Electrochemical Therapy for Scar Treatments (Resubmission)

项目摘要 增生性瘢痕(HTS)可导致深部增生性瘢痕严重毁容和功能丧失。 烧伤，烧伤后发生的比例从33%到91%不等。HTS可与任何 皮肤损伤，与需要2-3周以上愈合的损伤有关， 由于异常过度的胶原蛋白生长而导致的增厚、变宽和隆起的疤痕。如果伤疤 如果它们位于关节附近，则可能会因皮肤痉挛而导致行动不便。要解决这个问题 根据需要，我们正在开发一种基于氧化还原化学的低成本皮肤电化学疗法。通过 利用微针电极在组织内产生酸/碱，ECT可以软化增厚的组织 胶原蛋白组织和低度细胞死亡触发再生正常而不是异常 胶原蛋白结构。如果ECT是有效的，它的实际优势是低成本、简单使用和护理 风险相对较低的交付。我们假设ECT可以产生可控制的重塑损伤 破坏组织/胶原，从而取代增生性疤痕。我们提议的工作的目标是 确定ECT作为一种疤痕减少工具的可行性。首先，我们将构建和优化一个定制 具有电位驱动电化学能力的ECT控制单元。第二，我们将确定安全和 体外猪标本的有效剂量测定。最后，我们将把安全剂量范围ECT应用于In 活体增生性瘢痕模型。

Project Summary Hypertrophic scarring (HTS) leads to severe disfigurement and loss of function in deep partial thickness burn injuries with occurrence following burn injuries ranging from 33 to 91%. HTS can occur with any cutaneous injury and is associated with injuries that take longer than 2-3 weeks to heal and is characterized by thickened, wide, and raised scars due to abnormally excessive collagen growth. If scars are located near joints, they could lead to decreased mobility due to skin contractures. To address this need, we are developing a low-cost electrochemical therapy for skin based on redox chemistry. By producing acid/base within tissue with microneedle-electrodes, the ECT may soften the thickened collagen tissues and low-grade cellular death to trigger the regeneration of normal rather than aberrant collagen structure. If ECT is effective, its practical advantages are low-cost, simple use, and point of care delivery with relatively low risks. We hypothesize that the ECT can create ‘controlled’ injuries that remodel damaged tissue/collagen and thus replace hypertrophic scars. The goal of our proposed work is to establish the feasibility of ECT as a scar reduction tool. First, we will fabricate and optimize a custom ECT control unit with capability of potential driven electrochemistry. Second, we will identify safe and efficient dosimetry with ex vivo porcine specimens. Last, we will apply the safety dose range ECT to in vivo hypertrophic scar model.