Particle Analyzer for Nanotechnology Applications

用于纳米技术应用的颗粒分析仪

• 批准号: RTI-2023-00554
• 负责人: Badea, Ildiko
• 金额: $ 10.93万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=762122
• 关键词: Particle; Analyzer; Nanotechnology; Applications

The pandemic heightened the need to urgently develop new nanotechnology-based vaccines and tests. For this, new nanomaterials are required. The cornerstone of research in this area is the elucidation of physicochemical properties of nanosystems, specifically size and surface charge measurements, using a zetasizer. We require a new zetasizer to replace an aging instrument that is heavily utilized and is the only instrument of this kind at the University of Saskatchewan. Size and surface charge measurement are based on light scattering, the only technology that provides quantitative high-resolution data. Alternative methods for assessing the size and morphology of nanostructures, such as high-resolution electron or atomic-force microscopy and fluorescent microscopy do not provide quantitative data; require expensive instrumentation; are low throughput; and require processing of samples that might alter their native behaviour. Moreover, none of these alternative techniques is suitable to measure surface charge. Our team of six investigators conducts research in the areas of development of new biomaterials, engineering nanocarriers for nucleic acids and proteins, development of vaccines, and improvement of function of medical devices. All these areas rely heavily on the use of a zetasizer to optimize composition and properties of nanostructures for a variety of applications and establish structure-function/activity relationships. We belong to three research communities: Drug Discovery and Development Research Group, Vaccine and Infectious Disease Organization, and Biomedical Engineering. Our work with the instrument has resulted in numerous conference presentations and invited talks and more than 200 publications in high-impact journals. The zetasizer has been instrumental in catalyzing collaborations among our team members and other researchers. It is also a common element enabling HQP to be exposed to and learn from each other's projects and start conversations. To date, our team has trained over 70 HQP who used the zetasizer in their research projects. The diversity of our areas is invaluable for training HQP who develop broad expertise in multidisciplinary fields, including pharmaceutical sciences, chemistry, veterinary sciences, vaccinology, and engineering. During their training, our HQP elucidate the basic properties of nanomaterials such as size and surface charge and gain marketable skills to engineer new delivery systems and vaccines, and design new medical devices.

这一流行病突出表明，迫切需要开发新的基于纳米技术的疫苗和检测方法。 为此，需要新的纳米材料。这一领域研究的基石是使用zetasizer阐明纳米系统的物理化学性质，特别是尺寸和表面电荷测量。 我们需要一个新的zetasizer，以取代老化的仪器，这是大量使用，是唯一的这种仪器在萨斯喀彻温大学。尺寸和表面电荷测量基于光散射，这是唯一提供定量高分辨率数据的技术。用于评估纳米结构的尺寸和形态的替代方法，例如高分辨率电子或原子力显微镜和荧光显微镜不提供定量数据;需要昂贵的仪器;低通量;并且需要处理可能改变其天然行为的样品。此外，这些替代技术都不适合测量表面电荷。我们的六名研究人员团队在新生物材料的开发，核酸和蛋白质的工程纳米载体，疫苗的开发和医疗设备功能的改进等领域进行研究。所有这些领域都严重依赖于使用zetasizer来优化各种应用的纳米结构的组成和性质，并建立结构-功能/活性关系。我们属于三个研究社区：药物发现和开发研究组，疫苗和传染病组织，以及生物医学工程。我们与该仪器的合作已经促成了许多会议演讲和特邀演讲，并在高影响力期刊上发表了200多篇论文。 zetasizer在促进我们的团队成员和其他研究人员之间的合作方面发挥了重要作用。这也是一个共同的因素，使HQP能够接触和学习彼此的项目，并开始对话。到目前为止，我们的团队已经培训了70多名在研究项目中使用zetasizer的HQP。我们领域的多样性对于培养HQP非常宝贵，他们在多学科领域发展广泛的专业知识，包括制药科学，化学，兽医科学，疫苗学和工程学。在培训期间，我们的HQP阐明了纳米材料的基本特性，如尺寸和表面电荷，并获得了设计新的输送系统和疫苗以及设计新的医疗器械的市场技能。