Whole-Genome Analysis of Klebsiella pneumoniae Isolated from instances of Bovine Mastitis in Canada

肺炎克雷伯菌是一种细菌性病原体，可在人类和奶牛中引起严重感染。由于抗微生物菌株的增加，它在人类和牛身上变得越来越无法治疗。引起人类感染的肺炎克雷伯菌菌株与引起奶牛感染的菌株之间的关系所知甚少。目前尚不清楚人类和奶牛之间是否存在显著的传播，或者是否完全不同的肺炎克雷伯菌谱系导致人类和奶牛的感染。这项研究将对来自加拿大和爱尔兰的多种牛和人类分离株进行全基因组测序，并确定在这两个国家引起人类和奶牛疾病的分离株之间的相关性。这项研究产生的知识将有助于制定管理实践以及非抗生素治疗和预防措施，以预防和治疗奶牛中的肺炎克雷伯菌感染。它还将用于为有效的公共政策和生物安全措施提供信息，旨在减少这一重要病原体的人畜共患和反人畜共患转移。

Klebsiella pneumoniae is a bacterial pathogen that can cause serious infections in both humans and dairy cattle. It is becoming increasingly untreatable in both humans and cattle due to the rise in antimicrobial resistant strains. Very little is known about the relationships between the K. pneumoniae strains that cause human infections and the strains that cause infections in dairy cows. It is unclear if there is significant transmission back and fourth between humans and cows, or if completely distinct lineages of K. pneumoniae are responsible for infections in humans and cows. This study will whole genome sequence a variety of bovine and human isolates from both Canada and Ireland and determine how related the isolates causing illness in humans and cows are in both countries. The knowledge generated by this study will help to develop management practices as well as non-antibiotic therapeutics and prophylactics to prevent treat and prevent K. pneumoniae infections in dairy cattle. It will also be usedto inform useful public policy and biosecurity measures aimed at reducing zoonotic and reverse-zoonotic transfer of this important pathogen.