A Quantitative platform to expedite gene and genomic discovery from model species to crops and wild berries.

一个定量平台，可加快从模式物种到农作物和野生浆果的基因和基因组发现。

• 批准号: RTI-2023-00174
• 负责人: Song, Liang
• 金额: $ 7.92万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=746344
• 关键词: Quantitative; platform; expedite; gene; genomic

Quantitative and sensitive detection of changes in gene expression and mutations in genomes is essential for plant molecular biology studies. We request funding to establish a platform composed of a quantitative real-time PCR (qPCR) module and an integrated digital PCR (dPCR) module to be hosted in the Department of Botany at the University of British Columbia. The qPCR module is for an urgent replacement of a failed qPCR system that was frequently used over the past decade by approximately half of the research groups in the Department of Botany at UBC. The dPCR module will support the same user groups for quantitative assays that cannot be addressed by qPCR. The sensitivity, precision, inhibition resilience, and absolute quantification of dPCR allow the detection of rare transcripts, subtle but biologically crucial changes of gene expression, and naturally occurring or genome editing-induced mutations even in polyploid plant species or in the presence of PCR inhibitors. The quantitative platform will facilitate scientific discoveries and training of more than 50 HQP across the user groups in a broad range of research programs supported by at least eight NSERC Discovery grants. These research activities will benefit the many fundamental research programs in plant molecular biology, cell biology, biochemistry, metabolism, genomics, and immunity. The qPCR-dPCR platform will also generate quantitative data for crop improvement in species of high economic value to Canada, including oilseeds, wheat, blueberries, and tomatoes, as well as wild berries utilized by First Nations.

定量、灵敏地检测基因表达的变化和基因组突变对于植物分子生物学研究至关重要。我们请求资金建立一个由定量实时 PCR (qPCR) 模块和集成数字 PCR (dPCR) 模块组成的平台，该平台由不列颠哥伦比亚大学植物学系托管。 qPCR 模块用于紧急更换失败的 qPCR 系统，该系统在过去十年中被 UBC 植物学系约一半的研究小组频繁使用。 dPCR 模块将支持相同的用户组进行 qPCR 无法解决的定量分析。 dPCR 的灵敏度、精确度、抑制弹性和绝对定量可以检测罕见的转录本、基因表达的微妙但生物学上至关重要的变化，以及自然发生的或基因组编辑诱导的突变，甚至在多倍体植物物种中或存在 PCR 抑制剂的情况下也是如此。该定量平台将促进用户群体中 50 多个 HQP 的科学发现和培训，这些研究项目由至少八项 NSERC Discovery 拨款支持。这些研究活动将有利于植物分子生物学、细胞生物学、生物化学、新陈代谢、基因组学和免疫领域的许多基础研究项目。 qPCR-dPCR 平台还将生成对加拿大具有高经济价值的物种作物改良的定量数据，包括油籽、小麦、蓝莓和西红柿，以及原住民使用的野生浆果。