Identifying putative bioactive redox properties of maillard reaction products

识别美拉德反应产物的假定生物活性氧化还原特性

• 批准号: RGPIN-2019-04885
• 负责人: Kitts, David
• 金额: $ 4.74万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=737925
• 关键词: Identifying; putative; bioactive; redox; properties

The Maillard reaction (MR) is considered one of the most important chemical reactions occurring in processed foods. Traditionally, attention on MR has been given to sensory qualities of food that include color, flavor and texture and also effects on protein quality. However, recent evidence suggests that specific components of MR products formed during intermediate, or later stages, contribute to important antioxidant and anti-inflammatory activities which can influence intestinal health and function. I am interested in expanding our knowledge on the impact of thermal processing and non-thermal technologies that generate defined MR products with potential to act as promoters of intestinal natural defenses. Little effort has been given to isolate active components of MR that contribute to the redox status of the small intestine and will affect both morphology and function. My laboratory has previously demonstrated various antioxidant and anti-inflammatory activities of MR products derived from sugar-amino acid model systems in chemical and intestinal cell-based assays. I hypothesize that MR bioactives, once extracted from MR Mixtures (MRM), can impact on intestinal redox biology. These compounds, or mixtures, exhibit capacities to mitigate oxidative stress by directly scavenging radicals as well as regulating signaling pathways that impinge on normal redox responses. This work will unlock a new mechanistic concept on the involvement of MR with cellular oxidants in maintaining intestinal function. My research program will be active to isolate MR products formed in both model and intact food systems that exhibit antioxidant and anti-inflammatory bioactivity. I will study underlying mechanism(s) that focus on molecular interactions of Nfr2 signaling in Caco2 cells and the upregulation of genes involved in the natural defense of oxidative stress. Knowledge gained therein will lead to further experiments related to tight junction protein (TJP) morphology, which govern structural and functional integrity of the intestinal barrier. Significance: Xenobiotic agents that share in the capacity to elicit oxidative stress lead to intestinal dysfunction; the consequences of which are nutrient malabsorption and exposure to antigens or pathogens derived from the diet. The daily dietary intake of MR products from food systems is known to be significant, but chemoprotective potential of bioactive MR components in maintaining gut health through interactions with redox biology is not known. My program will make use of leading technologies on Maillard chemistry and intestinal cell culture models to address questions on the bioactive significance of MR products. New hypothesis will be tested and fundamental knowledge will be gained on underlying mechanisms where MRM can protect the intestine from oxidative stress. This will lead to practical applications for the food industry and will have benefits for the consumer.

美拉德反应（MR）被认为是发生在加工食品中最重要的化学反应之一。传统上，对核磁共振的关注主要集中在食物的感官质量上，包括颜色、味道和质地，以及对蛋白质质量的影响。然而，最近的证据表明，在中期或后期形成的MR产品的特定成分有助于重要的抗氧化和抗炎活性，从而影响肠道健康和功能。我有兴趣扩大我们对热加工和非热技术的影响的知识，这些技术产生的MR产品具有作为肠道自然防御促进剂的潜力。很少有人努力分离MR的活性成分，这些活性成分有助于小肠的氧化还原状态，并将影响形态和功能。我的实验室之前已经在化学和肠细胞分析中证明了来自糖-氨基酸模型系统的MR产品的各种抗氧化和抗炎活性。我假设磁共振生物活性物质，一旦从磁共振混合物（MRM）中提取，可以影响肠道氧化还原生物学。这些化合物或混合物通过直接清除自由基以及调节影响正常氧化还原反应的信号通路，表现出减轻氧化应激的能力。这项工作将揭开MR与细胞氧化剂参与维持肠道功能的新机制概念。我的研究项目将积极分离模型和完整食品系统中形成的具有抗氧化和抗炎生物活性的MR产品。我将研究潜在的机制，重点是Nfr2信号在Caco2细胞中的分子相互作用和参与氧化应激自然防御的基因上调。在此获得的知识将导致与紧密连接蛋白（TJP）形态学相关的进一步实验，TJP控制肠屏障的结构和功能完整性。意义：参与引起氧化应激的外源性药物可导致肠道功能障碍；其后果是营养吸收不良和暴露于来自饮食的抗原或病原体。已知每日从食物系统中摄入MR产品是重要的，但生物活性MR成分通过与氧化还原生物学相互作用来维持肠道健康的化学保护潜力尚不清楚。我的项目将利用美拉德化学和肠道细胞培养模型的领先技术来解决MR产品的生物活性意义问题。新的假设将被测试，并将获得关于MRM保护肠道免受氧化应激的潜在机制的基本知识。这将导致食品工业的实际应用，并将有利于消费者。