A portable DIHM for in-situ detection of airborne viruses

用于原位检测空气传播病毒的便携式 DIHM

• 批准号: RTI-2021-00793
• 负责人: Ariya, Parisa
• 金额: $ 3.87万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=717689
• 关键词: portable; DIHM; situ; detection; airborne

A portable Digital In-line holographic microscopy is requested to enable us to perform a medium-risk high-impact potential research project to revolutionize the detection of microbes, in moving matrices such as air and water. In the era of COVID-19 and the climate change, novel mutating viruses will increasingly affect humans. Yet, the humanity is not ready for them. Even key identified factors in viral disease transmission of respiratory diseases, such as size, number density and transformation processes of viruses, in their entirety, in real-time or in-situ are unknown that are essential for formulating smart responses and better management of the current and future pandemics. There is an urgent need for this equipment. We have recently developed a hybrid technology (nano-DIHM) which consists of recently McGill invented airborne interface and a DIHM unit, that was borrowed. We have shown that the developed nano-DIHM is capable of in-situ and real-time rapid detection (seconds) of viruses. We have also shown that this technique is capable of observing the entire microbes, in a dynamic medium such as ambient air, water, or indoor air in buildings. To our knowledge, it will be the first dynamic in-situ and real-time detection acapability for airborne virus. The existing techniques for airborne viruses are mostly collection (i.e., not real-time or in-situ) or lack selectivity. This technology is promising, as our preliminary data show that this technique has high selectivity. Yet, we have to return the DIHM, and we urgently need to have a dedicated unit to be couple of our inventions, to enable to contribute to science. We have three innovative research objectives for next 5 years: (1) Creation of metadata for bioaerosols including airborne viruses; (2) Machine learning (artificial intelligent and data mining techniques) to further improve the quality of metadata and enhance detection AND identification of bioaerosols (such as virus) in air, which could have never been done before, to our knowledge., smart Nano-DIHM which is Automated with remote sensing capability. This novel technology will allow to contribute to key information on (a) rapid identification, (b) number density, (c) transformation, (c) hot spot identification and (d) viral transmission process, by in-situ real time imaging of particle phase(s), heterogeneity and transformation of surfaces. By addressing these objectives, we will be able to observe, analyze and identify the data imaging dynamically, in air, in real-time, with the option of remote sensing. We will be able to create a novel technological capability for airborne viruses, in Canada and globally. An average of 4 bright HQP per year will be trained on this RTI equipment, in an equitable, diverse and intellectually exciting laboratory environment, that foster creations and innovations, while exploring the boundaries of unknown.

要求便携式数字全息显微镜使我们能够执行中等风险的高影响力潜在的研究项目，以彻底改变微生物的检测，例如空气和水。在Covid-19的时代和气候变化时代，新型的突变病毒将日益影响人类。但是，人类还没有为他们准备。即使是关键的呼吸道疾病传播中的因素，例如大小，数量密度和病毒的转化过程，全部，实时或原位是未知的，对于对当前和未来大流行的更好地进行制定，这对于制定智能反应以及更好地管理。 迫切需要此设备。我们最近开发了一种混合技术（Nano-DIHM），该技术由最近麦吉尔发明了空中界面和一个借用的DIHM单元。我们已经表明，发达的纳米-DIHM能够对病毒的原位和实时快速检测（秒）。我们还表明，该技术能够在建筑物中的环境空气，水或室内空气等动态介质中观察整个微生物。 据我们所知，这将是空气传播病毒的第一个动态原位和实时检测可示置性。 空气传播病毒的现有技术主要是收集（即，不是实时或原位）或缺乏选择性。这项技术是有希望的，因为我们的初步数据表明，该技术具有很高的选择性。但是，我们必须退还DIHM，我们迫切需要拥有一个专门的单位才能成为我们的发明，以便为科学做出贡献。 接下来的5年我们将拥有三个创新的研究目标： （1）为包括空气传播病毒在内的生物溶质的元数据创建； （2）机器学习（人工智能和数据挖掘技术）进一步提高元数据的质量，并增强对空气中生物溶质（例如病毒）的检测和鉴定，据我们所知，这是从未做过的。 具有遥感能力自动化的智能纳米二iHm。 这项新型技术将允许有关（a）快速识别，（b）数量密度，（c）转换，（c）热点识别和（d）病毒传播过程的关键信息，通过对粒子相，异质性和表面转换的现场实时成像。通过解决这些目标，我们将能够在空中，实时，实时地观察，分析和识别数据成像，并选择遥感。在加拿大和全球，我们将能够为机载病毒创建一种新颖的技术能力。 平均每年4个明亮的HQP将在此RTI设备上进行培训，该设备在一个公平，多样化和智力上令人兴奋的实验室环境中，促进创造和创新，同时探索未知的界限。