LC-MS for radiochemistry and chemistry characterization

用于放射化学和化学表征的 LC-MS

• 批准号: RTI-2023-00195
• 负责人: Yang, Hua
• 金额: $ 10.48万
• 依托单位: TRIUMF
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=761536
• 关键词: LC; MS; radiochemistry; chemistry; characterization

This proposal is to acquire a liquid chromatography - mass spectrometer (LC-MS) for analysis of radioactive and nonradioactive samples at TRIUMF. Currently, there is no MS or Nuclear Magnetic Resonance (NMR) instrument for direct chemical characterization at TRIUMF. MS is an essential technique for compound identification and reaction monitoring. For radioactive compounds, the conventional practice for their identification is still solely based on chromatographic (radio-TLC and HPLC) analysis, despite modern chemistry long requiring multiple direct characterizations. The reliance on chromatographic information becomes more precarious when working with radionuclides with low gamma emissions that are hard to detect. Furthermore, tracking the chromatograms of radioactive products is complicated if multiple daughter ions are present in the decay chain, such as with 225Ac or 227Th. Hence, there is a rising trend to obtain more thorough characterization of radioactive compounds. To keep pace with the increasing scientific standards, TRIUMF would hugely benefit from having an LC-MS, giving us a much-needed tool to identify or confirm the composition of our compounds. The LC-MS instrument would be frequently used by the researchers at TRIUMF. In addition to radioactive compounds, its use would involve nonradioactive samples, enabling us to fast-track the synthesis of new chelators and bioconjugates. Having an LC-MS instrument on-site, the productivity of our students involved in synthetic projects would be greatly enhanced, helping them with their thesis projects. Our HQPs would also benefit by having a highly marketable skill with hands-on LC-MS experience. Several NSERC funded programs will be supported, including four DG programs centered on chelator and bioconjugate development for unconventional radionuclides. There is no LC-MS instrument setup to run radioactive compounds by our university partners and there is no other financially feasible alternative. Thus, our LC-MS instrument will be shared among researchers within TRIUMF and from 21 member universities and other collaborators, providing a unique platform for spectrometric analysis of radioactive compounds and helping us understand the fundamental chemistry of some radionuclides that hard to characterize by conventional methods. The instrument required is either a mass detector from Agilent that can be connected to our existing LC, or a stand-alone LC-MS system from Waters. The Agilent system is preferred due to higher sensitivity and larger mass range. TRIUMF has extensive experience setting up instruments that need nuclear ventilation. A dedicated instrument technician will maintain the instrument and provide hands-on training. In short, the equipment will raise the standard for radioactive compound characterization, generate more scientifically sound results, deepen our knowledge of radioactive compounds, support several NSERC funded programs, and deliver well-rounded HQP training.

该提案旨在获得一台液相色谱-质谱仪（LC-MS），用于分析TRIUMF的放射性和非放射性样品。目前，TRIUMF没有MS或核磁共振（NMR）仪器用于直接化学表征。MS是化合物鉴定和反应监测的基本技术。对于放射性化合物，其鉴定的常规实践仍然仅基于色谱（放射性TLC和HPLC）分析，尽管现代化学长期以来需要多个直接表征。当使用难以检测的低伽马放射性核素时，对色谱信息的依赖变得更加不稳定。此外，如果衰变链中存在多个子离子，如225 Ac或227 Th，则跟踪放射性产物的色谱图是复杂的。因此，有一个上升的趋势，以获得更彻底的表征放射性化合物。为了跟上不断提高的科学标准，TRIUMF将从LC-MS中受益匪浅，为我们提供了一个急需的工具来识别或确认我们化合物的组成。LC-MS仪器将经常被TRIUMF的研究人员使用。除了放射性化合物，它的使用将涉及非放射性样品，使我们能够快速跟踪新螯合剂和生物缀合物的合成。有一个LC-MS仪器现场，我们的学生参与合成项目的生产力将大大提高，帮助他们与他们的论文项目。我们的HQP也将受益于具有高度市场化的技能和实践LC-MS经验。几个NSERC资助的项目将得到支持，包括四个集中在螯合剂和非常规放射性核素生物共轭物开发的DG项目。我们的大学合作伙伴没有LC-MS仪器设置来运行放射性化合物，也没有其他经济上可行的替代方案。因此，我们的LC-MS仪器将在TRIUMF内部的研究人员以及来自21所成员大学和其他合作者之间共享，为放射性化合物的光谱分析提供一个独特的平台，并帮助我们了解一些难以用常规方法表征的放射性核素的基本化学性质。所需的仪器可以是Agilent的质量检测器，可以连接到我们现有的LC，或者是沃茨的独立LC-MS系统。Agilent系统是首选，因为它具有更高的灵敏度和更大的质量范围。TRIUMF在设置需要核通风的仪器方面拥有丰富的经验。专门的仪器技术人员将维护仪器并提供实践培训。简而言之，该设备将提高放射性化合物表征的标准，产生更科学合理的结果，加深我们对放射性化合物的了解，支持几个NSERC资助的项目，并提供全面的HQP培训。