Preventing mastitis in canadian dairy cattle while reducing industry reliance on antibiotics

预防加拿大奶牛乳腺炎，同时减少行业对抗生素的依赖

• 批准号: 575174-2022
• 负责人: Ronholm, JenniferJ
• 金额: $ 1.46万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=753526
• 关键词: Preventing; mastitis; canadian; dairy; cattle

Bovine mastitis is a bacterial infection that is common in dairy cattle. This disease is painful for cows, costs $665 million in losses annually for the Canadian dairy industry, and results in the use of large quantities of antibiotics. Antibiotic use in agriculture is becoming controversial and unsustainable due to the rising numbers of antibiotic resistant human infections; however, outright prohibition of antibiotic use in dairy farming would cause catastrophic and unsustainable losses due to the increased prevalence of bovine mastitis. In this project, we will partner with with two industrial partners to develop a topical probiotic solution that can be applied to the bovine teat to introduce a healthy microbiome and reduce the potential of pathogen colonization. This project will fill important knowledge gaps including the optimal composition of a healthy teat microbiome, how pathogens and symbionts interact in the teat environment, if teat symbionts can inhibit pathogen colonization, and if we can reliably manipulate the bovine teat microbiome. We will accomplish this by conducting culture based work that builds on and complements culture-independent work already completed in my laboratory - which has identified possible pathogen antagonists present in the microbiome of particularly healthy, and mastitis resistant, dairy cattle. We will ensure that this product is both effective at preventing mastitis by optimizing it with DeLaval, and that it meets the needs of dairy farmers by communicating our findings with them and receiving feed back via Dairy Farmers of Ontario. At the end of this project we aim to have a developed probiotic teat dip that is ready for clinical efficacy testing for preventing bovine mastitis.

牛乳腺炎是一种常见于奶牛的细菌感染。这种疾病对奶牛来说是痛苦的，每年给加拿大乳制品行业造成6.65亿美元的损失，并导致大量抗生素的使用。抗生素在农业中的使用正变得有争议和不可持续，因为抗生素耐药性的人类感染数量不断增加；然而，完全禁止在奶牛养殖中使用抗生素将造成灾难性的和不可持续的损失，因为牛乳腺炎的患病率增加。在这个项目中，我们将与两个工业伙伴合作开发一种局部益生菌解决方案，可以应用于牛乳头，引入健康的微生物群，减少病原体定植的可能性。该项目将填补重要的知识空白，包括健康奶牛微生物组的最佳组成，病原体和共生体如何在奶牛环境中相互作用，奶牛共生体是否可以抑制病原体定植，以及我们是否可以可靠地操纵牛奶牛微生物组。我们将通过开展基于培养的工作来实现这一目标，这项工作建立在我的实验室已经完成的与培养无关的工作的基础上，并对其进行补充。我的实验室已经确定了在特别健康和抗乳腺炎的奶牛的微生物组中可能存在的病原体拮抗剂。我们将通过与DeLaval进行优化，确保该产品既能有效预防乳腺炎，又能满足奶农的需求，通过与他们沟通我们的发现，并通过安大略省奶农协会获得反馈。在这个项目结束时，我们的目标是开发出一种益生菌乳浸，准备进行预防牛乳腺炎的临床疗效测试。