Understanding the role of host genetics in regulating the bovine gut microbiome

了解宿主遗传学在调节牛肠道微生物组中的作用

• 批准号: RGPIN-2019-05594
• 负责人: Guan, Leluo
• 金额: $ 4.74万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=715174
• 关键词: Understanding; role; host; genetics; regulating

Beef is a highly nutritious component of the diet providing readily accessible protein and essential micronutrients in a relatively small serving. In many parts of the world significant parts of the population is undernourished, even in Canada. Beef cattle also make use of land resources that are unsuitable or inefficient for producing crops. Thus, in many nations, ruminant protein is an integral component of the human diet and its production has contributed to the development and elimination of poverty in human society. However, ruminants are seen as contributing significantly to global warming. This aspect can be mitigated by selecting for improved feed efficiency or conversion of forage to meat. Improving feed efficiency of cattle is one of the grand challenges for industry and society in order to meet increased human consumer's demand but also to lower production cost and to reduce the amount of greenhouse gas produced per kg of product as well as the manure waste. To date, the biological processes underlying cattle feed efficiency and methane production in the ruminants are still largely unknown. Past studies from my laboratory have revealed that the composition and activity of the rumen microbiota in cattle with high feed efficiency differs from those with low feed efficiency. Ruminants have evolved to possess a diverse population of microbes in their rumen that enable them to convert forage into nutrients for animal growth. This symbiotic microbiota can degrade most complex plant polysaccharides to produce short chain fatty acids, proteins, and vitamins, which provide satisfy the animal's energy requirement for maintenance and growth. This suggests that “rumen fermentation”, as the starting point of nutrient digestion, is a major biological process determining individual feed efficiency. Although diet is a key factor for the rumen microbiome, recent research has highlighted that the microbiome can vary widely between animals fed the same diet in the same environment. Currently, we do not understand what impacts this aspect. This project seeks to increase our understanding of rumen function and how it is impacted by host genetics in order to improve its function and efficiency. We will ask the following questions: 1) Does the host genotype partially explain the variation in rumen microbial communities in feedlot cattle exhibiting differences in feed efficiency? 2). Can the microbiome of the lower gut of cattle also contribute to this variation and how? E.g. by impacting host gene expression and amino acid absorption and/or the host impacting microbial interactions and function in the lower gut? An understanding of this interaction can then form the basis of new approaches to manipulate it to help achieve more sustainable or environment friendly cattle production, the ultimate goal of my research program.

牛肉是饮食中营养丰富的组成部分，提供容易获得的蛋白质和相对少量的必需微量营养素。在世界许多地方，甚至在加拿大，也有相当一部分人口营养不良。肉牛还利用不适合或效率不高的土地资源生产作物。因此，在许多国家，反刍动物蛋白是人类饮食的组成部分，其生产有助于人类社会的发展和消除贫困。然而，反刍动物被认为对全球变暖有重大贡献。这方面可以通过选择提高饲料效率或将饲料转化为肉类来缓解。提高牛的饲料效率是工业和社会面临的重大挑战之一，以满足日益增长的人类消费者需求，同时降低生产成本，减少每千克产品产生的温室气体量以及粪便废物。迄今为止，牛饲料效率和反刍动物甲烷产生的生物学过程在很大程度上仍然未知。我实验室过去的研究表明，高饲料效率牛的瘤胃微生物群的组成和活性与低饲料效率牛不同。反刍动物已经进化到在它们的瘤胃中拥有多样化的微生物群体，使它们能够将饲料转化为动物生长的营养物质。这种共生微生物群可以降解大多数复杂的植物多糖，产生短链脂肪酸，蛋白质和维生素，满足动物维持和生长的能量需求。这表明，“瘤胃发酵”作为营养物质消化的起点，是决定个体饲料效率的主要生物学过程。虽然饮食是瘤胃微生物组的关键因素，但最近的研究强调，在相同环境中饲喂相同饮食的动物之间的微生物组可能存在很大差异。目前，我们还不清楚这方面的影响。该项目旨在增加我们对瘤胃功能的了解，以及它如何受到宿主遗传学的影响，以提高其功能和效率。我们将提出以下问题：1）宿主基因型是否部分解释了饲养场牛中表现出饲料效率差异的瘤胃微生物群落的变化？2）的情况。牛下消化道的微生物组也会导致这种变异吗？例如，通过影响宿主基因表达和氨基酸吸收和/或宿主影响下消化道中的微生物相互作用和功能？对这种相互作用的理解可以形成新方法的基础，以帮助实现更可持续或环境友好的养牛生产，这是我研究计划的最终目标。