Mapping heterotrophic functions to microbial population structures in wastewater resource recovery processes for optimization and prediction of antimicrobial resistance dissemination.

将异养功能映射到废水资源回收过程中的微生物种群结构，以优化和预测抗菌药物耐药性传播。

• 批准号: RGPIN-2022-04203
• 负责人: Frigon, Dominic
• 金额: $ 1.89万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=749500
• 关键词: Mapping; heterotrophic; functions; microbial; population

Microbial communities in sewer systems and in the Wastewater Resource Recovery Facilities (WRRFs) are connected through immigration driven by the influent wastewater. How sewer immigration shapes the microbial communities in WRRFs is still debated, but it is likely modulated by the specific bioprocess (e.g., aerobic vs. anaerobic) and the metabolic functions of the populations of interest. These considerations are important for the development of new bioprocesses that promote resource recovery, which increasingly utilize anaerobic technologies. They are also significant for the prediction of the dissemination of antimicrobial resistance genes (ARGs) through WRRFs. Municipal wastewater is recognized as a hotspot of ARGs, and the current antimicrobial resistance crisis demands to revise the operation of wastewater facilities that are not designed to reduce the dissemination of ARGs. These issues are at central to my research program with the long-term objective of developing novel wastewater resource recovery bioprocesses based on an understanding of their microbial ecology and to provide the theoretical framework for the rational design and optimization of the urban wastewater systems as a whole. In the short-term, this proposal aims to look in more details at the immigration interface between sewer and WRRF bioprocesses. Several tools will be developed and adapted to reach the following short-term goals including: (1) the development of new synthetic wastewater to manipulate the bioprocesses microbial community independently of immigration, (2) the use new flow cytometry-based techniques to reveal the metabolic functions heterotrophs across the immigration interface, (3) the use of a multiplex ARG amplicon sequencing tools to trace fate of specific allelic sequence variants across the interface. The results will provide information to optimize bioprocesses in function of the microbial communities immigrating with the influent wastewater, and to predict the fate of emerging pollutants like ARGs. Impact This work will train 2 PhDs, 2 Master's and 5 undergraduate students. Their training in both wastewater engineering and microbial community analyses/metagenomics will equip them well to enter an increasingly broad interdisciplinary workforce with opportunities in process development and public health. From a technical perspective, this work will provide the platform to evaluate a number of questions with respect to the immigration interface between sewers and WRRFs. The WRRF industry is not only charged with the development of more efficient bioprocesses, but also has to content with an increasing awareness of WRRFs as hotspots for the dissemination and recombination of ARGs. Microbial communities in sewers and WRRFs are critical for both these aspects. In this context, the framework of this proposal will inform the mechanism at play during immigration, and allow a wider prediction of microbial community responses to changing WRRF procedures.

下水道系统和废水资源化设施中的微生物群落通过流入废水驱动的移民而连接。下水道移民如何塑造WRRF中的微生物群落仍有争议，但它可能受到特定生物过程的调节（例如，需氧与厌氧）和目标群体的代谢功能。这些考虑因素对于开发促进资源回收的新生物工艺非常重要，这些工艺越来越多地利用厌氧技术。它们也是重要的抗菌素耐药基因（ARG）通过WRRF传播的预测。城市污水被认为是ARG的热点，当前的抗菌素耐药性危机要求修改废水设施的操作，这些设施的设计不是为了减少ARG的传播。这些问题是我的研究计划的核心，其长期目标是基于对其微生物生态学的理解开发新型废水资源回收生物工艺，并为城市污水系统的合理设计和优化提供理论框架。在短期内，该提案旨在更详细地研究下水道和WRRF生物过程之间的移民界面。将开发和调整若干工具，以实现以下短期目标，包括：（1）开发新的合成废水以独立于迁移来操纵生物过程微生物群落，（2）使用新的基于流式细胞术的技术来揭示跨迁移界面的异养生物的代谢功能，（3）使用多重ARG扩增子测序工具追踪特定等位基因序列变体在界面上的命运。结果将提供信息，以优化生物过程中的功能的微生物群落移民与进水废水，并预测新兴的污染物，如ARGs的命运。影响这项工作将培养2名博士，2名硕士和5名本科生。他们在废水工程和微生物群落分析/宏基因组学方面的培训将使他们能够很好地进入越来越广泛的跨学科劳动力队伍，并在工艺开发和公共卫生方面有机会。从技术角度来看，这项工作将提供一个平台来评估有关下水道和WRRF之间移民界面的许多问题。WRRF行业不仅负责开发更有效的生物工艺，而且还必须满足于日益认识到WRRF是ARG传播和重组的热点。下水道和WRRF中的微生物群落对这两个方面都至关重要。在这种情况下，该提案的框架将为移民期间发挥作用的机制提供信息，并允许更广泛地预测微生物群落对改变WRRF程序的反应。