Study on the viscoelastic properties of gels from the extra-cellular matrix of decellularized tissue for therapeutic applications in cardiac and parenchymal tissue

研究脱细胞组织细胞外基质凝胶的粘弹性特性，用于心脏和实质组织的治疗应用

• 批准号: 578501-2022
• 负责人: Mantovani, DiegoD
• 金额: $ 3.64万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=753943
• 关键词: Study; viscoelastic; properties; gels; extra

The field of health, including surgery and regenerative medicine, requires materials allowing the migration, retention and growth of healthy cells from the surrounding tissues of the patient. In the form of gels and hydrogels, these materials are considered scaffold structures. They can be injected directly (without surgery) into the pathological site requiring treatment, or molded or printed, and then implanted using conventional surgery. In any case, the elastic and viscous properties of these highly hydrated materials are essential in order to modulate the gelation and the biological properties. Currently, the ability to cellularize these materials, by integrating cells, is the next frontier in this field. The characterization of cellularized samples would make it possible to develop gels which, when implanted, would be able to regenerate and replace the functions of diseased tissues. In the proposed project, the first objective consists in the development of a novel sample holder for the contactless viscoelastic characterization of cellularized hydrogels to be used with the innovative and unique theological equipment developed by Rheolution. Second, thanks to the presence of Tissuegraft, and their expertise in formulating hydrogels from decellularized extracellular matrix extracted from animal tissues, will be the optimization of the composition of a gel stimulating recruitment and regeneration from human cells for the repair and regeneration of defects in cardiac or parenchymal organs, using the sample holder developed by Rheolution and validated within the framework of the 1st objective. The unique expertise at the Biomaterials and Bioengineering Laboratory of ULaval, combining the modification of surface properties and the mechanical characterization of the gels developed, constitutes the keystone to allow the characterization of these cellularized gels. Finally, the third objective is to explore the potential for the development of a new in vitro model based on the developed Rheolution sample holder and Tissuegraft hydrogels.

包括外科手术和再生医学在内的健康领域需要允许健康细胞从患者周围组织迁移、保留和生长的材料。以凝胶和水凝胶的形式，这些材料被认为是支架结构。它们可以直接注射（无需手术）到需要治疗的病理部位，或模制或打印，然后使用常规手术植入。在任何情况下，这些高度水合的材料的弹性和粘性对于调节凝胶化和生物学性质是必不可少的。目前，通过整合细胞使这些材料细胞化的能力是该领域的下一个前沿。细胞化样品的表征将使开发凝胶成为可能，当植入时，凝胶将能够再生并取代患病组织的功能。在拟议的项目中，第一个目标是开发一种新型样品保持器，用于细胞化水凝胶的非接触粘弹性表征，并与Rheolution开发的创新和独特的流变设备一起使用。其次，由于Tissuegraft的存在，以及他们在从动物组织中提取的脱细胞细胞外基质配制水凝胶方面的专业知识，将优化凝胶的组成，刺激人体细胞的募集和再生，用于心脏或实质器官缺陷的修复和再生，使用Rheolution开发的样品保持器，并在第一个目标的框架内进行验证。ULaval生物材料和生物工程实验室的独特专业知识，结合了表面性质的改性和所开发凝胶的机械表征，构成了对这些细胞化凝胶进行表征的基石。最后，第三个目标是探索基于开发的Rheolution样品保持器和Tissuegraft水凝胶开发新体外模型的潜力。