Metabolic programming of embryo epigenetic profile in bovine

这一潜在的新合作是基于加拿大的Sirard博士和巴西的Milazzotto博士的实验室之间的互补兴趣和专业知识。Sirard博士实验室的主题是利用全面的生理学方法和最近的基因组工具在牛物种中开发辅助生殖技术(ART)，以更好地了解卵母细胞/胚胎的质量，并将这一知识应用于动物生物技术。米拉佐托博士已经成为牛胚胎新陈代谢的专家，他拥有分析调控胚胎存活和发育的复杂代谢途径的生化专业知识。她也是牛胚胎培养介质和新培养条件应用方面的专家。即将启动的新合作将结合这两个领域，并将开始解决非常重要的问题，即胚胎的代谢状态如何规划新生儿未来的代谢健康？更准确地说，我们想要揭开胚胎代谢物对培养环境的反应，引导组蛋白和DNA(甲基化)包括线粒体DNA的表观遗传编程过程的机制。了解这样一个基本问题可以导致有针对性地改善培养条件，从而在体外生产的牛胚胎中获得更好的健康。为了启动这项合作，巴西实验室将准备3种不同代谢条件下的胚胎样本，以评估表型和生化后果，然后米拉佐托实验室的一名学生将来到加拿大进行表观遗传学分析，Sirard博士的实验室为此开发了用于微量样本和线粒体DNA的新方法。这些结果将证明利用代谢变化来修改一种非常重要的家畜物种早期胚胎的表观基因组的可能性和价值。

This potential new collaboration is based on complementary interest and expertise between the laboratory of Dr Sirard in Canada and Dr Milazzotto in Brazil. The thematic of Dr Sirard's lab is the development of Assisted Reproduction Technologies (ART) in the bovine species using comprehensive physiological approaches and more recently genomic tools to better understand the oocyte/embryo quality and to apply this knowledge in animal biotechnologies. Dr Milazzotto has become a specialist of bovine embryo metabolism and has the biochemical expertise to dissect the complex metabolic pathways regulating embryo survival and development. She is also an expert in culture media for the bovine embryos and the application of new culture conditions. The new collaboration to be initiated will combine these 2 fields and will start addressing the very important question of how the metabolic status of the embryo can program the future metabolic health of the newborn? More precisely, we want to unravel the mechanism by which the embryo's metabolites, in response to the culture environment, direct the epigenetic programming process on histones and DNA (methylation) including the mitochondria DNA. Understanding such a fundamental question can lead to targeted improvement in culture conditions leading to better health in in vitro produced bovine embryos. To initiate this collaboration, the Brazilian laboratory will prepare embryo's samples in 3 different metabolic conditions to assess phenotypic and biochemical consequences and then one student from Milazzotto's lab will come to Canada to perform the epigenetic analysis for which Dr Sirard's lab has developed new approaches for micro samples as well as for mitochondrial DNA. These results will demonstrate the possibility and the value of using metabolic changes to modify the epigenome of early embryos in a very important domestic animal species.