Electrochemical Therapy for Scar Treatments (Resubmission)

项目摘要 增生性瘢痕（HTS）导致严重的外形和功能丧失的深部分厚度 烧伤与烧伤后的发生率从33%到91%不等。HTS可以发生在任何 皮肤损伤，并与需要超过2-3周时间愈合的损伤相关， 特征在于由于异常过度的胶原蛋白生长而导致的增厚、宽和凸起的疤痕。如果疤痕 位于关节附近，由于皮肤挛缩，它们可能导致活动能力下降。为了解决这个 需要，我们正在开发一种基于氧化还原化学的低成本皮肤电化学疗法。通过 用微针电极在组织内产生酸/碱，ECT可以软化增厚的 胶原组织和低度细胞死亡，以触发正常而不是异常的再生 胶原结构如果ECT是有效的，它的实际优势是低成本，使用简单，和护理点 以相对较低的风险交付。我们假设电休克疗法可以产生“控制性”损伤， 受损的组织/胶原蛋白，从而取代增生性疤痕。我们拟议工作的目标是 建立ECT作为疤痕减少工具的可行性。首先，我们将制作和优化一个定制的 具有电位驱动电化学能力的ECT控制单元。其次，我们将确定安全和 使用离体猪样本进行有效的剂量测定。最后，我们将ECT的安全剂量范围应用于 增生性瘢痕模型。

Project Summary Hypertrophic scarring (HTS) leads to severe disfigurement and loss of function in deep partial thickness burn injuries with occurrence following burn injuries ranging from 33 to 91%. HTS can occur with any cutaneous injury and is associated with injuries that take longer than 2-3 weeks to heal and is characterized by thickened, wide, and raised scars due to abnormally excessive collagen growth. If scars are located near joints, they could lead to decreased mobility due to skin contractures. To address this need, we are developing a low-cost electrochemical therapy for skin based on redox chemistry. By producing acid/base within tissue with microneedle-electrodes, the ECT may soften the thickened collagen tissues and low-grade cellular death to trigger the regeneration of normal rather than aberrant collagen structure. If ECT is effective, its practical advantages are low-cost, simple use, and point of care delivery with relatively low risks. We hypothesize that the ECT can create ‘controlled’ injuries that remodel damaged tissue/collagen and thus replace hypertrophic scars. The goal of our proposed work is to establish the feasibility of ECT as a scar reduction tool. First, we will fabricate and optimize a custom ECT control unit with capability of potential driven electrochemistry. Second, we will identify safe and efficient dosimetry with ex vivo porcine specimens. Last, we will apply the safety dose range ECT to in vivo hypertrophic scar model.