Finding a needle in a genomic haystack: A tool for pinning down genetic edits

基因编辑这一迅速发展的学科已经通过一些更华丽或有争议的应用程序向公众介绍，包括克隆猛犸象或在出生前对人类24nome进行不必要的改变。 然而，在更主流的应用中，基因组编辑几乎进入了生物学研究和医学的方方面面，包括纠正毁灭性疾病的临床试验，使改变蚊子DNA来对抗疟疾寄生虫成为可能，并有可能帮助珊瑚提高对水温上升的抵抗力。随着基因编辑似乎无穷无尽的有益应用，对这些编辑的高效和高通量检测的商业需求已经发展起来。这种需求是基于基因编辑的低成功率，这导致实验室或公司需要筛选往往数百个样本来识别、选择或量化基因编辑。目前可用于确定哪些细胞受到基因组编辑影响的技术要么效率低下、劳动密集型、昂贵得令人望而却步，要么不利于工业和科学家所需的高通量能力。这项提议将为一种新的高通量和高效的基因分型奠定基础，称为混合熔体歧视(MMD)。

The rapidly expanding discipline of Genetic editing has been introduced to the general public through some of the flashier or controversial applications including efforts to clone the woolly mammoth or the unnecessary alteration of the human 24nome before birth. However, in more mainstream applications genomic editing has entered nearly every aspect of biology research and medicine including clinical trials to correct devastating diseases, enabled the altering of mosquitoes' DNA to combat the malaria parasite, and has the potential to help coral become more resistant to rising water temperatures. With seemingly endless beneficial applications for genetic editing, a commercial need has developed for efficient and high-throughput detection of these edits. This need is based on the low success rates of genetic edits that results in the need for labs or companies to screen often hundreds of samples to identify, select or quantify the genetic editing. Techniques currently available to determine which cells have been affected by genome editing are either inefficient, labor-intensive, prohibitively expensive, or are not conducive to high-throughput capabilities required by industry and scientists. This proposal will set the groundwork for a new high-throughput and efficient genotyping, called the Mixed Melt Discrimination (MMD).