Intensity Fluctuations in Single Molecule Surface-Enhanced Raman Scattering
单分子表面增强拉曼散射中的强度波动
基本信息
- 批准号:RGPIN-2020-04236
- 负责人:
- 金额:$ 5.76万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2021
- 资助国家:加拿大
- 起止时间:2021-01-01 至 2022-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Surface-enhanced Raman scattering (SERS) is a highly sensitive vibrational spectroscopy technique capable of providing unique molecular fingerprinting information. SERS is observed for molecules adsorbed on certain areas of nanostructured metallic surfaces known as SERS hotspots. The tightly focussed light at the hotspots allows even the detection of single individual molecules by Raman scattering. Experimentally, the single molecule signature consists of strong time- (or space-) dependent SERS intensity fluctuations (SIFs). Although the phenomenon of single-molecule SERS (SM-SERS) has been demonstrated more than 20 years ago, there were very few examples of actual uses of SM-SERS to either acquire fundamental information about the hotspot environment or for chemical applications, such as in analytical chemistry. The long term goal of our Discovery program is to lead the SERS field to a new direction that takes into consideration the statistics of single particle, single molecule and even single photon events. This new approach will lead to several interesting new insights and potential new applications. In order to achieve this long term goal, we propose the application of a new technology for the study of SIFs with high spatial and time resolution. In the short term, this new method will be used to follow dynamic processes at the hotspot that have remained hidden due to the notorious low acquisition rate (~ 1 Hz) typically used in a regular SERS measurements. Experiments will be realized at different temperatures, allowing, for instance, the quantification of the energetics of fundamental processes, such as metallic cluster reconstruction at the hotspot area. A systematic investigation of the role of several factors, such as the nature of the metal, the geometric characteristics of the nanometric environment at the hotspot region, and the molecular affinity to the metallic surface, will be investigated. The overall goal in the short term will be to extract fundamental information about the metal-molecule interaction at the hotspot that was not accessible before. The analysis of the frequencies of the fast fluctuations will be used to generate an alternative approach in SERS quantification. Although SERS is a very sensitive method, its application in analytical chemistry has been hampered by intrinsic intensity fluctuations that translate into large errors in analytical calibration curves. We suggest that, for very low concentrations, SIF frequencies might correlate well (less variations) with the concentration of a given analyte in solution. Finally, we will be able to combine SM-SERS measurements with high-speed acquisition to investigate single photons emitted by single molecules.
表面增强拉曼散射(Sers)是一种高灵敏度的振动光谱技术,能够提供独特的分子指纹信息。Sers被观察到吸附在纳米结构金属表面的某些区域上的分子,称为Sers热点。在热点处紧密聚焦的光甚至允许通过拉曼散射检测单个分子。实验上,单分子签名由强烈的时间(或空间)依赖的Sers强度波动(SIF)组成。虽然单分子Sers(SM-SERS)现象在20多年前就已被证实,但实际使用SM-SERS来获取有关热点环境的基本信息或用于化学应用(例如分析化学)的例子很少。 我们发现计划的长期目标是将Sers领域引向一个新的方向,考虑到单粒子,单分子甚至单光子事件的统计。这种新方法将带来一些有趣的新见解和潜在的新应用。为了实现这一长期目标,我们提出了一种新的技术,用于研究具有高空间和时间分辨率的应力强度因子的应用。在短期内,这种新方法将用于跟踪热点处的动态过程,由于常规Sers测量中通常使用的臭名昭著的低采集速率(~ 1 Hz),这些热点一直处于隐藏状态。实验将在不同的温度下实现,例如,允许基本过程的能量学的量化,例如在热点区域的金属簇重构。系统的调查的几个因素的作用,如金属的性质,在热点区域的纳米环境的几何特征,和金属表面的分子亲和力,将进行调查。短期内的总体目标将是提取以前无法获得的关于热点处金属分子相互作用的基本信息。 快速波动的频率的分析将用于生成Sers量化的替代方法。虽然Sers是一种非常灵敏的方法,但其在分析化学中的应用受到固有强度波动的阻碍,这些波动转化为分析校准曲线中的大误差。我们认为,对于非常低的浓度,SIF频率可能与溶液中给定分析物的浓度相关(变化较小)。最后,我们将能够将联合收割机SM-SERS测量与高速采集相结合,以研究单分子发射的单光子。
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
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Brolo, Alexandre其他文献
Brolo, Alexandre的其他文献
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