Metabolic programming of embryo epigenetic profile in bovine

牛胚胎表观遗传谱的代谢编程

• 批准号: 571282-2021
• 负责人: Sirard, MarcAndréMA
• 金额: $ 1.82万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=765078
• 关键词: Metabolic; programming; embryo; epigenetic; profile

This potential new collaboration is based on complementary interest and expertise between the laboratory of Dr Sirard in Canada and Dr Milazzotto in Brazil. The thematic of Dr Sirard's lab is the development of Assisted Reproduction Technologies (ART) in the bovine species using comprehensive physiological approaches and more recently genomic tools to better understand the oocyte/embryo quality and to apply this knowledge in animal biotechnologies. Dr Milazzotto has become a specialist of bovine embryo metabolism and has the biochemical expertise to dissect the complex metabolic pathways regulating embryo survival and development. She is also an expert in culture media for the bovine embryos and the application of new culture conditions. The new collaboration to be initiated will combine these 2 fields and will start addressing the very important question of how the metabolic status of the embryo can program the future metabolic health of the newborn? More precisely, we want to unravel the mechanism by which the embryo's metabolites, in response to the culture environment, direct the epigenetic programming process on histones and DNA (methylation) including the mitochondria DNA. Understanding such a fundamental question can lead to targeted improvement in culture conditions leading to better health in in vitro produced bovine embryos. To initiate this collaboration, the Brazilian laboratory will prepare embryo's samples in 3 different metabolic conditions to assess phenotypic and biochemical consequences and then one student from Milazzotto's lab will come to Canada to perform the epigenetic analysis for which Dr Sirard's lab has developed new approaches for micro samples as well as for mitochondrial DNA. These results will demonstrate the possibility and the value of using metabolic changes to modify the epigenome of early embryos in a very important domestic animal species.

这种潜在的新合作是基于加拿大Sirard博士和巴西Milazzotto博士实验室之间的互补兴趣和专业知识。Sirard博士实验室的主题是使用全面的生理方法和最近的基因组工具在牛物种中开发辅助生殖技术（ART），以更好地了解卵母细胞/胚胎质量，并将这些知识应用于动物生物技术。 Milazzotto博士已成为牛胚胎代谢的专家，并拥有生物化学专业知识来剖析调节胚胎存活和发育的复杂代谢途径。她还是牛胚胎培养基和新培养条件应用方面的专家。即将启动的新合作将联合收割机结合这两个领域，并将开始解决胚胎的代谢状态如何规划新生儿未来代谢健康的非常重要的问题。 更确切地说，我们想解开胚胎代谢物响应培养环境的机制，指导组蛋白和DNA（甲基化）包括线粒体DNA的表观遗传编程过程。 了解这样一个基本问题可以导致有针对性地改善培养条件，从而改善体外生产的牛胚胎的健康状况。 为了启动这项合作，巴西实验室将在3种不同的代谢条件下制备胚胎样本，以评估表型和生化结果，然后Milazzotto实验室的一名学生将前往加拿大进行表观遗传分析，Sirard博士的实验室已经开发了用于微量样本和线粒体DNA的新方法。这些结果将证明在一个非常重要的家畜物种中利用代谢变化来修饰早期胚胎表观基因组的可能性和价值。