The Case of the Missing Macrophages: Investigating the role of NKR-P1B:Clr-b self-recognition on tissue-resident myeloid cells.

巨噬细胞缺失的案例:研究 NKR-P1B:Clr-b 自我识别对组织驻留骨髓细胞的作用。

基本信息

  • 批准号:
    RGPIN-2018-05557
  • 负责人:
  • 金额:
    $ 5.25万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2022
  • 资助国家:
    加拿大
  • 起止时间:
    2022-01-01 至 2023-12-31
  • 项目状态:
    已结题

项目摘要

Innate immune cell functions are regulated through sophisticated systems of pattern-recognition receptors. One example of a receptor system that regulates innate immune responses is the NKR-P1 family of C-type lectin-like receptors, and its binding ligands, the C-type lectin-related (Clr) family of surface proteins. NKR-P1B is an inhibitory member of this receptor family, which recognizes Clr-b, and is expressed on natural killer cells. Using genetically modified mice lacking NKR-P1B expression, we have observed a marked impairment in lung alveolar macrophage (AM) numbers, a completely unexpected phenotype. We have also found the NKR-P1B is expressed on AMs. We now seek to understand how NKR-P1B:Clr-b recognition system regulates lung-resident myeloid immune cell development and function. We have proposed the follow two objectives:1. Discover the impact of NKR-P1B expression on AMs and DCs. We will fully characterize the functional and phenotypic differences between wild-type and NKR-P1B-deficient lung-resident AM and dendritic cells (DC). Using confocal microscopy, we will analyze lungs of mice at various ages to determine AM distribution and phenotype. We will perform several functional assays, including cytokine release, phagocytosis, and antigen-presenting assays in the presence of NKR-P1B cross-linking antibodies to simulate the effects of NKR-P1B engagement on normal myeloid cell function. Finally, using bone-marrow chimeras to study NKR-P1B-deficient myeloid cells in wild-type environments and vice versa, we will investigate whether NKR-P1B effects on the myeloid cell populations are cell-intrinsic or extrinsic.2. Discover the mechanism through which NKR-P1B exerts its effects on AMs and DCs. We have observed evidence for impaired metabolic processing in NKR-P1B-deficient AMs. We will compare the metabolic profiles of WT and NKR-P1B-deficient AMs, as well as their transcriptomes obtained by RNA sequence (RNAseq) analysis to determine differentially expressed genes, and the cellular and metabolic pathways that are involved. We will also investigate NKR-P1B interacting and cell signaling partners in macrophages using the proximity-biotinylation BioID system.Understanding myeloid immune cell development and function strengthens our understanding of cells in general. We have fortuitously stumbled into a previously unappreciatedand apparently criticalcomponent of alveolar macrophage biology, and it is our hope that exploring the involvementboth overall role and specific signaling pathwaysof NKR-P1B:Clr-b interactions in these cells will teach us fundamental lessons about myeloid cell development and function.
先天免疫细胞的功能通过复杂的模式识别受体系统进行调节。调节先天免疫应答的受体系统的一个实例是C型凝集素样受体的NKR-P1家族及其结合配体,表面蛋白的C型凝集素相关(Clr)家族。NKR-P1B是该受体家族的抑制性成员,其识别Clr-b,并在自然杀伤细胞上表达。使用缺乏NKR-P1B表达的转基因小鼠,我们观察到肺泡巨噬细胞(AM)数量明显受损,这是一种完全出乎意料的表型。我们还发现NKR-P1B在AM上表达。我们现在试图了解NKR-P1B:Clr-b识别系统如何调节肺驻留骨髓免疫细胞的发育和功能。我们提出了以下两个目标:1。发现NKR-P1B表达对AM和DC的影响。我们将充分表征野生型和NKR-P1B缺陷型肺驻留AM和树突状细胞(DC)之间的功能和表型差异。利用共聚焦显微镜,我们将分析在不同年龄的小鼠肺,以确定AM分布和表型。我们将在NKR-P1B交联抗体存在的情况下进行几项功能测定,包括细胞因子释放、吞噬作用和抗原呈递测定,以模拟NKR-P1B接合对正常骨髓细胞功能的影响。最后,使用骨髓嵌合体研究野生型环境中NKR-P1B缺陷型髓系细胞,反之亦然,我们将研究NKR-P1B对髓系细胞群体的影响是细胞内在的还是细胞外的.发现NKR-P1B对AM和DC发挥作用的机制。我们已经观察到NKR-P1B缺陷型AM代谢过程受损的证据。我们将比较WT和NKR-P1B缺陷型AM的代谢谱,以及通过RNA序列(RNAseq)分析获得的转录组,以确定差异表达的基因,以及所涉及的细胞和代谢途径。我们还将使用邻近生物素化BioID系统研究巨噬细胞中的NKR-P1B相互作用和细胞信号传导伙伴。了解骨髓免疫细胞的发育和功能增强了我们对细胞的理解。我们偶然发现了肺泡巨噬细胞生物学中一个以前未被重视的、显然至关重要的组成部分,我们希望探索这些细胞中NKR-P1B:Clr-b相互作用的整体作用和特异性信号通路,将为我们提供有关骨髓细胞发育和功能的基本经验。

项目成果

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Makrigiannis, Andrew其他文献

Makrigiannis, Andrew的其他文献

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{{ truncateString('Makrigiannis, Andrew', 18)}}的其他基金

The Case of the Missing Macrophages: Investigating the role of NKR-P1B:Clr-b self-recognition on tissue-resident myeloid cells.
巨噬细胞缺失的案例:研究 NKR-P1B:Clr-b 自我识别对组织驻留骨髓细胞的作用。
  • 批准号:
    RGPIN-2018-05557
  • 财政年份:
    2021
  • 资助金额:
    $ 5.25万
  • 项目类别:
    Discovery Grants Program - Individual
The Case of the Missing Macrophages: Investigating the role of NKR-P1B:Clr-b self-recognition on tissue-resident myeloid cells.
巨噬细胞缺失的案例:研究 NKR-P1B:Clr-b 自我识别对组织驻留骨髓细胞的作用。
  • 批准号:
    RGPIN-2018-05557
  • 财政年份:
    2020
  • 资助金额:
    $ 5.25万
  • 项目类别:
    Discovery Grants Program - Individual
The Case of the Missing Macrophages: Investigating the role of NKR-P1B:Clr-b self-recognition on tissue-resident myeloid cells.
巨噬细胞缺失的案例:研究 NKR-P1B:Clr-b 自我识别对组织驻留骨髓细胞的作用。
  • 批准号:
    RGPIN-2018-05557
  • 财政年份:
    2019
  • 资助金额:
    $ 5.25万
  • 项目类别:
    Discovery Grants Program - Individual
The Case of the Missing Macrophages: Investigating the role of NKR-P1B:Clr-b self-recognition on tissue-resident myeloid cells.
巨噬细胞缺失的案例:研究 NKR-P1B:Clr-b 自我识别对组织驻留骨髓细胞的作用。
  • 批准号:
    RGPIN-2018-05557
  • 财政年份:
    2018
  • 资助金额:
    $ 5.25万
  • 项目类别:
    Discovery Grants Program - Individual
Deletion of repetitive gene families
删除重复基因家族
  • 批准号:
    386878-2010
  • 财政年份:
    2010
  • 资助金额:
    $ 5.25万
  • 项目类别:
    Discovery Grants Program - Individual
PGSB/ESB
PGSB/ESB
  • 批准号:
    189840-1996
  • 财政年份:
    1998
  • 资助金额:
    $ 5.25万
  • 项目类别:
    Postgraduate Scholarships

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Missing in Metastasis基因在子宫内膜癌转移中的机制
  • 批准号:
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