Sensitive and specific electrochemical detection of COVID-19 (SARS-CoV2) based on isothermal amplification

基于等温扩增的 COVID-19 (SARS-CoV2) 的灵敏且特异的电化学检测

• 批准号: 554464-2020
• 负责人: Perreault, Jonathan
• 金额: $ 3.64万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=705425
• 关键词: Sensitive; specific; electrochemical; detection; COVID

The epidemic of the SARS-CoV2 virus (COVID-19) and its global spread urgently requires efficient means for rapid diagnosis, as well as detection in various environments (aside from clinical laboratories), such as airplanes, schools, potential animal carriers, etc. Indeed, most of the countries that appear to go through the current pandemic with currently relatively low death tolls and that are managing partial deconfinement are among those that are doing the most virus tests. We have previously devised a colorimetric method to detect specific virus DNA which could potentially be used for "on site" virus detection and which, in combination with an electrode-based detection device, would provide simple, sensitive and cheap on-site detection of the virus. SARS-CoV2 (the pathogen causing COVID-19) is an RNA virus, which adds a step for its amplification with the most commonly used detection methods, compared to other viruses with a genome made of DNA. The method we want to implement detects RNA and DNA in a similar way. Moreover, the current gold standard for virus detection requires specialized laboratory material and personnel, which also means sample transportation logistics and waiting queues for samples to be processed. We aim at implementing a novel DNA amplification method (called Rolling Circle Amplification) which does not rely on specialized equipment. By combining this with electrodes we expect to achieve sensitive and rapid detection of the virus genome without the need for bulky complex devices. Even if we all hope for a vaccine to come as soon as possible, there is still a dire need for an efficient, rapid and simple test to monitor, perhaps even on a daily or weekly basis, everyone who is going back to work or school to ensure that we can quickly respond to any new eclosion as soon as it can be detected. This would allow to have confinement measures targeted only on the workplaces with acknowledged COVID-19, but without affecting all work places and schools simultaneously.

SARS-CoV 2病毒的流行（COVID-19）及其全球传播迫切需要快速诊断以及在各种环境中检测的有效手段（除了临床实验室），如飞机，学校，潜在的动物携带者等。大多数似乎经历了当前流行病、目前死亡人数相对较低并正在设法部分解除隔离的国家，那些做病毒检测最多的人我们先前已经设计了一种比色法来检测特定的病毒DNA，该方法可以潜在地用于“现场”病毒检测，并且该方法与基于电极的检测装置相结合，将提供简单、灵敏和廉价的病毒现场检测。 SARS-CoV 2（引起COVID-19的病原体）是一种RNA病毒，与基因组由DNA组成的其他病毒相比，它增加了一个用最常用的检测方法进行扩增的步骤。我们想要实现的方法以类似的方式检测RNA和DNA。此外，目前病毒检测的黄金标准需要专门的实验室材料和人员，这也意味着样本运输物流和等待处理样本的排队。我们的目标是实现一种新的DNA扩增方法（称为滚环扩增），它不依赖于专门的设备。通过将其与电极相结合，我们期望实现对病毒基因组的灵敏和快速检测，而不需要庞大的复杂设备。 即使我们都希望疫苗能尽快问世，但仍然迫切需要一种高效、快速和简单的检测方法来监测，甚至可以每天或每周监测一次，监测每一个即将重返工作或学校的人，以确保我们能够在发现任何新的羽化时迅速作出反应。这将允许限制措施仅针对已确认患有COVID-19的工作场所，但不会同时影响所有工作场所和学校。