Cortico-limbic-striatal network in stimulus and temporal control over adaptive behaviour

皮质-边缘-纹状体网络在刺激和适应性行为的时间控制中

• 批准号: RGPIN-2022-04665
• 负责人: Ito, Rutsuko
• 金额: $ 3.42万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=764215
• 关键词: Cortico; limbic; striatal; network; stimulus

Adaptive behaviour requires the continuous appraisal and selection of motivationally significant information in the external and internal environment. For instance, an organism surviving in the wild relies on their ability to discern changing spatiotemporal signals associated with reward and punishment contingencies as well as internal states in order to make decisions about when, where and what to eat. Past work from our laboratory has identified areas of the limbic cortico-striatal system to be critical in mediating spatial and contextual cue control over motivated behaviour. The present proposal will continue our endeavour to delineate the brain network and circuit mechanisms that facilitate the deployment of appropriate behavioural response to biologically relevant cues that signal the presence and absence of reward or threat, as well as extend our investigations into the temporal control over motivated behaviour. The proposed program will employ a multidisciplinary approach, using in vivo optogenetic, chemogenetic, fibre photometry and immunohistochemical methods in combination with customised rat behavioural paradigms, to achieve the following objectives: 1) To establish the key players in the cortico-limbic-striatal network that are recruited by discrete cues and context that signal the availability of reward, threat, absence of reward, and safety from threat, as well as cues signalling temporal information. 2) To investigate the causal role of the hub areas and circuits identified in the first objective, in cue and temporal control over motivated behaviour. 3) To investigate the dynamic interactions between connected hub areas in cue control over motivated behaviour. This research will provide critical and fundamental insights into how the brain is organized in processing temporal and non-temporal information pertaining to reward, safety and aversion and the circuit mechanisms by which such information gain control over motivated behaviour.

适应性行为需要不断评估和选择外部和内部环境中对动机有重要意义的信息。例如，在野外生存的有机体依赖于它们辨别与奖励和惩罚偶然事件以及内部状态相关的变化的时空信号的能力，以便决定何时，何地和吃什么。我们实验室过去的工作已经确定了边缘皮质-纹状体系统在调节空间和背景线索控制动机行为方面的关键区域。目前的建议将继续我们的努力，描绘大脑网络和电路机制，促进部署适当的行为反应的生物相关的线索，信号的存在和不存在的奖励或威胁，以及扩展我们的调查到时间控制的动机行为。 拟议的计划将采用多学科方法，使用体内光遗传学，化学遗传学，纤维光度学和免疫组织化学方法结合定制的大鼠行为模式，以实现以下目标：1）建立皮质-边缘-纹状体网络中的关键参与者，这些参与者由离散的线索和背景招募，这些线索和背景表明奖励的可用性、威胁、奖励的缺乏以及免受威胁的安全性，以及用信号通知时间信息的线索。 2)探讨第一个目标中确定的中枢区域和回路在动机行为的线索和时间控制中的因果作用。3)研究动机行为线索控制中中枢连接区之间的动态相互作用。 这项研究将提供关键和基本的见解，大脑是如何组织在处理有关奖励，安全和厌恶的时间和非时间信息和电路机制，这些信息获得控制动机行为。