A portable DIHM for in-situ detection of airborne viruses

用于原位检测空气传播病毒的便携式 DIHM

• 批准号: RTI-2021-00793
• 负责人: Ariya, Parisa
• 金额: $ 3.87万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=717689
• 关键词: portable; DIHM; situ; detection; airborne

A portable Digital In-line holographic microscopy is requested to enable us to perform a medium-risk high-impact potential research project to revolutionize the detection of microbes, in moving matrices such as air and water. In the era of COVID-19 and the climate change, novel mutating viruses will increasingly affect humans. Yet, the humanity is not ready for them. Even key identified factors in viral disease transmission of respiratory diseases, such as size, number density and transformation processes of viruses, in their entirety, in real-time or in-situ are unknown that are essential for formulating smart responses and better management of the current and future pandemics. There is an urgent need for this equipment. We have recently developed a hybrid technology (nano-DIHM) which consists of recently McGill invented airborne interface and a DIHM unit, that was borrowed. We have shown that the developed nano-DIHM is capable of in-situ and real-time rapid detection (seconds) of viruses. We have also shown that this technique is capable of observing the entire microbes, in a dynamic medium such as ambient air, water, or indoor air in buildings. To our knowledge, it will be the first dynamic in-situ and real-time detection acapability for airborne virus. The existing techniques for airborne viruses are mostly collection (i.e., not real-time or in-situ) or lack selectivity. This technology is promising, as our preliminary data show that this technique has high selectivity. Yet, we have to return the DIHM, and we urgently need to have a dedicated unit to be couple of our inventions, to enable to contribute to science. We have three innovative research objectives for next 5 years: (1) Creation of metadata for bioaerosols including airborne viruses; (2) Machine learning (artificial intelligent and data mining techniques) to further improve the quality of metadata and enhance detection AND identification of bioaerosols (such as virus) in air, which could have never been done before, to our knowledge., smart Nano-DIHM which is Automated with remote sensing capability. This novel technology will allow to contribute to key information on (a) rapid identification, (b) number density, (c) transformation, (c) hot spot identification and (d) viral transmission process, by in-situ real time imaging of particle phase(s), heterogeneity and transformation of surfaces. By addressing these objectives, we will be able to observe, analyze and identify the data imaging dynamically, in air, in real-time, with the option of remote sensing. We will be able to create a novel technological capability for airborne viruses, in Canada and globally. An average of 4 bright HQP per year will be trained on this RTI equipment, in an equitable, diverse and intellectually exciting laboratory environment, that foster creations and innovations, while exploring the boundaries of unknown.

需要便携式数字同轴全息显微镜，以使我们能够执行中等风险、高影响潜力的研究项目，以革命性地检测空气和水等移动基质中的微生物。在新冠肺炎和气候变化的时代，新型变异病毒将越来越多地影响人类。然而，人类还没有为他们做好准备。甚至在呼吸道疾病的病毒传播中已确定的关键因素，如病毒的整体、实时或就地传播的大小、数量密度和转化过程，也是未知的，这些因素对于制定明智的应对措施和更好地管理当前和未来的大流行病至关重要。 对这种设备的需求非常迫切。我们最近开发了一种混合技术(Nano-Dihm)，它由麦吉尔最近发明的机载接口和借用的Dihm单元组成。我们已经证明，所开发的纳米Dihm能够对病毒进行原位和实时快速检测(秒)。我们还证明了这项技术能够观察到动态介质中的整个微生物，例如环境空气、水或建筑物的室内空气。据我们所知，这将是第一个动态、原位和实时检测空气传播病毒的能力。现有的空气传播病毒检测技术大多是采集(即非实时或就地)或缺乏选择性。这项技术很有前景，因为我们的初步数据表明，这项技术具有很高的选择性。然而，我们必须归还Dihm，我们迫切需要一个专门的单位来配合我们的发明，使之能够为科学做出贡献。我们为未来5年制定了三个创新研究目标： (1)创建包括空气传播病毒在内的生物气溶胶的元数据； (2)机器学习(人工智能和数据挖掘技术)，以进一步提高元数据的质量，并加强对空气中生物气溶胶(如病毒)的检测和识别，据我们所知，这是以前从未做过的。 智能Nano-Dihm是自动化的，具有遥感能力。 这项新技术将有助于(A)快速识别、(B)数密度、(C)转变、(C)热点识别和(D)病毒传播过程的关键信息，通过粒子相的原位实时成像(S)、表面的异质性和转变。通过解决这些目标，我们将能够通过遥感选项在空中实时动态地观察、分析和识别数据成像。我们将能够在加拿大和全球创造一种针对空气传播病毒的新技术能力。平均每年将有4名聪明的HQP在这种RTI设备上接受培训，在一个公平、多样化和令人兴奋的实验室环境中，在探索未知边界的同时促进创造和创新。