Determination and optimization of Bacillus subtilis traits required for its establishment, persistence and activities on plant roots

枯草芽孢杆菌在植物根部建立、持久性和活性所需性状的确定和优化

• 批准号: RGPIN-2020-07057
• 负责人: Beauregard, Pascale
• 金额: $ 2.62万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=744991
• 关键词: Determination; optimization; Bacillus; subtilis; traits

Microorganisms are essential for the good health of biotic ecosystems. Thus, use of plant-beneficial bacteria as organic amendments for increase growth and pathogen protection in agriculture is a promising alternative to the current over-usage of chemicals. While many of these products are already on the market, inconsistence in their efficacy is a major hurdle to their widespread usage in fields and greenhouses. Bacteria from the Bacillus genera, and in particular of the subtilis and velezensis species, are of central interest in the development of biological solutions in agriculture. These bacteria possess many plant growth-promoting (PGP) traits, such as direct stimulation of the metabolism of the plant, and aid for the plant to acquire diverse nutrients. Most Bacillus species secrete an arsenal of antimicrobials and favor the plant resistance's toward pathogen infections, thus acting as natural, biological pesticides and fungicides. Bacillus species can also form resistant endospores, a dormant state ideal for product formulation. Fundamental questions about the interaction between beneficial Bacillus and plants are still unanswered, which slows down our capacity to formulate consistently efficient products. In this research program, we will explore three fundamental questions with the aim of better understanding the complex interactions between Bacillus species and plants. First, we will determine what are the environmental conditions that modulate dormancy of Bacillus on roots. In the same objective, we will also evaluate if Bacillus spores can still exert beneficial effects on plants. Secondly, we will identify what are Bacillus cellular components that are critical for the establishment and persistence on roots in absence and in presence of competing strains. Finally, we will evaluate to what extent Bacillus beneficial activities are expressed when it is on plant roots, and how to potentiate their activities in natural conditions. This research program will lead to a fundamental comprehension of in planta interactions of B. subtilis and plants, providing key information for the future development, usage and formulation of this biological growth promoter and pesticide. These knowledge are very important, since they are necessary to develop and implant sustainable agricultural systems.

微生物对生物生态系统的健康至关重要。因此，在农业中使用植物有益菌作为有机改良剂来促进生长和保护病原体是一种有希望的替代目前过度使用化学品的方法。虽然这些产品中的许多已经上市，但它们的功效不一致是它们在田地和温室中广泛使用的主要障碍。来自芽孢杆菌属的细菌，特别是枯草杆菌和velezensis种的细菌，是农业生物解决方案发展的核心兴趣。这些细菌具有许多植物生长促进（PGP）的特性，如直接刺激植物的代谢，帮助植物获得不同的营养物质。大多数芽孢杆菌种类分泌抗菌武器库，有利于植物抵抗病原体感染，因此作为天然的生物农药和杀菌剂。芽孢杆菌种类也可以形成耐药的内生孢子，这是一种理想的休眠状态，适合产品配方。关于有益芽孢杆菌和植物之间相互作用的基本问题仍然没有答案，这减慢了我们制定一致有效产品的能力。在这个研究项目中，我们将探索三个基本问题，目的是更好地理解芽孢杆菌物种与植物之间复杂的相互作用。首先，我们将确定调节芽孢杆菌在根上休眠的环境条件。在同样的目的下，我们也将评估芽孢杆菌孢子是否仍然可以对植物产生有益的影响。其次，我们将确定哪些是芽孢杆菌细胞成分，这些成分对于在没有和存在竞争菌株的情况下在根上建立和持续存在至关重要。最后，我们将评估芽孢杆菌在植物根系上的有益活性表达程度，以及如何在自然条件下增强其活性。本研究将有助于进一步了解枯草芽孢杆菌与植物的相互作用，为该生物生长促进剂和农药的开发、利用和配方制定提供重要信息。这些知识非常重要，因为它们对于发展和植入可持续农业系统是必要的。