Microfluidic Sample Handling for the Spectroscopic Integration (SPIN) Program

用于光谱积分 (SPIN) 计划的微流体样品处理

基本信息

  • 批准号:
    RGPIN-2020-05371
  • 负责人:
  • 金额:
    $ 2.84万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2020
  • 资助国家:
    加拿大
  • 起止时间:
    2020-01-01 至 2021-12-31
  • 项目状态:
    已结题

项目摘要

Long Term Vision To develop a microfluidic platform to integrate distinct single cell spectroscopic technologies for the study of heterogeneous cell populations with single cell granularity across an unprecedented spectral range. The Full SPectrum INterrogation Project (Full SPIN) is an initiative to incorporate multiple spectral modalities in order to gain a complete picture of dynamics within heterogeneous cell populations with single cell granularity. Full SPIN take disaggregated cell samples, image the cells, and then characterize single cell transmission spectra using four complementary technologies: Radio Frequency spectroscopy, Terahertz spectroscopy, Raman spectroscopy, and (cavity-reflection-enhanced) Visible spectroscopy. This system will allow for the cell-indexed correlation of the spectra at a single cell level so that their contribution to the bulk response can be inferred and the heterogeneous nature of the population can be better understood. The proposed work spans the fabrication of microfluidic modules with optimized materials for each spectroscopic technology, the cell handling needed to index a library of cells so that the spectra can be correlated at the single cell level across all the interrogation wavelengths, and the demonstration of the platform's utility by studying the cell population's response to stimulus. The work supported in this proposal will involve 2 PhD, 1 MASc and 2 undergraduate students per year for the duration of the funding. The HQP will be focused on the development and demonstration of the various modules that make up the Full SPIN platform. Human samples will not be used in the work proposed, which will focus on technology development and use standard cell lines to demonstrate the functionality and utility of the platform. The modular platform consists of several components: an indexing chip to generate a library of single cells stored in a water-in-oil emulsion, an index cartridge that will store the library and maintain cell viability, and a set of interrogation chips customized to each of the spectral interrogation methods. The library of cells will be transferred in the index cartridge to each interrogation chip in turn and a second index cartridge will collect the library again at the output of the interrogation chip and then transfer the library to the next interrogation module in turn. The final milestone for the proposed work is processing an indexed cell library of >1000 cells, imaging the cells, and acquiring correlated spectra in all of the spectral windows, both before and after a stimulus event. The resulting data will then be used to enable the statistical modelling of the individual cellular contribution population's response to the stimuli. The applications of this technology platform are broad, answering basic questions about heterogeneous cellular response.
长期愿景开发一个微流控平台,整合独特的单细胞光谱技术,用于在前所未有的光谱范围内研究具有单细胞粒度的异质细胞群。 全光谱询问项目(Full SPIN)是一项将多种光谱模式结合起来的倡议,目的是获得具有单细胞粒度的异质细胞群内动力学的完整图像。Full SPIN采集分解的细胞样本,对细胞进行成像,然后使用四种互补技术表征单细胞透射光谱:射频光谱、太赫兹光谱、拉曼光谱和(腔反射增强)可见光谱。该系统将允许在单个细胞水平上的光谱的细胞索引相关性,以便可以推断它们对批量响应的贡献,并且可以更好地理解群体的异质性。拟议的工作涵盖了微流控模块的制造,每个光谱技术的优化材料,索引细胞库所需的细胞处理,以便光谱可以在所有询问波长的单细胞水平上相互关联,以及通过研究细胞群体对刺激的反应来展示平台的实用性。 这项提案中支持的工作将涉及2博士,1硕士和2本科生每年的资助期间。HQP将专注于开发和演示构成完整SPIN平台的各种模块。 拟议的工作将不使用人类样本,该工作将侧重于技术开发,并使用标准细胞系来证明平台的功能和实用性。 模块化平台由几个组件组成:索引芯片,用于生成存储在油包水乳液中的单细胞库;索引盒,用于存储库并保持细胞活力;以及一组针对每种光谱询问方法定制的询问芯片。 细胞库将在索引盒中依次转移到每个询问芯片,并且第二索引盒将在询问芯片的输出处再次收集库,然后依次将库转移到下一个询问模块。 所提出的工作的最后一个里程碑是处理>1000个细胞的索引细胞库,对细胞进行成像,并在刺激事件之前和之后在所有光谱窗口中获取相关光谱。 然后将使用所得数据来实现个体细胞贡献群体对刺激的响应的统计建模。 该技术平台的应用非常广泛,可以回答有关异质细胞反应的基本问题。

项目成果

期刊论文数量(0)
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Foulds, Ian其他文献

The Pseudomonas aeruginosa generalized transducing phage φPA3 is a new member of the φKZ-like group of 'jumbo' phages, and infects model laboratory strains and clinical isolates from cystic fibrosis patients
  • DOI:
    10.1099/mic.0.044701-0
  • 发表时间:
    2011-03-01
  • 期刊:
  • 影响因子:
    2.8
  • 作者:
    Monson, Rita;Foulds, Ian;Salmond, George P. C.
  • 通讯作者:
    Salmond, George P. C.

Foulds, Ian的其他文献

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{{ truncateString('Foulds, Ian', 18)}}的其他基金

Microfluidic Sample Handling for the Spectroscopic Integration (SPIN) Program
用于光谱积分 (SPIN) 计划的微流体样品处理
  • 批准号:
    RGPIN-2020-05371
  • 财政年份:
    2022
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Microfluidic Sample Handling for the Spectroscopic Integration (SPIN) Program
用于光谱积分 (SPIN) 计划的微流体样品处理
  • 批准号:
    RGPIN-2020-05371
  • 财政年份:
    2021
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
High Throughput Microfluidics
高通量微流控
  • 批准号:
    RGPIN-2015-06711
  • 财政年份:
    2019
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
High Throughput Microfluidics
高通量微流控
  • 批准号:
    RGPIN-2015-06711
  • 财政年份:
    2018
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
High Throughput Microfluidics
高通量微流控
  • 批准号:
    RGPIN-2015-06711
  • 财政年份:
    2017
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
High Throughput Microfluidics
高通量微流控
  • 批准号:
    RGPIN-2015-06711
  • 财政年份:
    2016
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
High Throughput Microfluidics
高通量微流控
  • 批准号:
    RGPIN-2015-06711
  • 财政年份:
    2015
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Nanotechnology prototyping platform
纳米技术原型平台
  • 批准号:
    343745-2007
  • 财政年份:
    2008
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Postdoctoral Fellowships
Nanotechnology prototyping platform
纳米技术原型平台
  • 批准号:
    343745-2007
  • 财政年份:
    2007
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Postdoctoral Fellowships
ThermalMicroStereoLithography Capable of Freely Moving Parts
能够自由移动部件的热微立体光刻技术
  • 批准号:
    318084-2005
  • 财政年份:
    2006
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Postgraduate Scholarships - Doctoral

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Microfluidic Sample Handling for the Spectroscopic Integration (SPIN) Program
用于光谱积分 (SPIN) 计划的微流体样品处理
  • 批准号:
    RGPIN-2020-05371
  • 财政年份:
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  • 项目类别:
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Microfluidic Sample Handling for the Spectroscopic Integration (SPIN) Program
用于光谱积分 (SPIN) 计划的微流体样品处理
  • 批准号:
    RGPIN-2020-05371
  • 财政年份:
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