants
Nuclear-cytoplasmic interactions in mammalian embryos
哺乳动物胚胎中的核-细胞质相互作用
  • 批准号:
    RGPIN-2015-06228
  • 财政年份:
    2018
  • 资助金额:
    $ 9.62万
  • 项目类别:
    Discovery Grants Program - Individual
Use of haploid embryonic cells to generate offspring with predetermined genomes
使用单倍体胚胎细胞产生具有预定基因组的后代
  • 批准号:
    487107-2015
  • 财政年份:
    2017
  • 资助金额:
    $ 9.62万
  • 项目类别:
    Collaborative Research and Development Grants
Nuclear-cytoplasmic interactions in mammalian embryos
哺乳动物胚胎中的核-细胞质相互作用
  • 批准号:
    RGPIN-2015-06228
  • 财政年份:
    2017
  • 资助金额:
    $ 9.62万
  • 项目类别:
    Discovery Grants Program - Individual
Nuclear-cytoplasmic interactions in mammalian embryos
哺乳动物胚胎中的核-细胞质相互作用
  • 批准号:
    RGPIN-2015-06228
  • 财政年份:
    2016
  • 资助金额:
    $ 9.62万
  • 项目类别:
    Discovery Grants Program - Individual

相似国自然基金

果蝇Maternal Haploid 蛋白调控胚胎发育的分子机制研究
  • 批准号:
    31460299
  • 批准年份:
    2014
  • 资助金额:
    50.0 万元
  • 项目类别:
    地区科学基金项目

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Biparental haploid cells to generate genomically designed offspring
双亲单倍体细胞产生基因组设计的后代
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通过中心体复制“检查点”工程建立稳定的体细胞单倍体细胞
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