Development of pH-responsive carriers for the controlled release of encapsulated acidifying and antibacterial agents

随着不断出现的耐药微生物和新出现的传染病的幽灵日益逼近，在越来越多的行业中，建立和保持严格和最新的消毒措施已经变得至关重要。除了有效地中和病原体外，新型消毒剂配方必须尽可能地对环境无害，并且对处理这些物质的个人无毒。在卫生领域，氯作为一种直接的广谱消毒剂早已得到应用。然而，某些微生物，包括隐孢子虫——娱乐用水疾病中最普遍的病原体——对氯处理有抵抗力。要解决这一问题，要么需要开发新型消毒剂，要么需要改进现有方法的效率。为此，我们建议开发基于ph敏感脂质体的消毒剂配方。脂质体（脂质双层囊泡）是一种可持续的、固有的生物相容性结构，在递送胶囊化化合物方面有着广泛的应用，包括显著提高向细菌递送抗菌剂的效率。我们的系统将包括脂质体封装季铵和过氧乙酸消毒剂，随着ph值的变化，它们将经历不稳定并与微生物细胞融合。由于能够应用于散装溶液或作为表面处理，这种新型消毒剂输送系统将代表着卫生行业消毒剂实践与当代挑战相一致的重要一步。

With the ever-present specter of drug-resistant microbes and emerging infectious diseases looming large,establishing and maintaining stringent and up-to-date disinfection practices has taken on paramount importancein a growing number of industries. In addition to effectively neutralizing pathogens, novel disinfectantformulations must be, as much as possible, environmentally benign and nontoxic to individuals handling thesesubstances. In the sanitation field, chlorine has long found application as a straightforward broad-spectrumdisinfectant. However, certain microbes including Cryptosporidium - the most prevalent causative agent inrecreational water illnesses - are resistant to chlorine treatment. Addressing this issue requires either thedevelopment of novel disinfectants or refinement of the efficiency of existing approaches. To this end, wepropose to develop disinfectant formulations based on pH-sensitive liposomes. Liposomes (lipid bilayervesicles) are sustainable, inherently biocompatible structures that have found broad application in the deliveryof encapsulated compounds, including significantly increased efficiency in the delivery of antimicrobials tobacteria. Our system will comprise liposomes encapsulating quaternary ammonium and peracetic aciddisinfectants that will undergo destabilization and fusion with microbial cells following a change in pH. Withthe ability to be applied in the bulk solution or as a surface treatment, this novel disinfectant delivery systemwill represent a significant step forward in aligning disinfectant practices in the sanitation industry withcontemporary challenges.