Mass spectrometer (LC-MS)

质谱仪（LC-MS）

• 批准号: 537496341
• 金额: --
• 依托单位: Friedrich-Alexander-Universität Erlangen-Nürnberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2024
• 资助国家: 德国
• 起止时间: 2023-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/537496341?language=en
• 关键词: Mass; spectrometer; LC; MS

This grant application is for a highly sensitive mass spectrometer including a high-performance liquid chromatography system and associated software for the analytics of drugs and endogenous metabolites. Besides the quantification of known drugs, endogenous metabolites and peptides, this system will also support the qualitative identification of structurally so far unknown molecules. The mass spectrometry facility at the Chair of Clinical Pharmacology and Clinical Toxicology is developing and applying quantitative methods for a wide variety of small endogenous metabolites and drugs in different matrices in own research projects as well as in co-operations. The requested system is supposed to replace an old mass spectrometer, that went out of service, and therefore new analytical capacities should be generated. The focus lies on a liquid chromatography mass spectrometer system with high analytical sensitivity in order to achieve low limits of detection and quantification, which are well below those of mass spectrometers currently available at the mass spectrometry facility. Besides the afore mentioned highly sensitive quantification, the new mass spectrometer will expand the present possibilities in the context of untargeted metabolomics. One of the biggest challenges lies in the correct identification of detected signals. The requested mass spectrometer can analyze fragmentation patterns with an additional layer of fragmentation, which constitutes as an important aid for structure elucidation, and hence can help to increase the validity of untargeted metabolomics analyses. Furthermore, it is planned to use commercially available kits for targeted metabolomics with the requested system to complement untargeted metabolomics analyses.

该拨款申请适用于高灵敏度质谱仪，包括高性能液相色谱系统和用于分析药物和内源代谢物的相关软件。除了对已知药物、内源代谢物和肽进行定量之外，该系统还将支持迄今为止结构未知的分子的定性鉴定。临床药理学和临床毒理学主席的质谱设施正在自己的研究项目以及合作中开发和应用不同基质中各种小内源代谢物和药物的定量方法。所请求的系统应该取代已经停止使用的旧质谱仪，因此应该产生新的分析能力。重点在于具有高分析灵敏度的液相色谱质谱仪系统，以实现低检测和定量限，远低于目前质谱设施中可用的质谱仪。除了上述高灵敏度定量之外，新型质谱仪还将扩展非靶向代谢组学背景下的现有可能性。最大的挑战之一在于正确识别检测到的信号。所要求的质谱仪可以分析带有附加碎片层的碎片模式，这对结构阐明来说是一个重要的帮助，因此可以帮助提高非靶向代谢组学分析的有效性。此外，计划使用市售的靶向代谢组学试剂盒和所需的系统来补充非靶向代谢组学分析。