Redox signaling: A key element for the development of new post-harvest conditioning technologies

氧化还原信号：开发新的采后调理技术的关键要素

• 批准号: RGPIN-2022-03930
• 负责人: DuarteSierra, Arturo
• 金额: $ 1.89万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=764730
• 关键词: Redox; signaling; key; element; development

The intake of horticultural crops is of utmost importance due to the amount of nutrients they contain, as well as the generally accepted inverse relationship between their consumption and the decrease in degenerative diseases. Thus, the supply chain of these products must be secured in all its stages, the last one being post-harvest storage. Proper handling of fruits and vegetables results in economic benefits for the entire production chain, as well as customer satisfaction. However, this can be taken a step further, and through post-harvest treatments the primary and secondary metabolism of produce can be modified. These alterations can increase the shelf life of produce and lead to the development of functional fresh produce. The latter include fresh produce with an increased amount of phytochemical compounds, fresh produce with pharmacological (i.e., immunological) character, as well as fresh produce with improved taste and odor characteristics. Previously, we have shown that different abiotic stresses appear to be beneficial in maintaining the quality and storability of produce at low doses. However, we haven't discerned the early events that cause these positive changes. This raises the question of whether it is possible to develop new storage technologies for plant products by unveiling early events related to the calcium and electrical waves, as well as redox signaling in produce. In this context, this research will attempt to relate, redox regulated and phytohormone signaling to modifications of primary and secondary metabolism in horticultural products by oxidative and reductive stresses. The long-term objective of this research program is to develop tailored post-harvest treatments to achieve functional commodities, either with enhanced flavor and odor characteristics, improved phytochemical composition and longer storage life. The importance of this research is that added value can be given to the horticultural products produced in Canada, but also to those that the country imports. Therefore, this could lead to the start of a new sector in the fresh unprocessed industry. In the short and medium term, the framework of the research program related to fresh produce will include: (1) the influence of ethylene in the redox state and overall metabolism; (2) the effect of long-term storage on redox status and nutritional quality; 3) the modification of primary and secondary metabolism due to reducing and oxidative stresses; and 4) the role of phytohormones in the production of ROS derived from the priming action of novel volatile fungicides of bacterial origin. The studies will be carried out using tomatoes, haskaps, wild blueberries and strawberries. Thus, the development of a conceptual map is proposed to explain in a mechanistic way the roll of redox signaling in post-harvest to improve the quality of fresh produce.

园艺作物的摄入量是至关重要的，因为它们含有大量的营养素，以及它们的消费和退化性疾病的减少之间普遍接受的反比关系。因此，这些产品的供应链必须在所有阶段得到保障，最后一个阶段是收获后的储存。正确处理水果和蔬菜可为整个生产链带来经济效益，并提高客户满意度。然而，这可以更进一步，通过收获后处理，可以改变农产品的初级和次级代谢。这些改变可以延长产品的保质期，并导致功能性新鲜产品的开发。后者包括具有增加量的植物化学化合物的新鲜农产品，具有药理学（即，免疫学）特性，以及具有改善味道和气味特性的新鲜产品。此前，我们已经证明，不同的非生物胁迫似乎有利于在低剂量下保持农产品的质量和可储存性。然而，我们还没有发现导致这些积极变化的早期事件。这就提出了一个问题，即是否有可能通过揭示与钙和电波有关的早期事件以及农产品中的氧化还原信号来开发新的植物产品储存技术。在这种情况下，本研究将试图联系，氧化还原调节和植物激素信号的修改，在园艺产品的初级和次级代谢的氧化和还原应力。该研究计划的长期目标是开发量身定制的收获后处理，以实现功能性商品，无论是增强风味和气味特征，改善植物化学成分和更长的储存寿命。这项研究的重要性在于，可以为加拿大生产的园艺产品以及该国进口的园艺产品提供附加值。因此，这可能导致在新鲜未加工工业中开始一个新的部门。在短期和中期内，与新鲜农产品有关的研究计划框架将包括：（1）乙烯在氧化还原状态和总体代谢中的影响;（2）长期储存对氧化还原状态和营养品质的影响;（3）由于还原和氧化应激而引起的初级和次级代谢的改变;（4）乙烯在氧化还原状态和总体代谢中的作用。和4）植物激素在由细菌来源的新型挥发性杀真菌剂的引发作用产生ROS中的作用。这些研究将使用西红柿，haskaps，野生蓝莓和草莓进行。因此，提出了一个概念图的发展，以机械的方式来解释在收获后的氧化还原信号的滚动，以提高新鲜农产品的质量。