Whole-Genome Analysis of Klebsiella pneumoniae Isolated from instances of Bovine Mastitis in Canada

肺炎克雷伯氏菌是一种细菌病原体，可在人和奶牛中引起严重感染。由于抗菌素耐药性菌株的增加，它在人和牛身上变得越来越无法治疗。引起人类感染的肺炎克雷伯菌菌株与导致奶牛感染的菌株之间的关系知之甚少。目前尚不清楚人和牛之间是否存在显著的往返传播，也不清楚肺炎克雷伯菌是否完全不同的谱系导致了人和牛的感染。这项研究将对来自加拿大和爱尔兰的各种牛和人类分离株进行全基因组测序，并确定在这两个国家导致人类和奶牛患病的分离株的亲缘关系。这项研究产生的知识将有助于制定管理做法以及非抗生素疗法和预防措施，以预防、治疗和预防奶牛肺炎克雷伯菌感染。它还将被用来提供有用的公共政策和生物安全措施，旨在减少这一重要病原体的人畜共患病和反向人畜共患传播。

Klebsiella pneumoniae is a bacterial pathogen that can cause serious infections in both humans and dairy cattle. It is becoming increasingly untreatable in both humans and cattle due to the rise in antimicrobial resistant strains. Very little is known about the relationships between the K. pneumoniae strains that cause human infections and the strains that cause infections in dairy cows. It is unclear if there is significant transmission back and fourth between humans and cows, or if completely distinct lineages of K. pneumoniae are responsible for infections in humans and cows. This study will whole genome sequence a variety of bovine and human isolates from both Canada and Ireland and determine how related the isolates causing illness in humans and cows are in both countries. The knowledge generated by this study will help to develop management practices as well as non-antibiotic therapeutics and prophylactics to prevent treat and prevent K. pneumoniae infections in dairy cattle. It will also be usedto inform useful public policy and biosecurity measures aimed at reducing zoonotic and reverse-zoonotic transfer of this important pathogen.