Characterization of the RNA-protein interactions involved in the replication of Subviral RNAs

亚病毒 RNA 复制中涉及的 RNA-蛋白质相互作用的表征

• 批准号: RGPIN-2015-04888
• 负责人: Pelchat, Martin
• 金额: $ 2.19万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=707952
• 关键词: Characterization; RNA; protein; interactions; involved

Plant viroids and the hepatitis delta virus (HDV) have very small RNA genomes and rely solely on interaction with host proteins for their life cycle. To better understand these subviral RNA species and how RNA-based life forms replicate, my long term objective is to identify both the host proteins and the RNA motifs involved in their biology. To achieve this goal, my research program will use three integrative approaches: 1) Using Molecular Biology, I will identify host proteins used by one viroid (i.e. potato spindle tuber viroid; PSTVd) and HDV, and characterize the RNA motifs they bind. 2) Using Genetics and Bioinformatics, I will identify the genomic RNA features that are conserved and selected for during the replication of several subviral RNA species. 3) Using Evolutionary genetics, I will select a small RNA replicon derived from the HDV RNA genome to better understand RNA-based life forms. By integrating these approaches, my multidisciplinary research program will allow to understand the biology of these unique RNA-based life forms. These findings and approaches will also be valuable for comprehensive investigations of RNA-protein interactions in other biological processes, and will have broad implications for studying the structure-function relationships of RNA molecules.

植物类病毒和丁型肝炎病毒（HDV）的RNA基因组非常小，它们的生命周期完全依赖于与宿主蛋白的相互作用。为了更好地了解这些亚病毒RNA物种以及基于RNA的生命形式如何复制，我的长期目标是识别宿主蛋白质和参与其生物学的RNA基序。为了实现这一目标，我的研究计划将使用三种综合方法：