Use of lytic polysaccharide monooxygenases (LPMO) to enhance the enzyme treatment for cellulose nano-fibrils (CNF) preparation

使用溶解多糖单加氧酶（LPMO）增强纤维素纳米原纤维（CNF）制备的酶处理

• 批准号: 537196-2018
• 负责人: Ni, Yonghao
• 金额: $ 3.11万
• 依托单位: University of New Brunswick
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=699344
• 关键词: Use; lytic; polysaccharide; monooxygenases; LPMO

This collaborative research project will be undertaken by Dr. Yonghao Ni and his team at the University of New Brunswick, in partnership with Domtar Inc. Cellulases such as endoglucanase and exoglucanase have shown the ability to facilitate refining and fibrillation, and with a cellulose pretreatment step, allows the total energy consumption to be lowered in the production of nano-cellulose (eg. NFC). However the cellulose action is usually not specific in breaking the inter-fibril bonding/fibrillation, and fiber shortening is inevitable, particularly under strong cellulase treatment conditions which are required in the NFC production process. The overall strategy of the proposed research uses accessory enzymes/proteins such as lytic polysaccharide monooxygenases (LPMO) to boost cellulase performance, in particular favouring the specificity towards fibrillation. This will lead to the development of cost effective cellulase/accessory enzymes/proteins pretreatment technology to substantially decrease the energy consumption in NFC production. The Canadian pulp and paper industry faces strong international competition. The results from this research in relation to nanocellulose products will benefit not only Domtar, the industrial partner but also other Canadian pulp and paper companies. Details/deliverables include: 1) Investigation of the fibrillation response of bleached kraft pulp fibers to LPMO enhanced enzymatic treatment in terms of refining energy, width, length, aspect ratio and their distribution of the resultant cellulose nanofibrils (CNF), as well as mechanical strength properties of the CNF's with optimized process conditions. 2) Determine the fundamentals of LPMO enhanced enzymatic pretreatment of bleached kraft pulp fibers. 3) Develop novel, cost effective and environmentally friendly LPMO enhanced exnymatic processes for the production of high quality CNF product from bleached kraft pulps.

该合作研究项目将由新玩法大学倪永浩博士及其团队与Domtar Inc.合作开展。 纤维素酶如内切葡聚糖酶和外切葡聚糖酶已经显示出促进精制和原纤化的能力，并且在纤维素预处理步骤的情况下，允许在纳米纤维素的生产中降低总能量消耗（例如，纤维素酶）。NFC）。然而，纤维素作用通常不特异于破坏原纤间结合/原纤化，并且纤维缩短是不可避免的，特别是在NFC生产过程中所需的强纤维素酶处理条件下。 所提出的研究的总体策略使用辅助酶/蛋白质如溶解性多糖单加氧酶（LPMO）来提高纤维素酶的性能，特别是有利于对原纤化的特异性。 这将导致开发具有成本效益的纤维素酶/辅助酶/蛋白质预处理技术，以大幅降低NFC生产中的能耗。 加拿大纸浆和造纸业面临着强大的国际竞争。 这项与纳米纤维素产品相关的研究结果不仅将使工业合作伙伴Domtar受益，也将使其他加拿大纸浆和造纸公司受益。 详细信息/可交付成果包括：1）研究漂白硫酸盐浆纤维对LPMO增强的酶处理的原纤化响应，包括精制能量、所得纤维素纳米原纤（CNF）的宽度、长度、纵横比及其分布，以及具有优化工艺条件的CNF的机械强度性质。2)确定漂白硫酸盐浆纤维LPMO强化酶预处理的基本原理。3)开发新颖、经济、环保的LPMO强化脱硝工艺，从漂白硫酸盐浆中生产高质量的CNF产品。